製品の概要

  • 製品名
    Anti-PIP2 antibody
    PIP2 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to PIP2
  • 由来種
    Mouse
  • 特異性
    This antibody cross reacts with Phosphatidic acid (<5.0%).
  • アプリケーション
    適用あり: ICC/IFmore details
  • 免疫原

    Phosphatidylinositol-4,5-biphosphate (PIP2) from bovine spinal cord

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab2335 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration.

TBS buffer is ideal.

ターゲット情報

  • 関連性
    Phosphatidylinositol 4,5-biphosphate (PIP2) is a membrane phospholipid that has been implicated in a variety of cellular processes, including synaptic vesicle recycling and signal transduction pathways. PLCD4 hydrolyzes PIP2 to generate 2 second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3).

画像

  • Immunocytochemistry/ Immunofluorescence - Anti-PIP2 antibody (ab2335)
    Immunocytochemistry/ Immunofluorescence - Anti-PIP2 antibody (ab2335)Image from Brzeska H et al., J Biol Chem. 2012 Apr 27;287(18):14923-36. Epub 2012 Feb 24. Fig 1.; doi: 10.1074/jbc.M111.318667; April 27, 2012, The Journal of Biological Chemistry, 287, 14923-14936.
    ab2335, staining PIP2 in non-polarized (left) and polarized (right) AX3 cells by Immunocytochemisry/ Immunofluorescence.

    Cells were fixed with formaldehyde, glutaraldehyde, and 0.01% Triton X-100 in PBS for 10 min. Samples were incubated with primary antibody at 1/100 dilution.

参考文献

This product has been referenced in:
  • Wahl S  et al. The Disease Protein Tulp1 Is Essential for Periactive Zone Endocytosis in Photoreceptor Ribbon Synapses. J Neurosci 36:2473-93 (2016). Read more (PubMed: 26911694) »
  • Li J  et al. Actin dynamics is rapidly regulated by the PTEN and PIP2 signaling pathways leading to myocyte hypertrophy. Am J Physiol Heart Circ Physiol 307:H1618-25 (2014). Read more (PubMed: 25260617) »

See all 5 Publications for this product

ab2335 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

レビューと Q&A

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6 reviews or Q&A

10
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I am wondering if ab2335 is the same clone (KT 10) as was developed by Fukami, et al in the 1988 PNAS paper (Vol. 85 pp.9057-61). If not, any information about the development of this specific clone would be much appreciated.
I am trying to l...

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Thank you for contacting Abcam

I have talked to the supplier of this antibody and I am sorry but they do not have any detailed clone information that I can share and cannot confirm that this antibody is the same as the one published in the pa...

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Does this antibody cross-react with PIP3? What protocols were used to test this antibody in ELISA and ICC?

Thank you very much for your call last week, and I'm very sorry for the delay getting back to you.

I've just heard from the lab, and unfortunately ab2335 has not been tested for cross-reactivity with PIP3. The original clone was produced and ...

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What is the cross reactivity of this antibody with other PtdIns?

Thank you for your enquiry. The cross reactivity of this antibody with other PtdIns is stated below. PIP2 100%; Phosphatidic acid <5.0%; PIP 0.1%; Phosphatidyl inositol <0.2%; Phosphatidyl choline <0.2%; Phosphatidyl serine <0.2%; Phosphatidyl...

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Is ab2335 clone 2C11?

Thank you for your enquiry. No ab2335 is not clone 2C11. Please do not hesitate to contact me should you require further assistance.

Has ab2335 been tested yet for immunocytochemistry?

To our knowledge, this antibody has yet to be tested in ICC. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get on and in return we will forward a rewar...

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According to your datasheet, ab2335's tested applications are ELISA and radioimmunoassay. Is there a published ELISA potocol using ab2335? If so, what is the reference? Also, is there information on the cross reactivity to other phosphatidylinositol...

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Thank you for your enquiry, Here are two publications citing the use of this antibody: RIA: Fukami, K., Takenawa, T. (1989) Quantitative Changes in Polysphophoinositides 1,2-diacyglycerol and Inositol 1,4,5-Trisphosphate by Platelet-Derived Growth...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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