製品名Anti-PKC alpha antibody 
PKC alpha 一次抗体 製品一覧
製品の詳細Mouse monoclonal  to PKC alpha
特異性ab11723 recognises the alpha isoform of PKC. This antibody recognises the sequence PQFVHPILQSAV at the C terminus of PKC alpha.
アプリケーション適用あり: ICC/IF, WB, IHC-Frmore details
種交差性交差種: Mouse, Rat, Human
Full length protein corresponding to PKC alpha.
特記事項This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
バッファーPreservative: 0.09% Sodium azide
Constituent: Tissue culture supernatant
精製度Tissue culture supernatant
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab11723 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. PubMed: 17158168|
|WB||1/10 - 1/500. Predicted molecular weight: 80.6 kDa.Can be blocked with Recombinant human PKC alpha protein (Active) (ab55672).|
|IHC-Fr||1/1 - 1/10.|
機能This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme. May play a role in cell motility by phosphorylating CSPG4.
PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
配列類似性Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 C2 domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain.
細胞内局在Cytoplasm. Cell membrane. Nucleus.
- Information by UniProt
- AAG6 antibody
- Aging associated gene 6 antibody
- aPKC antibody
Lanes 1, 5 and 9: Wild-type HAP1 cell lysate (20 µg)
Lanes 2, 6 and 10: PKC alpha knockout HAP1 cell lysate (20 µg)
Lanes 3, 7 and 11: K562 cell lysate (20 µg)
Lanes 4, 8 and 12: HEK293 cell lysate (20 µg)
Lanes 1, 2, 3 and 4: Green signal from target – ab11723 observed at 77 kDa
Lanes 5, 6, 7 and 8: Red signal from loading control – ab8245 observed at 37 kDa
Lanes 9, 10, 11 and 12: Merged (red and green) signal
ab11723 was shown to specifically react with PKC alpha in wild-type HAP1 cells. No band was observed when PKC alpha knockout samples were examined. Wild-type and PKC alpha knockout samples were subjected to SDS-PAGE. ab11723 and ab8245 (loading control to GAPDH) were diluted 1/500 and 1/2000 respectively and incubated overnight at 4ºC. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: PKC alpha knockout HAP1 cell lysate (20 µg)
Lane 3: K562 cell lysate (20 µg)
Lane 4: HEK293 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab11723 observed at 77 kDa. Red - loading control, ab8245, observed at 37 kDa.
This western blot image is a comparison between ab11723 and a competitor's top cited rabbit polyclonal antibody.
This product has been referenced in:
- De Silva SR et al. Long-term restoration of visual function in end-stage retinal degeneration using subretinal human melanopsin gene therapy. Proc Natl Acad Sci U S A 114:11211-11216 (2017). IHC-P . Read more (PubMed: 28973921) »
- Wang Y et al. The Auxiliary Calcium Channel Subunit a2d4 Is Required for Axonal Elaboration, Synaptic Transmission, and Wiring of Rod Photoreceptors. Neuron 93:1359-1374.e6 (2017). Read more (PubMed: 28262416) »