Application
Western blot
Sample
Mouse Cell lysate - whole cell (Hela #F9 #Ms Testis #PC-12)
Loading amount
30 µg
Specification
Hela #F9 #Ms Testis #PC-12
Gel Running Conditions
Reduced Denaturing (7.5% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Concentration
1 µg/ml
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% Milk, PBS-Tween-20 (0.1%)
Secondary antibody
Name
Non-Abcam antibody was used: Swine anti-rabbit HRP
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
Supersignal west dura
Exposure
4 minute(s) and 0 second(s)
Bands
Specific: 105, 95 kDa Non-specific: 75 kDa
Positive control
Ms testis lysate
Additional data
Additional Notes
Lane order (1-4): Hela #F9 #Ms Testis #PC-12
Strongly immunoreactive bands are uniquely observed in testis lysates one in Mouse F9 cell extract (testis teratocarcinoma) at ~100 kDa and two bands in whole murine testis lysate at ~94 kDa and ~75 kDa. Aceview database describes two Miwi transcripts of consistent size: variant a 99 kDa and variant b 95 kDa. No transcripts corresponding to ~75 kDa species have been described, suggesting that this species is either a cross-reactive band or a degradation product.
Strongly immunoreactive bands are uniquely observed in testis lysates one in Mouse F9 cell extract (testis teratocarcinoma) at ~100 kDa and two bands in whole murine testis lysate at ~94 kDa and ~75 kDa. Aceview database describes two Miwi transcripts of consistent size: variant a 99 kDa and variant b 95 kDa. No transcripts corresponding to ~75 kDa species have been described, suggesting that this species is either a cross-reactive band or a degradation product.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Antibody Solutions
Verified customer
投稿 Mar 05 2009