Anti-Pin1 抗体 (ab12107)
Key features and details
- Rabbit polyclonal to Pin1
- Suitable for: WB
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Pin1 antibody
Pin1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Pin1 -
由来種
Rabbit -
特異性
ab12107 detects human Pin 1 at ~20kDa. ab12107 also detects a band at ~23kDa, which is not attenuated by blocking peptide ab12397; the identity of this band is unknown. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human, Recombinant fragment
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide corresponding to Human Pin1 aa 50-150 (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab12397) -
ポジティブ･コントロール
- This antibody gave a positive result with Pin1 recombinant protein
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab12107の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 2 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 18.2 kDa).
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| 特記事項 |
|---|
|
WB
Use a concentration of 2 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 18.2 kDa). |
ターゲット情報
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機能
Essential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Displays a preference for an acidic residue N-terminal to the isomerized proline bond. Catalyzing pSer/Thr-Pro cis/trans isomerizations. -
配列類似性
Contains 1 PpiC domain.
Contains 1 WW domain. -
ドメイン
The WW domain is required for the interaction with STIL and KIF20B. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 5300 Human
- Entrez Gene: 23988 Mouse
- Entrez Gene: 298696 Rat
- Omim: 601052 Human
- SwissProt: Q13526 Human
- SwissProt: Q9QUR7 Mouse
- Unigene: 465849 Human
- Unigene: 7906 Mouse
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別名
- DOD antibody
- DODO, Drosophila, homolog of antibody
- FLJ40239 antibody
see all
画像
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Western blot - Anti-Pin1 antibody (ab12107)Lane 1 : Marker
Lanes 2-3 : Anti-Pin1 antibody (ab12107) at 1 µg/ml
Lane 2 : pin1 Recombinant 0.1ug
Lane 3 : pin1 Recombinant 0.5ug
Secondary
Lanes 2-3 : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 18.2 kDa
Observed band size: ~34 kDa why is the actual band size different from the predicted?
The observed band size is consistent with the expected size of the Pin1 partial recombinant protein used (amino acids 64 - 163 of the human Pin1 sequence, with GST).
データシートおよび資料
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Datasheet download
参考文献 (2)
ab12107 は 2 報の論文で使用されています。
- Bhaskaran N et al. Fbw7a and Fbw7? collaborate to shuttle cyclin E1 into the nucleolus for multiubiquitylation. Mol Cell Biol 33:85-97 (2013). Human . PubMed: 23109421
- Lv L et al. Inhibition of peptidyl-prolyl cis/trans isomerase Pin1 induces cell cycle arrest and apoptosis in vascular smooth muscle cells. Apoptosis 15:41-54 (2010). WB ; Human . PubMed: 19904610
