Anti-PKC alpha 抗体 [133] - BSA and Azide free (ab284403)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [133] to PKC alpha - BSA and Azide free
- Suitable for: IHC-P, WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-PKC alpha antibody [133] - BSA and Azide free
PKC alpha 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [133] to PKC alpha - BSA and Azide free -
由来種
Mouse -
特異性
ab11723 recognises the alpha isoform of PKC.
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アプリケーション
適用あり: IHC-P, WBmore details
適用なし: ICC/IF -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
(Peptide available asab55672) -
ポジティブ・コントロール
- WB: HAP1, K562, C6, HeLa, NIH/3T3 and HEK-293 cell lysate. Human kidney tissue lysate. Mouse and rat brain and spleen tissue lysate. Recombinant human PKC alpha protein (Active) (ab55672). IHC-P: Human, mouse and rat retina tissue.
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特記事項
This product has switched from a hybridoma to recombinant production method on 25th July 2021.
ab284403 is the carrier-free version of ab11723.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.20
Constituent: 100% PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
133 -
ミエローマ
Sp2/0 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab284403の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 76 kDa.Can be blocked with Recombinant human PKC alpha protein (Active) (ab55672).
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特記事項 |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 76 kDa.Can be blocked with Recombinant human PKC alpha protein (Active) (ab55672). |
ターゲット情報
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機能
This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme. May play a role in cell motility by phosphorylating CSPG4.
PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. -
配列類似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 C2 domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain. -
細胞内局在
Cytoplasm. Cell membrane. Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 5578 Human
- Entrez Gene: 18750 Mouse
- Entrez Gene: 24680 Rat
- Omim: 176960 Human
- SwissProt: P17252 Human
- SwissProt: P20444 Mouse
- SwissProt: P05696 Rat
- Unigene: 531704 Human
see all -
別名
- AAG6 antibody
- Aging associated gene 6 antibody
- aPKC antibody
see all
画像
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This data was developed using ab11723 the same antibody clone in a different buffer formulation.
This image was generated from the hybridoma version of the product.
Lanes 1, 5 and 9: Wild-type HAP1 cell lysate (20 µg)
Lanes 2, 6 and 10: PKC alpha knockout HAP1 cell lysate (20 µg)
Lanes 3, 7 and 11: K562 cell lysate (20 µg)
Lanes 4, 8 and 12: HEK293 cell lysate (20 µg)
Lanes 1, 2, 3 and 4: Green signal from target – ab11723 observed at 77 kDa
Lanes 5, 6, 7 and 8: Red signal from loading control – ab8245 observed at 37 kDa
Lanes 9, 10, 11 and 12: Merged (red and green) signalab11723 was shown to specifically react with PKC alpha in wild-type HAP1 cells. No band was observed when PKC alpha knockout samples were examined. Wild-type and PKC alpha knockout samples were subjected to SDS-PAGE. ab11723 and ab8245 (loading control to GAPDH) were diluted 1/500 and 1/2000 respectively and incubated overnight at 4ºC. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
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This data was developed using ab11723 the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of Paraffin-embedded human retina tissue labelling PKC alpha with ab11723 at 1/500 dilution, followed by ready to use secondary antibody Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Positive staining on human retina is observed. Counter stained with Hematoxylin. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Goat Anti-Mouse IgG H&L (HRP polymer) ab214879.
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This data was developed using ab11723 the same antibody clone in a different buffer formulation.
This image was generated from the hybridoma version of the product.
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: PKC alpha knockout HAP1 cell lysate (20 µg)
Lane 3: K562 cell lysate (20 µg)
Lane 4: HEK293 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab11723 observed at 77 kDa. Red - loading control, ab8245, observed at 37 kDa.This western blot image is a comparison between ab11723 and a competitor's top cited rabbit polyclonal antibody.
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All lanes : Anti-PKC alpha antibody [133] (ab11723) at 1/1000 dilution
Lane 1 : ab55672
Lane 2 : ab60840
Lane 3 : ab60841
Lane 4 : ab60849
Lane 5 : ab60847
Lane 6 : ab56641
Lane 7 : ab60873
Lane 8 : ab60848
Lane 9 : ab60850
Lane 10 : ab60842
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution
Predicted band size: 76 kDaThis data was developed using ab11723 the same antibody clone in a different buffer formulation.
5% NFDM/TBST
All positive controls 10 ng
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All lanes : Anti-PKC alpha antibody [133] (ab11723) at 1/1000 dilution
Lane 1 : ab55672, 5 ng
Lane 2 : ab60844, 60 ng
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution
Predicted band size: 76 kDaThis data was developed using ab11723 the same antibody clone in a different buffer formulation.
5% NFDM/TBST
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This data was developed using ab11723 the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse retina tissue labelling PKC alpha with ab11723 at 1/500 dilution, followed by ready to use secondary antibody Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Positive staining on mouse retina is observed. Counter stained with Hematoxylin. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Goat Anti-Mouse IgG H&L (HRP polymer) ab214879.
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All lanes : Anti-PKC alpha antibody [133] (ab11723) at 1/1000 dilution
Lane 1 : Human kidney tissue lysate
Lane 2 : Mouse brain tissue lysate
Lane 3 : Mouse spleen tissue lysate
Lane 4 : Rat brain tissue lysate
Lane 5 : Rat spleen tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution
Predicted band size: 76 kDaThis data was developed using ab11723 the same antibody clone in a different buffer formulation.
5% NFDM/TBST
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All lanes : Anti-PKC alpha antibody [133] (ab11723) at 1/1000 dilution
Lane 1 : C6 (rat glial tumor glial cell), whole cell lysate
Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell ), whole cell lysate
Lane 3 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution
Predicted band size: 76 kDaThis data was developed using ab11723 the same antibody clone in a different buffer formulation.
Exposure time
Lane 1: 136 seconds
Lane 2 and 3: 15 secondsFresh lysates were used in lane 2 and 3.
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This data was developed using ab11723 the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat retina tissue labelling PKC alpha with ab11723 at 1/500 dilution, followed by ready to use secondary antibody Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Positive staining on rat retina is observed. Counter stained with Hematoxylin. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Goat Anti-Mouse IgG H&L (HRP polymer) ab214879.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab284403 は論文での使用が確認できていません。