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  1. Link

    pkc-antibody-mc5-ab31.pdf

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Signal Transduction Protein Phosphorylation Ser / Thr Kinases PKC
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Anti-PKC 抗体 [MC5] (ab31)

  • Datasheet
  • SDS
Reviews (6)Q&A (4)References (37)

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Abpromise

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Western blot - Anti-PKC antibody [MC5] (ab31)
  • Flow Cytometry - Anti-PKC antibody [MC5] (ab31)

Key features and details

  • Mouse monoclonal [MC5] to PKC
  • Suitable for: WB, Flow Cyt
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG2a

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-PKC alpha + beta 2 + gamma antibody [EPR18104] (ab184746)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-PKC antibody [MC5]
    PKC 一次抗体 製品一覧
  • 製品の詳細

    Mouse monoclonal [MC5] to PKC
  • 由来種

    Mouse
  • 特異性

    This antibody reacts with PKC alpha, crossreactivity to PKC beta isoforms can be observed.

  • アプリケーション

    適用あり: WB, Flow Cytmore details
  • 種交差性

    交差種: Mouse, Rat, Human
  • 免疫原

    Full length protein corresponding to Cow PKC. Purified bovine brain protein kinase C

  • ポジティブ・コントロール

    • WB: Calpain treated PKC. Rat adipocyte homogenate. 3T3-L1 homogenate. Purified microsomes from 3T3-L1 cells. Flow Cytometry: Jurkat cells. ICC/IF: Vero and HEK-293T cells. IHC-Fr: mouse spleen and duodenum tissue. IHC-P: Human skin papilloma tissue.
  • 特記事項

    It should be possible to selectively block degradation of PKC without affecting the membrane associated activation. This will allow an assessment of the role of proteolysis in the activation of the protein kinase pathway.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Concentration information loading...
  • 精製度

    Protein A purified
  • ポリ/モノ

    モノクローナル
  • クローン名

    MC5
  • ミエローマ

    P3x63
  • アイソタイプ

    IgG2a
  • 軽鎖の種類

    unknown
  • 研究分野

    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • PKC
    • Cancer
    • Signal transduction
    • Protein phosphorylation
    • Serine/threonine kinases
    • Other
    • Cardiovascular
    • Heart
    • Hypertrophy
    • Other
    • Neuroscience
    • Development
    • Neuroscience
    • Processes

関連製品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab31の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (2)
Use at an assay dependent concentration.
Flow Cyt
Use 1µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

特記事項
WB
Use at an assay dependent concentration.
Flow Cyt
Use 1µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

ターゲット情報

  • 機能

    Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that is involved in positive and negative regulation of cell proliferation, apoptosis, differentiation, migration and adhesion, tumorigenesis, cardiac hypertrophy, angiogenesis, platelet function and inflammation, by directly phosphorylating targets such as RAF1, BCL2, CSPG4, TNNT2/CTNT, or activating signaling cascade involving MAPK1/3 (ERK1/2) and RAP1GAP. Involved in cell proliferation and cell growth arrest by positive and negative regulation of the cell cycle. Can promote cell growth by phosphorylating and activating RAF1, which mediates the activation of the MAPK/ERK signaling cascade, and/or by up-regulating CDKN1A, which facilitates active cyclin-dependent kinase (CDK) complex formation in glioma cells. In intestinal cells stimulated by the phorbol ester PMA, can trigger a cell cycle arrest program which is associated with the accumulation of the hyper-phosphorylated growth-suppressive form of RB1 and induction of the CDK inhibitors CDKN1A and CDKN1B. Exhibits anti-apoptotic function in glioma cells and protects them from apoptosis by suppressing the p53/TP53-mediated activation of IGFBP3, and in leukemia cells mediates anti-apoptotic action by phosphorylating BCL2. During macrophage differentiation induced by macrophage colony-stimulating factor (CSF1), is translocated to the nucleus and is associated with macrophage development. After wounding, translocates from focal contacts to lamellipodia and participates in the modulation of desmosomal adhesion. Plays a role in cell motility by phosphorylating CSPG4, which induces association of CSPG4 with extensive lamellipodia at the cell periphery and polarization of the cell accompanied by increases in cell motility. Is highly expressed in a number of cancer cells where it can act as a tumor promoter and is implicated in malignant phenotypes of several tumors such as gliomas and breast cancers. Negatively regulates myocardial contractility and positively regulates angiogenesis, platelet aggregation and thrombus formation in arteries. Mediates hypertrophic growth of neonatal cardiomyocytes, in part through a MAPK1/3 (ERK1/2)-dependent signaling pathway, and upon PMA treatment, is required to induce cardiomyocyte hypertrophy up to heart failure and death, by increasing protein synthesis, protein-DNA ratio and cell surface area. Regulates cardiomyocyte function by phosphorylating cardiac troponin T (TNNT2/CTNT), which induces significant reduction in actomyosin ATPase activity, myofilament calcium sensitivity and myocardial contractility. In angiogenesis, is required for full endothelial cell migration, adhesion to vitronectin (VTN), and vascular endothelial growth factor A (VEGFA)-dependent regulation of kinase activation and vascular tube formation. Involved in the stabilization of VEGFA mRNA at post-transcriptional level and mediates VEGFA-induced cell proliferation. In the regulation of calcium-induced platelet aggregation, mediates signals from the CD36/GP4 receptor for granule release, and activates the integrin heterodimer ITGA2B-ITGB3 through the RAP1GAP pathway for adhesion. During response to lipopolysaccharides (LPS), may regulate selective LPS-induced macrophage functions involved in host defense and inflammation. But in some inflammatory responses, may negatively regulate NF-kappa-B-induced genes, through IL1A-dependent induction of NF-kappa-B inhibitor alpha (NFKBIA/IKBA). Upon stimulation with 12-O-tetradecanoylphorbol-13-acetate (TPA), phosphorylates EIF4G1, which modulates EIF4G1 binding to MKNK1 and may be involved in the regulation of EIF4E phosphorylation. Phosphorylates KIT, leading to inhibition of KIT activity. Phosphorylates ATF2 which promotes cooperation between ATF2 and JUN, activating transcription.
  • 配列類似性

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 C2 domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
  • 細胞内局在

    Cytoplasm. Cell membrane. Mitochondrion membrane. Nucleus.
  • Target information above from: UniProt accession P17252 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 5578 Human
    • Entrez Gene: 5579 Human
    • Entrez Gene: 5580 Human
    • Entrez Gene: 5581 Human
    • Entrez Gene: 5582 Human
    • Entrez Gene: 5590 Human
    • Entrez Gene: 18750 Mouse
    • Entrez Gene: 18751 Mouse
    • Entrez Gene: 18752 Mouse
    • Entrez Gene: 18753 Mouse
    • Entrez Gene: 18754 Mouse
    • Entrez Gene: 18762 Mouse
    • Entrez Gene: 170538 Rat
    • Entrez Gene: 24680 Rat
    • Entrez Gene: 24681 Rat
    • Entrez Gene: 25023 Rat
    • Entrez Gene: 25522 Rat
    • Entrez Gene: 29340 Rat
    • Omim: 176970 Human
    • Omim: 176982 Human
    • SwissProt: P05129 Human
    • SwissProt: P05771 Human
    • SwissProt: P17252 Human
    • SwissProt: Q02156 Human
    • SwissProt: Q05513 Human
    • SwissProt: Q05655 Human
    • SwissProt: P20444 Mouse
    • SwissProt: P23298 Mouse
    • SwissProt: P63318 Mouse
    • SwissProt: P68404 Mouse
    • SwissProt: Q02956 Mouse
    • SwissProt: P05696 Rat
    • SwissProt: P09217 Rat
    • SwissProt: P63319 Rat
    • SwissProt: P68403 Rat
    • Unigene: 531704 Human
    • Unigene: 708867 Human
    • Unigene: 222178 Mouse
    • Unigene: 86669 Rat
    see all
  • 別名

    • KPCA_HUMAN antibody
    • PKC alpha antibody
    • PKC beta antibody
    • PKC delta antibody
    • PKC epsilon antibody
    • PKC gamma antibody
    • PKC zeta antibody
    • PKC-A antibody
    • PKC-alpha antibody
    • PKC2 antibody
    • PKCA antibody
    • PKCB antibody
    • PKCD antibody
    • PKCE antibody
    • PKCG antibody
    • PRKCA antibody
    • PRKCB antibody
    • PRKCB1 antibody
    • PRKCB2 antibody
    • PRKCD antibody
    • PRKCE antibody
    • PRKCG antibody
    • PRKCZ antibody
    • Protein kinase C alpha antibody
    • Protein kinase C alpha type antibody
    • Protein kinase C antibody
    • Protein kinase C beta antibody
    • Protein kinase C delta antibody
    • Protein kinase C epsilon antibody
    • Protein kinase C gamma antibody
    • Protein kinase C zeta antibody
    see all

画像

  • Western blot - Anti-PKC antibody [MC5] (ab31)
    Western blot - Anti-PKC antibody [MC5] (ab31)
    All lanes : Anti-PKC antibody [MC5] (ab31)

    Lane 1 : Calpain treated PKC
    Lane 2 : Homogenate from rat adipocytes
    Lane 3 : Homogenate from 3T3-L1 cells
    Lane 4 : Purified microsomes from 3T3-L1 cells


    Review by Mikael Rutberg submitted 2 September 2004.

  • Flow Cytometry - Anti-PKC antibody [MC5] (ab31)
    Flow Cytometry - Anti-PKC antibody [MC5] (ab31)

    Overlay histogram showing Jurkat (Human T cell leukemia cell line from peripheral blood) cells stained with ab31 (red line). The cells were fixed with 80% methanol (5 minutes) and then permeabilized with 0.1% PBS-Tween for 20 minutes. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab31, 1 μg/1x106 cells) for 30 minutes at 22°C. The secondary antibody used was a goat anti-mouse Alexa Fluor® 488 (IgG; H+L) ab150113 at 1/2000 dilution for 30 minutes at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1 μg/1x106 cells) used under the same conditions. Unlabeled sample (blue line) was used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

    This antibody gave a positive signal in HL-60 (Human promyelocytic leukemia cell line) cells fixed with 4% paraformaldehyde (10 minutes)/permeabilized with 0.1% PBS-Tween for 20 minutes used under the same conditions.

プロトコール

  • Flow cytometry protocols
  • Western blot protocols

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (37)

ab31 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab31 は 37 報の論文で使用されています。

  • Hao EY  et al. The relationship between the mTOR signaling pathway and ovarian aging in peak-phase and late-phase laying hens. Poult Sci 100:334-347 (2021). PubMed: 33357698
  • Burger CA  et al. LKB1 and AMPK instruct cone nuclear position to modify visual function. Cell Rep 34:108698 (2021). PubMed: 33535040
  • Pourhoseini S  et al. Neurofascin Is a Novel Component of Rod Photoreceptor Synapses in the Outer Retina. Front Neural Circuits 15:635849 (2021). PubMed: 33643000
  • Yi MH  et al. Optogenetic activation of spinal microglia triggers chronic pain in mice. PLoS Biol 19:e3001154 (2021). PubMed: 33739978
  • Xu J  et al. Oxidative stress induced by NOX2 contributes to neuropathic pain via plasma membrane translocation of PKCe in rat dorsal root ganglion neurons. J Neuroinflammation 18:106 (2021). PubMed: 33952299
View all Publications for this product

レビューと Q&A

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レビューを送る 質問を送る

1-10 of 10 Abreviews or Q&A

Western blot abreview for Anti-PKC antibody [MC5]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (human pancreatic cancer cell lines)
Loading amount
30 µg
Specification
human pancreatic cancer cell lines
Gel Running Conditions
Non-reduced Denaturing (7.5%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Pawel Mazur

Verified customer

投稿 Jul 31 2010

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PKC antibody [MC5]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Skin papiloma)
Specification
Skin papiloma
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jul 15 2010

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PKC antibody [MC5]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (mouse pancreatic neoplasia)
Specification
mouse pancreatic neoplasia
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
No
Blocking step
MOM kit blocking agaent as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jul 14 2010

Immunohistochemistry (Frozen sections) abreview for Anti-PKC antibody [MC5]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (pancreatic tisse and tumor)
Specification
pancreatic tisse and tumor
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jul 09 2010

Immunohistochemistry (Frozen sections) abreview for Anti-PKC antibody [MC5]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Mouse spleen and duodenum)
Specification
Mouse spleen and duodenum
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jul 09 2010

Western blot abreview for Anti-PKC antibody [MC5]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Pancreatic cancer)
Loading amount
35 µg
Specification
Pancreatic cancer
Gel Running Conditions
Non-reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Pawel Mazur

Verified customer

投稿 Jun 04 2010

Question

Do you have any information whether this antibody does recongize other isoforms of PKC? Can you please confirm which isoforms of PKC have been tested as well?

Read More

Abcam community

Verified customer

Asked on Nov 18 2010

Answer

Indeed, this antibody is a clone and is used by many researchers. However I could not conclude on its specificity. Indeed, some sources quote that this antibody is specific for PKC alpha, whereas other state that there might be crossreactivity with PKC beta isoforms. In summary: we can guarantee that this antibody will recognize PKC alpha, we can however not exclude crossreactivity with PKC beta isoformes. Please do not hesitate to contact us should you have any questions or concerns.

Read More

Abcam Scientific Support

Answered on Dec 10 2010

Question

What type of tissue is recommended as a positive control for IHC-P?

Read More

Abcam community

Verified customer

Asked on Jan 18 2006

Answer

Thank you for your enquiry. For ab4127, this antibody was validated on tPA induced myeloid cells for IHC. Also, melanocytes have been known to express PKC alpha and alternatively brain tissue is typically inherently expressive. For ab31, I regret to inform you that this antibody has not yet been tested for application in IHC. I have updated the online datasheet to reflect this, and apologize for this. Please contact us again if you have any additional questions or concerns.

Read More

Abcam Scientific Support

Answered on Jan 20 2006

Question

Could you tell us which PKC isoform(s) would your PKC antibody [MC5] (ab31) recognize? We are interested in using immunofluorescence to determine the spatial distribution of PKC alpha. Do you suggest any other Ab?

Read More

Abcam community

Verified customer

Asked on Apr 28 2005

Answer

I apologize for the delay in responding to your enquiry. The originator of this antibody attests that this antibody has been found to recognize Protein kinase Ca. Epitope: within the variable region V3 PKCa (the site of limited proteolysis on the native enzyme). If you require further information, please contact us again and we will gladly assist you.

Read More

Abcam Scientific Support

Answered on May 11 2005

Question

any suggestions for a range of dilutions for Western blot?

Read More

Abcam community

Verified customer

Asked on Jul 09 2002

Answer

We have no information about the dilutions originally used with this antibody. I would suggest trying the antibody at 1:1000, 1:250 and 1:2000 initially, the ideal dilution will depend on the details of your experiments.

Read More

Abcam Scientific Support

Answered on Jul 11 2002

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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