Anti-PKC gamma (phospho T655) 抗体 (ab5796)
Key features and details
- Rabbit polyclonal to PKC gamma (phospho T655)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-PKC gamma (phospho T655) antibody
PKC gamma 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PKC gamma (phospho T655) -
由来種
Rabbit -
特異性
PKC alpha (69%) and beta 2 (62%) may cross-react in cells expressing high levels of these proteins. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic phosphopeptide derived from a region of human PKC gamma that contains threonine 655.
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ポジティブ･コントロール
- Hela cells treated with PMA, a phorbol ester.
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特記事項
PKC gamma is an 80 kDa member of the conventional group (cPKCs: sensitive to calcium, diacylglycerol and phorbol esters) of the PKC family of serine/threonine family kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival and transcriptional regulation. PKC gamma plays a key role in neuronal signal transduction and is translocated from the nucleus to the cytoplasm upon activation by phorbol ester, where in epithelial cells it has been implicated in regulating intracellular communication. The activation loop threonine (threonine 514 in PKC gamma) of conventional PKCs is phosphorylated by phosphoinositide-dependent kinase-1 (PDK1), which is necessary for their autophosphorylation, a critical step in the generation of a catalytically mature enzyme. Threonine 655 is an autophosphorylation site in the carboxy-terminus of PKC gamma.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated PKC gamma. The final product is generated by affinity chromatography using a PKC gamma-derived peptide that is phosphorylated at threonine 655. -
一次抗体 備考
PKC gamma is an 80 kDa member of the conventional group (cPKCs: sensitive to calcium, diacylglycerol and phorbol esters) of the PKC family of serine/threonine family kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival and transcriptional regulation. PKC gamma plays a key role in neuronal signal transduction and is translocated from the nucleus to the cytoplasm upon activation by phorbol ester, where in epithelial cells it has been implicated in regulating intracellular communication. The activation loop threonine (threonine 514 in PKC gamma) of conventional PKCs is phosphorylated by phosphoinositide-dependent kinase-1 (PDK1), which is necessary for their autophosphorylation, a critical step in the generation of a catalytically mature enzyme. Threonine 655 is an autophosphorylation site in the carboxy-terminus of PKC gamma. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab5796 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 80 kDa. |
ターゲット情報
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機能
This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme.
PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. -
組織特異性
Expressed in Purkinje cells of the cerebellar cortex. -
関連疾患
Defects in PRKCG are the cause of spinocerebellar ataxia type 14 (SCA14) [MIM:605361]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA14 is an autosomal dominant cerebellar ataxia (ADCA). -
配列類似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 C2 domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain. - Information by UniProt
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参照データベース
- Entrez Gene: 5582 Human
- Entrez Gene: 18752 Mouse
- Entrez Gene: 24681 Rat
- Omim: 176980 Human
- SwissProt: P05129 Human
- SwissProt: P63318 Mouse
- SwissProt: P63319 Rat
- Unigene: 631564 Human
see all -
別名
- KPCG_HUMAN antibody
- MGC57564 antibody
- OTTHUMP00000067291 antibody
see all
画像
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Western blot - Anti-PKC gamma (phospho T655) antibody (ab5796)
Peptide Competition and Phosphatase treatment: Lysates prepared from HeLa cells stimulated with PMA were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either left untreated (1-11) or treated with lambda (ë) phosphatase (12), blocked with a 3% low-fat milk-TBST buffer overnight at 4°C, and incubated with 0.50
µ g/mL ab5796 antibody for two hours at room temperature in a 3% BSA TBST buffer, following prior incubation with: no peptide (1, 11, 12), the non-phosphopeptide corresponding to the immunogen (2), a generic phosphothreonine containing peptide (3), the phosphopeptide immunogen (4), or, the phosphopeptide corresponding to the immunogen from other PKC isoforms (5-10). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and signals were detected using the Tropix WesternStarTM method. The data show that the peptide corresponding to PKC gamma [pT655] blocks the antibody signal while the peptides corresponding to PKC isoforms beta 1 [pT642], delta [pS645], epsilon [pT710] and eta [pT655] do not completely block the antibody signal, thereby demonstrating the specificity of the antibody. However, peptides corresponding to PKC isoforms alpha [pT638] and beta 2 [pT641] do block the antibody signal, indicating that the ab5796 antibody may cross-react with their respective sites. The data also show that phosphatase stripping eliminates the signal, verifying that the antibody is phosphospecific.
Peptide Competition and Phosphatase treatment: Lysates prepared from HeLa cells stimulated with PMA were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either left untreated (1-11) or treated with lambda (ë) phosphatase (12), blocked with a 3% low-fat milk-TBST buffer overnight at 4°C, and incubated with 0.50 µg/mL ab5796 antibody for two hours at room temperature in a 3% BSA TBST buffer, following prior incubation with: no peptide (1, 11, 12), the non-phosphopeptide corresponding to the immunogen (2), a generic phosphothreonine containing peptide (3), the phosphopeptide immunogen (4), or, the phosphopeptide corresponding to the immunogen from other PKC isoforms (5-10). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and signals were detected using the Tropix WesternStarTM method. The data show that the peptide corresponding to PKC gamma [pT655] blocks the antibody signal while the peptides corresponding to PKC isoforms beta 1 [pT642], delta [pS645], epsilon [pT710] and eta [pT655] do not completely block the antibody signal, thereby demonstrating the specificity of the antibody. However, peptides corresponding to PKC isoforms alpha [pT638] and beta 2 [pT641] do block the antibody signal, indicating that the ab5796 antibody may cross-react with their respective sites. The data also show that phosphatase stripping eliminates the signal, verifying that the antibody is phosphospecific.
データシートおよび資料
参考文献 (1)
ab5796 は 1 報の論文で使用されています。
- Fujita K et al. Targeting Tyro3 ameliorates a model of PGRN-mutant FTLD-TDP via tau-mediated synaptic pathology. Nat Commun 9:433 (2018). PubMed: 29382817
