Anti-Pit1 抗体 (ab272639)
Key features and details
- Rabbit polyclonal to Pit1
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-Pit1 antibody
Pit1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Pit1 -
由来種
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human
-
免疫原
Recombinant fragment corresponding to Human Pit1 aa 1-100.
Database link: P28069 -
ポジティブ･コントロール
- IHC-P: Human pituitary tissue, tonsil tissue, pancreas tissue, liver tissue.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab272639 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| アプリケーション | Species |
|---|---|
| IHC-P |
Human
|
| All applications |
Mouse
Rat
Sheep
Cow
Dog
Turkey
Rhesus monkey
|
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 特記事項 |
|---|
|
IHC-P
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
-
機能
Transcription factor involved in the specification of the lactotrope, somatotrope, and thyrotrope phenotypes in the developing anterior pituitary. Activates growth hormone and prolactin genes. Specifically binds to the consensus sequence 5'-TAAAT-3'. -
関連疾患
Defects in POU1F1 are the cause of pituitary hormone deficiency combined type 1 (CPHD1) [MIM:613038]. CPHD is characterized by impaired production of growth hormone (GH) and one or more of the other five anterior pituitary hormones. -
配列類似性
Belongs to the POU transcription factor family. Class-1 subfamily.
Contains 1 homeobox DNA-binding domain.
Contains 1 POU-specific domain. -
細胞内局在
Nucleus. - Information by UniProt
-
参照データベース
- Entrez Gene: 282315 Cow
- Entrez Gene: 403753 Dog
- Entrez Gene: 5449 Human
- Entrez Gene: 18736 Mouse
- Entrez Gene: 25517 Rat
- Entrez Gene: 397325 Sheep
- Omim: 173110 Human
- SwissProt: P28069 Human
see all -
別名
- Dwarf antibody
- GHF 1 antibody
- GHF 1A antibody
see all
画像
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pit1 antibody (ab272639)
Paraffin-embedded formalin-fixed Human pituitary tissue stained for Pit1 using ab272639 at 1/2500 in immunohistochemical analysis.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pit1 antibody (ab272639)Paraffin-embedded formalin-fixed Human tonsil tissue stained for Pit1 using ab272639 at 1/2500 in immunohistochemical analysis. Shows no expression as expected.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pit1 antibody (ab272639)Paraffin-embedded formalin-fixed Human pancreas tissue stained for Pit1 using ab272639 at 1/2500 in immunohistochemical analysis. Shows no expression as expected.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pit1 antibody (ab272639)Paraffin-embedded formalin-fixed Human liver tissue stained for Pit1 using ab272639 at 1/2500 in immunohistochemical analysis. Shows no expression as expected.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab272639 は論文での使用が確認できていません。