Application
Western blot
Sample
Human Cell lysate - whole cell (human pancreatic cancer cell lines)
Loading amount
30 µg
Specification
human pancreatic cancer cell lines
Gel Running Conditions
Non-reduced Denaturing (7.5%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Dilution
1/1000
Incubation time
8 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST
Secondary antibody
Name
Non-Abcam antibody was used: Amersham-HRP Sheep antiMouse
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 76 kDa
Additional data
Additional Notes
Following cell lines were used:
Line1: Panc1
Line2: MCF-7
Line3: MiaPaCa
Line1: Panc1
Line2: MCF-7
Line3: MiaPaCa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Pawel Mazur
Verified customer
投稿 Jul 31 2010