Anti-PIP2 抗体 (ab2335)

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Key features and details

  • Mouse monoclonal to PIP2
  • Suitable for: ICC/IF
  • Isotype: IgG2b

製品の概要

  • 製品名

    Anti-PIP2 antibody
    PIP2 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal to PIP2

  • 由来種

    Mouse

  • 特異性

    This antibody cross reacts with Phosphatidic acid (<5.0%).

  • アプリケーション

    適用あり: ICC/IFmore details

  • 免疫原

    Phosphatidylinositol-4,5-biphosphate (PIP2) from bovine spinal cord

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab2335 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration.

TBS buffer is ideal.

ターゲット情報

  • 関連性

    Phosphatidylinositol 4,5-biphosphate (PIP2) is a membrane phospholipid that has been implicated in a variety of cellular processes, including synaptic vesicle recycling and signal transduction pathways. PLCD4 hydrolyzes PIP2 to generate 2 second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3).

画像

  • Immunocytochemistry/ Immunofluorescence - Anti-PIP2 antibody (ab2335)
    Immunocytochemistry/ Immunofluorescence - Anti-PIP2 antibody (ab2335)Image from Brzeska H et al., J Biol Chem. 2012 Apr 27;287(18):14923-36. Epub 2012 Feb 24. Fig 1.; doi: 10.1074/jbc.M111.318667; April 27, 2012, The Journal of Biological Chemistry, 287, 14923-14936.
    ab2335, staining PIP2 in non-polarized (left) and polarized (right) AX3 cells by Immunocytochemisry/ Immunofluorescence.

    Cells were fixed with formaldehyde, glutaraldehyde, and 0.01% Triton X-100 in PBS for 10 min. Samples were incubated with primary antibody at 1/100 dilution.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献

ab2335 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab2335 は 9 報の論文で使用されています。

  • Suzuki H  et al. Regulation of MC3T3-E1 differentiation by actin cytoskeleton through lipid mediators reflecting the cell differentiation stage. Biochem Biophys Res Commun 514:393-400 (2019). PubMed: 31047639
  • Roman-Garcia S  et al. Distinct Roles for Bruton's Tyrosine Kinase in B Cell Immune Synapse Formation. Front Immunol 9:2027 (2018). PubMed: 30237801
  • Mkrtschjan MA  et al. Lipid signaling affects primary fibroblast collective migration and anchorage in response to stiffness and microtopography. J Cell Physiol 233:3672-3683 (2018). PubMed: 29034471
  • Wahl S  et al. The Disease Protein Tulp1 Is Essential for Periactive Zone Endocytosis in Photoreceptor Ribbon Synapses. J Neurosci 36:2473-93 (2016). PubMed: 26911694
  • Aoun J  et al. Anoctamin 6 Contributes to Cl- Secretion in Accessory Cholera Enterotoxin (Ace)-stimulated Diarrhea: AN ESSENTIAL ROLE FOR PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE (PIP2) SIGNALING IN CHOLERA. J Biol Chem 291:26816-26836 (2016). PubMed: 27799301
  • Li J  et al. Actin dynamics is rapidly regulated by the PTEN and PIP2 signaling pathways leading to myocyte hypertrophy. Am J Physiol Heart Circ Physiol 307:H1618-25 (2014). PubMed: 25260617
  • Brzeska H  et al. Molecular basis of dynamic relocalization of Dictyostelium myosin IB. J Biol Chem 287:14923-36 (2012). ICC/IF . PubMed: 22367211
  • Goldston AM  et al. Exposure to host ligands correlates with colocalization of Gal/GalNAc lectin subunits in lipid rafts and phosphatidylinositol (4,5)-bisphosphate signaling in Entamoeba histolytica. Eukaryot Cell 11:743-51 (2012). PubMed: 22505337
  • Ehrlich LS  et al. Activation of the inositol (1,4,5)-triphosphate calcium gate receptor is required for HIV-1 Gag release. J Virol 84:6438-51 (2010). PubMed: 20427533

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1-6 of 6 Abreviews or Q&A

Question


I am wondering if ab2335 is the same clone (KT 10) as was developed by Fukami, et al in the 1988 PNAS paper (Vol. 85 pp.9057-61). If not, any information about the development of this specific clone would be much appreciated.
I am trying to look for PIP2 in Megakaryocytes (hematopoeitic cells) and am looking for a good ab for IF.

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Answer

Thank you for contacting Abcam

I have talked to the supplier of this antibody and I am sorry but they do not have any detailed clone information that I can share and cannot confirm that this antibody is the same as the one published in the paper indicated.
Sorry that I cannot be of more help in this matter, but if there is anything else I can help you with, please elt me know.

Free RabMab with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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Question

Does this antibody cross-react with PIP3? What protocols were used to test this antibody in ELISA and ICC?

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Answer

Thank you very much for your call last week, and I'm very sorry for the delay getting back to you.

I've just heard from the lab, and unfortunately ab2335 has not been tested for cross-reactivity with PIP3. The original clone was produced and tested by an outside lab, and we don't have the full ELISA or ICC protocols at this time.

I apologize that we don't have more information about this antibody, but if you have further questions or comments, or if there is anything else that we can do for you, please let me know and I'll be happy to help.

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Question

What is the cross reactivity of this antibody with other PtdIns?

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Answer

Thank you for your enquiry. The cross reactivity of this antibody with other PtdIns is stated below. PIP2 100%; Phosphatidic acid <5.0%; PIP 0.1%; Phosphatidyl inositol <0.2%; Phosphatidyl choline <0.2%; Phosphatidyl serine <0.2%; Phosphatidyl ethanolamine <0.2%; Phosphatidyl glycerol <0.2%; Cardiolipin <0.2%; Cholesterol <0.2%; Diacylglycerol <0.2%

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Question

Is ab2335 clone 2C11?

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Answer

Thank you for your enquiry. No ab2335 is not clone 2C11. Please do not hesitate to contact me should you require further assistance.

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Question

Has ab2335 been tested yet for immunocytochemistry?

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Answer

To our knowledge, this antibody has yet to be tested in ICC. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get on and in return we will forward a reward of your choice, typically an Amazon gift voucher. If you have any additional questions, please contact us again.

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Question

According to your datasheet, ab2335's tested applications are ELISA and radioimmunoassay. Is there a published ELISA potocol using ab2335? If so, what is the reference? Also, is there information on the cross reactivity to other phosphatidylinositols? regards,

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Answer

Thank you for your enquiry, Here are two publications citing the use of this antibody: RIA: Fukami, K., Takenawa, T. (1989) Quantitative Changes in Polysphophoinositides 1,2-diacyglycerol and Inositol 1,4,5-Trisphosphate by Platelet-Derived Growth Factor and Prostaglandin F2a. The Journal of Biological Chemistry 264(25):1485-1489. ELISA: Imai, K., et al. (1999) The Pleckstrin Homology Domain of the Wiskott-Aldrich Syndrome Protein Is Involved in the Organization of Actin Cytoskeleton. Clinical Immunology 92(2):128-137. We do not have any information regarding the cross reactivity to other phosphatidylinositols, my apologies for the inconvenience,

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