Anti-PIP2 抗体 (ab2335)
製品の概要
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製品名
Anti-PIP2 antibody
PIP2 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal to PIP2 -
由来種
Mouse -
特異性
This antibody cross reacts with Phosphatidic acid (<5.0%). -
アプリケーション
適用あり: ICC/IFmore details -
免疫原
Phosphatidylinositol-4,5-biphosphate (PIP2) from bovine spinal cord
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.1% Sodium azide
Constituents: PBS, 10% Fetal calf serum -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
アイソタイプ
IgG2b -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
Our Abpromise guarantee covers the use of ab2335 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
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ICC/IF | Use at an assay dependent concentration. TBS buffer is ideal. |
ターゲット情報
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関連性
Phosphatidylinositol 4,5-biphosphate (PIP2) is a membrane phospholipid that has been implicated in a variety of cellular processes, including synaptic vesicle recycling and signal transduction pathways. PLCD4 hydrolyzes PIP2 to generate 2 second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3).
画像
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Immunocytochemistry/ Immunofluorescence - Anti-PIP2 antibody (ab2335)Image from Brzeska H et al., J Biol Chem. 2012 Apr 27;287(18):14923-36. Epub 2012 Feb 24. Fig 1.; doi: 10.1074/jbc.M111.318667; April 27, 2012, The Journal of Biological Chemistry, 287, 14923-14936.ab2335, staining PIP2 in non-polarized (left) and polarized (right) AX3 cells by Immunocytochemisry/ Immunofluorescence.
Cells were fixed with formaldehyde, glutaraldehyde, and 0.01% Triton X-100 in PBS for 10 min. Samples were incubated with primary antibody at 1/100 dilution.
参考文献
This product has been referenced in:
- Suzuki H et al. Regulation of MC3T3-E1 differentiation by actin cytoskeleton through lipid mediators reflecting the cell differentiation stage. Biochem Biophys Res Commun 514:393-400 (2019). Read more (PubMed: 31047639) »
- Roman-Garcia S et al. Distinct Roles for Bruton's Tyrosine Kinase in B Cell Immune Synapse Formation. Front Immunol 9:2027 (2018). Read more (PubMed: 30237801) »