Anti-PKC gamma 抗体 (ab4144)

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Key features and details

  • Rabbit polyclonal to PKC gamma
  • Suitable for: ELISA, IHC-Fr, IHC-P, ICC/IF, WB
  • Reacts with: Human
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-PKC alpha + beta 2 + gamma antibody [EPR18104] (ab184746)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-PKC gamma antibody
    PKC gamma 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to PKC gamma

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: ELISA, IHC-Fr, IHC-P, ICC/IF, WBmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mammals

  • 免疫原

    Synthetic peptide:

    FEACNYPLELYEVSRYGPSSS

    , corresponding to amino acids 302-322 of Human PKC gamma.
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab4144の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ELISA
IHC-Fr
IHC-P
ICC/IF (1)
WB (1)
特記事項
追加情報
ELISA: assay dependent.
Immunohistochemistry: 1/250 (using horseradish peroxidase or alkaline phosphatase).
Western blot (using horseradish peroxidase or alkaline phosphatase): 1/250. Predicted molecular weight: 84 kDa.
Not tested in other applications.

Optimal dilutions / concentrations should be determined by the end user.

With a more sensitive detection system, such as enhanced chemiluminescence or ELISA, the antibody may be diluted further.

It has also been reported to be useful for immunoprecipitation of PKC gamma.

ターゲット情報

  • 機能

    This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme.
    PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.

  • 組織特異性

    Expressed in Purkinje cells of the cerebellar cortex.

  • 関連疾患

    Defects in PRKCG are the cause of spinocerebellar ataxia type 14 (SCA14) [MIM:605361]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA14 is an autosomal dominant cerebellar ataxia (ADCA).

  • 配列類似性

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 C2 domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
  • Information by UniProt

  • 参照データベース

    • 別名

      • KPCG_HUMAN antibody
      • MGC57564 antibody
      • OTTHUMP00000067291 antibody
      • PKC-gamma antibody
      • PKCC antibody
      • PKCG antibody
      • PRKCG antibody
      • Protein kinase C gamma antibody
      • Protein kinase C gamma polypeptide antibody
      • Protein kinase C gamma type antibody
      • Protein kinase C, gamma antibody
      • SCA 14 antibody
      • SCA14 antibody
      see all

    参考文献 (0)

    ab4144 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab4144 は論文での使用が確認できていません。

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    1-2 of 2 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-PKC gamma antibody

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HT1080 Cell line)
    Specification
    HT1080 Cell line
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.1% Triton X-100
    Read More

    Abcam user community

    Verified customer

    投稿 Feb 17 2010

    Western blot abreview for Anti-PKC gamma antibody

     Average
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HT1080 Cell line)
    Loading amount
    20000 cells
    Specification
    HT1080 Cell line
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    投稿 Feb 10 2010

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