Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (8)
Sample
Cow Tissue lysate - whole (Testis, Brain, Ovary)
Specification
Testis, Brain, Ovary
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: BSA
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: Cell Signalling, Anti-Rb b-Actin
Host species: Mouse
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Mouse
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Bands
Specific: 70 kDa
Exposure
2 minute(s) and 7 second(s)
Positive control
Testis
Additional data
Additional Notes
Left to right: Ovary, Brain, Testis lysates. Strong banding in testis lysate, faint banding in brain and specific banding in ovary lysate. beta-actin reference band included below
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Stewart Russell
Verified customer
投稿 Feb 18 2014