Anti-Pirh2 抗体 [EPR18553] (ab189907)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18553] to Pirh2
- Suitable for: ICC/IF, IP, WB
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Pirh2 antibody [EPR18553]
Pirh2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR18553] to Pirh2 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IP, WBmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: HeLa, HepG2, Daudi, HEK-293, HCT 116, LNCaP, RAW 264.7 and NIH/3T3 whole cell lysates; Human fetal heart, fetal kidney and fetal spleen lysates; Mouse kidney and spleen lysates. ICC/IF: HeLa and HEK-293 cells. IP: HeLa whole cell lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR18553 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab189907の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
1/500.
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IP |
1/50.
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WB |
1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).
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特記事項 |
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ICC/IF
1/500. |
IP
1/50. |
WB
1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa). |
ターゲット情報
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機能
Mediates E3-dependent ubiquitination and proteasomal degradation of target proteins, including p53/TP53, HDAC1 and CDKN1B. Preferentially acts on tetrameric p53/TP53. Contributes to the regulation of CDKN1B and p53/TP53 levels, and thereby contributes to the regulation of the cell cycle progression. Increases AR transcription factor activity. -
パスウェイ
Protein modification; protein ubiquitination. -
配列類似性
Contains 1 CHY-type zinc finger.
Contains 1 CTCHY-type zinc finger.
Contains 1 RING-type zinc finger. -
翻訳後修飾
Subject to ubiquitination and proteasomal degradation. Interaction with PLAGL2 or KAT5 enhances protein stability. -
細胞内局在
Nucleus. Nucleus speckle. Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 25898 Human
- Entrez Gene: 68098 Mouse
- Omim: 607680 Human
- SwissProt: Q96PM5 Human
- SwissProt: Q9CR50 Mouse
- Unigene: 48297 Human
- Unigene: 159453 Mouse
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別名
- Androgen receptor N terminal interacting protein antibody
- Androgen receptor N-terminal-interacting protein antibody
- ARNIP antibody
see all
画像
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All lanes : Anti-Pirh2 antibody [EPR18553] (ab189907) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : RCHY1 knockout HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : Daudi cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 30 kDaLanes 1-4: Merged signal (red and green). Green - ab189907 observed at 30 kDa. Red - loading control ab8245 observed at 37 kDa.
ab189907 Anti-Pirh2 antibody [EPR18553] was shown to specifically react with RCHY1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265478 (knockout cell lysate ab258171) was used. Wild-type and RCHY1 knockout samples were subjected to SDS-PAGE. ab189907 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling Pirh2 with ab189907 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing nuclear and cytoplasmic staining on HeLa cell line.
The nuclear counterstain is DAPI (blue).
Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows:-
-ve control 1: ab189907 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293 (Human epithelial cells from embryonic kidney) cells labeling Pirh2 with ab189907 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing nuclear and cytoplasmic staining on HEK-293 cell line.
The nuclear counterstain is DAPI (blue).
Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows:-
-ve control 1: ab189907 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.
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All lanes : Anti-Pirh2 antibody [EPR18553] (ab189907) at 1/1000 dilution
Lane 1 : HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate
Lane 2 : HCT 116 (Human colorectal carcinoma cell line) whole cell lysate
Lane 3 : LNCaP (Human prostate cancer cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 30 kDa
Observed band size: 30 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-Pirh2 antibody [EPR18553] (ab189907) at 1/1000 dilution
Lanes 1 & 3 : Human fetal heart lysate
Lane 2 : Human fetal kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 30 kDa
Observed band size: 30 kDa
Exposure time: 30 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-Pirh2 antibody [EPR18553] (ab189907) at 1/1000 dilution
Lane 1 : Mouse kidney lysate
Lane 2 : Mouse spleen lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 30 kDa
Observed band size: 30 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-Pirh2 antibody [EPR18553] (ab189907) at 1/1000 dilution
Lane 1 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
Lane 2 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 30 kDa
Observed band size: 30 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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Pirh2 was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with ab189907 at 1/50 dilution.
Western blot was performed from the immunoprecipitate using ab189907 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: HeLa whole cell lysate 10ug (Input).
Lane 2: ab189907 IP in HeLa whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab189907 in HeLa whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (2)
ab189907 は 2 報の論文で使用されています。
- Zhang X et al. IMP3 accelerates the progression of prostate cancer through inhibiting PTEN expression in a SMURF1-dependent way. J Exp Clin Cancer Res 39:190 (2020). PubMed: 32938489
- Yu S et al. Pyrrolidine Dithiocarbamate Facilitates Arsenic Trioxide Against Pancreatic Cancer via Perturbing Ubiquitin-Proteasome Pathway. Cancer Manag Res 12:13149-13159 (2020). PubMed: 33376406