遺伝子ノックアウト細胞株 検証済

Anti-PKC alpha 抗体 [133] (ab11723)

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製品の概要

  • 製品名

    Anti-PKC alpha antibody [133]
    PKC alpha 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [133] to PKC alpha

  • 由来種

    Mouse

  • 特異性

    ab11723 recognises the alpha isoform of PKC. This antibody recognises the sequence PQFVHPILQSAV at the C terminus of PKC alpha.

  • アプリケーション

    適用あり: ICC/IF, WB, IHC-Frmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Full length protein corresponding to PKC alpha.

  • 特記事項

    This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab11723 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration. PubMed: 17158168
WB 1/10 - 1/500. Predicted molecular weight: 80.6 kDa.Can be blocked with Recombinant human PKC alpha protein (Active) (ab55672).
IHC-Fr 1/1 - 1/10.

ターゲット情報

  • 機能

    This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme. May play a role in cell motility by phosphorylating CSPG4.
    PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.

  • 配列類似性

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 C2 domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.

  • 細胞内局在

    Cytoplasm. Cell membrane. Nucleus.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • AAG6 antibody
    • Aging associated gene 6 antibody
    • aPKC antibody
    • KPCA_HUMAN antibody
    • PKC alpha antibody
    • PKC-A antibody
    • PKC-alpha antibody
    • PKCA antibody
    • PRKACA antibody
    • PRKCA antibody
    • Protein Kinase C alpha antibody
    • Protein kinase C alpha type antibody
    see all

画像

  • Western blot - Anti-PKC alpha antibody [133] (ab11723)
    Western blot - Anti-PKC alpha antibody [133] (ab11723)

    Lanes 1, 5 and 9: Wild-type HAP1 cell lysate (20 µg)
    Lanes 2, 6 and 10: PKC alpha knockout HAP1 cell lysate (20 µg)
    Lanes 3, 7 and 11: K562 cell lysate (20 µg)
    Lanes 4, 8 and 12: HEK293 cell lysate (20 µg)
    Lanes 1, 2, 3 and 4: Green signal from target – ab11723 observed at 77 kDa
    Lanes 5, 6, 7 and 8: Red signal from loading control – ab8245 observed at 37 kDa
    Lanes 9, 10, 11 and 12: Merged (red and green) signal

    ab11723 was shown to specifically react with PKC alpha in wild-type HAP1 cells. No band was observed when PKC alpha knockout samples were examined. Wild-type and PKC alpha knockout samples were subjected to SDS-PAGE. ab11723 and ab8245 (loading control to GAPDH) were diluted 1/500 and 1/2000 respectively and incubated overnight at 4ºC. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-PKC alpha antibody [133] (ab11723)
    Western blot - Anti-PKC alpha antibody [133] (ab11723)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: PKC alpha knockout HAP1 cell lysate (20 µg)
    Lane 3: K562 cell lysate (20 µg)
    Lane 4: HEK293 cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab11723 observed at 77 kDa. Red - loading control, ab8245, observed at 37 kDa.

    This western blot image is a comparison between ab11723 and a competitor's top cited rabbit polyclonal antibody.

参考文献

This product has been referenced in:

  • De Silva SR  et al. Long-term restoration of visual function in end-stage retinal degeneration using subretinal human melanopsin gene therapy. Proc Natl Acad Sci U S A 114:11211-11216 (2017). IHC-P . Read more (PubMed: 28973921) »
  • Wang Y  et al. The Auxiliary Calcium Channel Subunit a2d4 Is Required for Axonal Elaboration, Synaptic Transmission, and Wiring of Rod Photoreceptors. Neuron 93:1359-1374.e6 (2017). Read more (PubMed: 28262416) »
See all 14 Publications for this product

ab11723 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

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Western blot abreview for Anti-PKC alpha antibody [133]

 Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cells)
Specification
HeLa cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
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投稿 Dec 05 2005

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