Anti-PINK1 抗体 (ab23707)
Key features and details
- Rabbit polyclonal to PINK1
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-PINK1 antibody
PINK1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PINK1 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide:
LVRALLQREA SKRPSARVAA N
, corresponding to amino acids 484-504 of Human PINK1. -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab23707の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | (2) |
1/100 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB | (15) |
1/200 - 1/1000. Detects a band of approximately 66 kDa.Can be blocked with Human PINK1 peptide (ab30903). Detects a band of approximately 66 kDa. Can be blocked with PINK1 peptide (484-504) (ab30903).
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| 特記事項 |
|---|
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IHC-P
1/100 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
1/200 - 1/1000. Detects a band of approximately 66 kDa.Can be blocked with Human PINK1 peptide (ab30903). Detects a band of approximately 66 kDa. Can be blocked with PINK1 peptide (484-504) (ab30903). |
ターゲット情報
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機能
Protects against mitochondrial dysfunction during cellular stress, potentially by phosphorylating mitochondrial proteins. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). It is necessary for PARK2 recruitement to dysfunctional mitochondria to initiate their degradation. -
組織特異性
Highly expressed in heart, skeletal muscle and testis, and at lower levels in brain, placenta, liver, kidney, pancreas, prostate, ovary and small intestine. Present in the embryonic testis from an early stage of development. -
関連疾患
Defects in PINK1 are the cause of Parkinson disease type 6 (PARK6) [MIM:605909]. A neurodegenerative disorder characterized by parkinsonian signs such as rigidity, resting tremor and bradykinesia. A subset of patients manifest additional symptoms including hyperreflexia, autonomic instability, dementia and psychiatric disturbances. Symptoms show diurnal fluctuation and can improve after sleep. -
配列類似性
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Contains 1 protein kinase domain. -
翻訳後修飾
Autophosphorylated. -
細胞内局在
Mitochondrion outer membrane. Cytoplasm > cytosol. - Information by UniProt
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参照データベース
- Entrez Gene: 65018 Human
- Entrez Gene: 68943 Mouse
- NCBI: 14165272 Human
- Omim: 608309 Human
- SwissProt: Q9BXM7 Human
- SwissProt: Q99MQ3 Mouse
- Unigene: 389171 Human
- Unigene: 18539 Mouse
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別名
- BRPK antibody
- FLJ27236 antibody
- mitochondrial antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PINK1 antibody (ab23707)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling PINK1 with ab23707 at 1/200 dilution. Slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen (DAB). Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Western blot - Anti-PINK1 antibody (ab23707)Anti-PINK1 antibody (ab23707) at 4 µg/ml + Murine liver 100,000 x g pellet at 30 µg
Observed band size: 66 kDa why is the actual band size different from the predicted?
Additional bands at: 33 kDa (possible cleavage fragment) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PINK1 antibody (ab23707)This image is courtesy of an Abreview submitted by Mr. Allan Wang.Immunohistochemistry (Formaldehyde-fixed paraffin-embedded sections) analysis of human colon adenocarcinoma tissues labelling PINK1 with ab23707 at 1/100 dilution (28 minutes at 37°C). Heat mediated antigen retrieval was performed using a buffer at pH 8.5. An HRP-conjugated Goat Anti-rabbit polyclonal was used as the secondary antibody.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (129)
ab23707 は 129 報の論文で使用されています。
- Kim YJ et al. BCL2 Interacting Protein 3-like/NIX-mediated Mitophagy Plays an Important Role in the Process of Age-related Hearing Loss. Neuroscience 455:39-51 (2021). PubMed: 33346118
- Sass F et al. TFEB deficiency attenuates mitochondrial degradation upon brown adipose tissue whitening at thermoneutrality. Mol Metab 47:101173 (2021). PubMed: 33516944
- Wang M et al. S-Nitroso-L-Cysteine Ameliorated Pulmonary Hypertension in the MCT-Induced Rats through Anti-ROS and Anti-Inflammatory Pathways. Oxid Med Cell Longev 2021:6621232 (2021). PubMed: 33574976
- Roca-Agujetas V et al. Cholesterol alters mitophagy by impairing optineurin recruitment and lysosomal clearance in Alzheimer's disease. Mol Neurodegener 16:15 (2021). PubMed: 33685483
- Hu J et al. Elevated Lactate by High-Intensity Interval Training Regulates the Hippocampal BDNF Expression and the Mitochondrial Quality Control System. Front Physiol 12:629914 (2021). PubMed: 33716776