Anti-PIGC 抗体 (ab272617)

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Western blot - Anti-PIGC antibody (ab272617)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIGC antibody (ab272617)

Key features and details

  • Rabbit polyclonal to PIGC
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-PIGC antibody

  • 製品の詳細

    Rabbit polyclonal to PIGC

  • 由来種

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • 免疫原

    Recombinant fragment corresponding to Human PIGC aa 250-350.
    Database link: Q92535

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • ポジティブ･コントロール

    • WB: SK-MEL-30. IHC-P: Human colon tissue.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab272617 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
IHC-P
Human
WB
Human
All applications
Rat
Cow
アプリケーション Abreviews 特記事項
IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
Use a concentration of 0.04 - 0.4 µg/ml.
特記事項
IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
Use a concentration of 0.04 - 0.4 µg/ml.

ターゲット情報

  • 関連性

    This gene encodes an endoplasmic reticulum associated protein that is involved in glycosylphosphatidylinositol (GPI) lipid anchor biosynthesis. The GPI lipid anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. The encoded protein is one subunit of the GPI N-acetylglucosaminyl (GlcNAc) transferase that transfers GlcNAc to phosphatidylinositol (PI) on the cytoplasmic side of the endoplasmic reticulum. Two alternatively spliced transcripts that encode the same protein have been found for this gene. A pseudogene on chromosome 11 has also been characterized.

  • 細胞内局在

    Endoplasmic reticulum membrane; Multi-pass membrane protein

  • 参照データベース

    • 別名

      • GPI2 antibody
      • Phosphatidylinositol glycan biosynthesis class C protein antibody
      • Phosphatidylinositol N acetylglucosaminyltransferase subunit C antibody

    画像

    • Western blot - Anti-PIGC antibody (ab272617)
      Western blot - Anti-PIGC antibody (ab272617)
      Lane 2 : Anti-PIGC antibody (ab272617) at 0.4 µg/ml

      Lane 1 : Molecular weight ladder
      Lane 2 : Human cell line SK-MEL-30
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIGC antibody (ab272617)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIGC antibody (ab272617)

      Paraffin-embedded formalin-fixed Human colon tissue stained for PIGC using ab272617 at 1/50 in immunohistochemical analysis.

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab272617 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab272617 は論文での使用が確認できていません。

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