Key features and details
- Rabbit polyclonal to PKC gamma
- Suitable for: ICC/IF, ELISA, IHC-P, IHC-Fr, WB
- Reacts with: Human
- Isotype: IgG
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
バッファーPreservative: 0.05% Sodium azide
Concentration information loading...
Our Abpromise guarantee covers the use of ab4144 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Immunohistochemistry: 1/250 (using horseradish peroxidase or alkaline phosphatase).
Western blot (using horseradish peroxidase or alkaline phosphatase): 1/250. Predicted molecular weight: 84 kDa.
Not tested in other applications.
Optimal dilutions / concentrations should be determined by the end user.
With a more sensitive detection system, such as enhanced chemiluminescence or ELISA, the antibody may be diluted further.
It has also been reported to be useful for immunoprecipitation of PKC gamma.
機能This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme.
PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
組織特異性Expressed in Purkinje cells of the cerebellar cortex.
関連疾患Defects in PRKCG are the cause of spinocerebellar ataxia type 14 (SCA14) [MIM:605361]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA14 is an autosomal dominant cerebellar ataxia (ADCA).
配列類似性Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 C2 domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain.
- Information by UniProt
- KPCG_HUMAN antibody
- MGC57564 antibody
- OTTHUMP00000067291 antibody