Anti-PKC gamma 抗体 (ab4144)
Key features and details
- Rabbit polyclonal to PKC gamma
- Suitable for: ICC/IF, ELISA, IHC-P, IHC-Fr, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-PKC gamma antibody
PKC gamma 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PKC gamma -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, ELISA, IHC-P, IHC-Fr, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mammals
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免疫原
Synthetic peptide:
FEACNYPLELYEVSRYGPSSS
, corresponding to amino acids 302-322 of Human PKC gamma.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide -
Concentration information loading... -
精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab4144 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | ||
| ELISA | ||
| IHC-P | ||
| IHC-Fr | ||
| WB |
追加情報
ELISA: assay dependent.
Immunohistochemistry: 1/250 (using horseradish peroxidase or alkaline phosphatase).
Western blot (using horseradish peroxidase or alkaline phosphatase): 1/250. Predicted molecular weight: 84 kDa.
Not tested in other applications.
Optimal dilutions / concentrations should be determined by the end user.
With a more sensitive detection system, such as enhanced chemiluminescence or ELISA, the antibody may be diluted further.
It has also been reported to be useful for immunoprecipitation of PKC gamma.
Immunohistochemistry: 1/250 (using horseradish peroxidase or alkaline phosphatase).
Western blot (using horseradish peroxidase or alkaline phosphatase): 1/250. Predicted molecular weight: 84 kDa.
Not tested in other applications.
Optimal dilutions / concentrations should be determined by the end user.
With a more sensitive detection system, such as enhanced chemiluminescence or ELISA, the antibody may be diluted further.
It has also been reported to be useful for immunoprecipitation of PKC gamma.
ターゲット情報
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機能
This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme.
PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. -
組織特異性
Expressed in Purkinje cells of the cerebellar cortex. -
関連疾患
Defects in PRKCG are the cause of spinocerebellar ataxia type 14 (SCA14) [MIM:605361]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA14 is an autosomal dominant cerebellar ataxia (ADCA). -
配列類似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 C2 domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain. - Information by UniProt
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参照データベース
- Entrez Gene: 5582 Human
- Omim: 176980 Human
- SwissProt: P05129 Human
- Unigene: 631564 Human
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別名
- KPCG_HUMAN antibody
- MGC57564 antibody
- OTTHUMP00000067291 antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-PKC gamma antibody (ab4144)
プロトコール
データシートおよび資料
参考文献 (0)
ab4144 は論文での使用が確認できていません。
