Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6988] to PIM2 - BSA and Azide free
- Suitable for: ICC/IF, IP, WB
- Reacts with: Mouse, Rat, Human
製品名Anti-PIM2 antibody [EPR6988] - BSA and Azide free
PIM2 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EPR6988] to PIM2 - BSA and Azide free
アプリケーション適用あり: ICC/IF, IP, WBmore details
適用なし: Flow Cyt or IHC-P
種交差性交差種: Mouse, Rat, Human
Synthetic peptide within Human PIM2 aa 250-350. The exact sequence is proprietary.
Ab248344 is the carrier-free version of ab129193. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab248344 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
保存方法Shipped at 4°C. Store at +4°C. Do Not Freeze.
解離定数（KD 値）KD = 6.10 x 10 -11 M Learn more about KD
Concentration information loading...
精製度Protein A purified
Our Abpromise guarantee covers the use of ab248344 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 34-40 kDa (predicted molecular weight: 34 kDa).|
機能Promotes cell survival in response to a variety of proliferative signals via positive regulation of the I-kappaB kinase/NF-kappaB cascade; this process requires phosphorylation of MAP3K8/COT. Prevents apoptosis induced by growth factor withdrawal via inhibition of caspase-3 activation, and via phosphorylation of pro-apoptotic proteins. Inhibits BAD-induced cell death via phosphorylation of BAD. PIM2-mediated cell survival is glucose-dependent but independent of several AKT regulators such as PI3K, HSP-90 and TOR, indicating that PIM2 and PI3K/AKT/TOR function via distinct pathways. Involved in the positive regulation of chondrocyte survival and autophagy in the epiphyseal growth plate.
組織特異性Highly expressed in hematopoietic tissues, in leukemic and lymphoma cell lines, testis, small intestine, colon and colorectal adenocarcinoma cells. Weakly expressed in normal liver, but highly expressed in hepatocellular carcinoma tissues.
配列類似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PIM subfamily.
Contains 1 protein kinase domain.
- Information by UniProt
- OTTHUMP00000025825 antibody
- Pim 2 oncogene antibody
- Pim 2h antibody
All lanes : Anti-PIM2 antibody [EPR6988] (ab129193) at 1/1000 dilution
Lane 1 : Jurkat cell lysates
Lane 2 : K562 cell lysates
Lane 3 : Raji cell lysates
Lane 4 : HL60 cell lysates
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 34 kDa
This data was developed using ab129193, the same antibody clone in a different buffer formulation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.