Anti-PKC delta (phospho S299) 抗体 [EPNCI119] (ab133456)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPNCI119] to PKC delta (phospho S299)
- Suitable for: WB, IHC-P
- Reacts with: Human
製品の概要
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製品名
Anti-PKC delta (phospho S299) antibody [EPNCI119]
PKC delta 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPNCI119] to PKC delta (phospho S299) -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt or ICC/IF -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: MCF7 treated with 100nM Calyculin A; IHC-P: Human bladder cancer tissue.
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特記事項
This product has switched from a hybridoma to recombinant production method on 29th January 2024.
This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institutes Center for Cancer Research and the lab of Peter Blumberg. View antibodies from NCI Center for Cancer Research Collaboration.
The auto-phosphorylation of PKC delta at pS299 targeted by ab133456 was identified in the following paper: FEBS Lett. 2007 Jul 24;581(18):3377-81. Novel phosphorylation site markers of protein kinase C delta activation. Durgan J1, Michael N, Totty N, Parker PJ.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPNCI119 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab133456の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (3) |
1/5000. Detects a band of approximately 78 kDa (predicted molecular weight: 78 kDa).
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IHC-P |
1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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WB
1/5000. Detects a band of approximately 78 kDa (predicted molecular weight: 78 kDa). |
IHC-P
1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
This is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. May play a role in antigen-dependent control of B-cell function. Phosphorylates MUC1 in the C-terminal and regulates the interaction between MUC1 and beta-catenin. -
配列類似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 C2 domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain. -
ドメイン
The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor.
The C2 domain is a non-calcium binding domain. It binds proteins containing phosphotyrosine in a sequence-specific manner. -
翻訳後修飾
Phosphorylated on Thr-507, within the activation loop. Autophosphorylated and/or phosphorylated. Although the Thr-507 phosphorylation occurs it is not a prerequisite for enzymatic activity. -
細胞内局在
Cytoplasm. Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 5580 Human
- Omim: 176977 Human
- SwissProt: Q05655 Human
- Unigene: 155342 Human
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別名
- CVID9 antibody
- D14Ertd420e antibody
- Kinase PKC delta antibody
see all
画像
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All lanes : Anti-PKC delta (phospho S299) antibody [EPNCI119] (ab133456) at 1/5000 dilution
Lane 1 : Untreated MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : MCF7 treated with 100nM Calyculin A for 30min whole cell lysate
Lane 3 : MCF7 treated with 100nM Calyculin A for 30min whole cell lysate, then the membrane was incubated with phosphatase
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 78 kDa
Observed band size: 78 kDa
Exposure time: 20 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
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Immunohistochemical analysis of paraffin-embedded Human bladder cancer tissue labeling PKC delta with ab133456 at 1/5000 (0.077 µg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human bladder cancer without alkaline phosphatase treatment (image A). No signal was detected when tissues were treated with alkaline phosphatase (image B). The human bladder cancer sections were performed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The sections were using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins, then labeling ab133456 at 1/5000 dilution for 30 mins at room temperature. Counterstained with hematoxylin. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab133456 は 5 報の論文で使用されています。
- Zhou B et al. B7-H4 expression is upregulated by PKCδ activation and contributes to PKCδ-induced cell motility in colorectal cancer. Cancer Cell Int 22:147 (2022). PubMed: 35410218
- Wang N et al. Selective ERK1/2 agonists isolated from Melia azedarach with potent anti-leukemic activity. BMC Cancer 19:764 (2019). PubMed: 31375085
- Li H & Chen C Quercetin Has Antimetastatic Effects on Gastric Cancer Cells via the Interruption of uPA/uPAR Function by Modulating NF-?b, PKC-d, ERK1/2, and AMPKa. Integr Cancer Ther 17:511-523 (2018). PubMed: 28627240
- Kelsey JS et al. Biological activity of the bryostatin analog Merle 23 on mouse epidermal cells and mouse skin. Mol Carcinog 55:2183-2195 (2016). PubMed: 26859836
- Song X et al. RasGRPs are targets of the anti-cancer agent ingenol-3-angelate. PLoS One 8:e72331 (2013). WB ; Human . PubMed: 23991094