Anti-PINCH-2 抗体 (ab272666)
Key features and details
- Rabbit polyclonal to PINCH-2
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-PINCH-2 antibody -
製品の詳細
Rabbit polyclonal to PINCH-2 -
由来種
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human
-
免疫原
Recombinant fragment corresponding to Human PINCH-2 aa 300-400.
Database link: Q7Z4I7 -
ポジティブ･コントロール
- WB: Human liver and tonsil tissue. IHC-P: Human prostate, kidney and skin tissue. IF: BJ cells.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab272666 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| アプリケーション | Species |
|---|---|
| ICC/IF |
Human
|
| IHC-P |
Human
|
| WB |
Human
|
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
|
|
| WB |
Use a concentration of 0.04 - 0.4 µg/ml.
|
|
| IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 特記事項 |
|---|
|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
|
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Competes with LIMS1 for binding to ILK. Plays a role in modulating cell spreading and migration. -
配列類似性
Contains 5 LIM zinc-binding domains. -
細胞内局在
Nucleus. Cell junction > focal adhesion. Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 55679 Human
- Omim: 607908 Human
- SwissProt: Q7Z4I7 Human
- Unigene: 469881 Human
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別名
- FLJ10044 antibody
- ILK binding protein antibody
- LIM and senescent cell antigen like domains 2 antibody
see all
画像
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Western blot - Anti-PINCH-2 antibody (ab272666)Lanes 2-6 : Anti-PINCH-2 antibody (ab272666) at 0.4 µg/ml
Lane 1 : Molecular Weight ladder
Lane 2 : RT4 cells (Human urinary bladder cancer cell line)
Lane 3 : U-251 MG cells (Human brain glioma cell line)
Lane 4 : Human plasma (IgG/HSA depleted)
Lane 5 : Human liver tissue
Lane 6 : Human tonsil tissue -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PINCH-2 antibody (ab272666)Paraffin-embedded formalin-fixed human prostate tissue stained for PINCH-2 using ab272666 at 1/500 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-PINCH-2 antibody (ab272666)PFA fixed, Triton X-100 permeablized BJ cells (human skin fibroblast cell line) labeling PINCH-2 (green) using ab272666 at 2 ug/ml in ICC/IF analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PINCH-2 antibody (ab272666)Paraffin-embedded formalin-fixed human kidney tissue stained for PINCH-2 using ab272666 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PINCH-2 antibody (ab272666)Paraffin-embedded formalin-fixed human skin tissue stained for PINCH-2 using ab272666 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PINCH-2 antibody (ab272666)Paraffin-embedded formalin-fixed human pancreas tissue stained for PINCH-2 using ab272666 at 1/500 dilution in immunohistochemical analysis. Shows low expression as expected.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
参考文献 (0)
ab272666 は論文での使用が確認できていません。
