Anti-PKC gamma (phospho T655) 抗体 (ab5796)

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Western blot - Anti-PKC gamma (phospho T655) antibody (ab5796)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC gamma (phospho T655) antibody (ab5796)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC gamma (phospho T655) antibody (ab5796)

Key features and details

  • Rabbit polyclonal to PKC gamma (phospho T655)
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-PKC gamma (phospho T655) antibody
    PKC gamma 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to PKC gamma (phospho T655)

  • 由来種

    Rabbit

  • 特異性

    PKC alpha (69%) and beta 2 (62%) may cross-react in cells expressing high levels of these proteins.

  • アプリケーション

    適用あり: IHC-P, WBmore details

  • 種交差性

    交差種: Mouse, Human
    交差が予測される動物種: Rat

  • 免疫原

    Synthetic peptide corresponding to PKC gamma (phospho T655).

  • ポジティブ･コントロール

    • WB: HeLa cells treated with PMA, a phorbol ester. IHC-P: Mouse cerebellum tissue, human cerebellum tissue.

  • 特記事項


    PKC gamma is an 80 kDa member of the conventional group (cPKCs: sensitive to calcium, diacylglycerol and phorbol esters) of the PKC family of serine/threonine family kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival and transcriptional regulation. PKC gamma plays a key role in neuronal signal transduction and is translocated from the nucleus to the cytoplasm upon activation by phorbol ester, where in epithelial cells it has been implicated in regulating intracellular communication. The activation loop threonine (threonine 514 in PKC gamma) of conventional PKCs is phosphorylated by phosphoinositide-dependent kinase-1 (PDK1), which is necessary for their autophosphorylation, a critical step in the generation of a catalytically mature enzyme. Threonine 655 is an autophosphorylation site in the carboxy-terminus of PKC gamma.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

  • バッファー

    pH: 7.30
    Preservative: 0.05% Sodium azide
    Constituents: PBS, 0.1% BSA
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • 特記事項（精製）

    The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated PKC gamma. The final product is generated by affinity chromatography using a PKC gamma-derived peptide that is phosphorylated at threonine 655.

  • 一次抗体 備考

    PKC gamma is an 80 kDa member of the conventional group (cPKCs: sensitive to calcium, diacylglycerol and phorbol esters) of the PKC family of serine/threonine family kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival and transcriptional regulation. PKC gamma plays a key role in neuronal signal transduction and is translocated from the nucleus to the cytoplasm upon activation by phorbol ester, where in epithelial cells it has been implicated in regulating intracellular communication. The activation loop threonine (threonine 514 in PKC gamma) of conventional PKCs is phosphorylated by phosphoinositide-dependent kinase-1 (PDK1), which is necessary for their autophosphorylation, a critical step in the generation of a catalytically mature enzyme. Threonine 655 is an autophosphorylation site in the carboxy-terminus of PKC gamma.

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab5796の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
1/10 - 1/50.
WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 80 kDa.
特記事項
IHC-P
1/10 - 1/50.
WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 80 kDa.

ターゲット情報

  • 機能

    This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme.
    PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.

  • 組織特異性

    Expressed in Purkinje cells of the cerebellar cortex.

  • 関連疾患

    Defects in PRKCG are the cause of spinocerebellar ataxia type 14 (SCA14) [MIM:605361]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA14 is an autosomal dominant cerebellar ataxia (ADCA).

  • 配列類似性

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 C2 domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • KPCG_HUMAN antibody
    • MGC57564 antibody
    • OTTHUMP00000067291 antibody
    • PKC-gamma antibody
    • PKCC antibody
    • PKCG antibody
    • PRKCG antibody
    • Protein kinase C gamma antibody
    • Protein kinase C gamma polypeptide antibody
    • Protein kinase C gamma type antibody
    • Protein kinase C, gamma antibody
    • SCA 14 antibody
    • SCA14 antibody
    see all

画像

  • Western blot - Anti-PKC gamma (phospho T655) antibody (ab5796)
    Western blot - Anti-PKC gamma (phospho T655) antibody (ab5796)

    Peptide Competition and Phosphatase treatment: Lysates prepared from HeLa cells stimulated with PMA were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either left untreated (1-11) or treated with lambda (ë) phosphatase (12), blocked with a 3% low-fat milk-TBST buffer overnight at 4°C, and incubated with 0.50 µg/mL ab5796 antibody for two hours at room temperature in a 3% BSA TBST buffer, following prior incubation with: no peptide (1, 11, 12), the non-phosphopeptide corresponding to the immunogen (2), a generic phosphothreonine containing peptide (3), the phosphopeptide immunogen (4), or, the phosphopeptide corresponding to the immunogen from other PKC isoforms (5-10). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase  and signals were detected using the Tropix WesternStarTM method. The data show that the peptide corresponding to PKC gamma [pT655] blocks the an

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC gamma (phospho T655) antibody (ab5796)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC gamma (phospho T655) antibody (ab5796)

    Paraffin embedded human cerebellum tissue (right) stained for PKC gamma using ab5796 at 1/20 dilution in immunohistochemical analysis. Negative control without primary antibody (left).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC gamma (phospho T655) antibody (ab5796)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC gamma (phospho T655) antibody (ab5796)

    Paraffin embedded mouse cerebellum tissue (right) stained for PKC gamma using ab5796 at 1/100 dilution in immunohistochemical analysis. Negative control without primary antibody (left).

参考文献 (1)

ab5796 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab5796 は 1 報の論文で使用されています。

  • Fujita K  et al. Targeting Tyro3 ameliorates a model of PGRN-mutant FTLD-TDP via tau-mediated synaptic pathology. Nat Commun 9:433 (2018). PubMed: 29382817

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