Pig Prothrombin ELISA Kit (ab108890)
Key features and details
- Sensitivity: 0.3 ng/ml
- Range: 0.391 ng/ml - 400 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Pig
製品の概要
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製品名
Pig Prothrombin ELISA Kit
Prothrombin キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Overall 4.8% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Overall 9.6% -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
= 0.3 ng/ml -
検出範囲
0.391 ng/ml - 400 ng/ml -
添加回収試験
98 %
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全工程の試験時間
4h 00m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Pig -
製品の概要
Abcam’s Prothrombin Pig in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of prothrombin levels in plasma, serum and cell culture supernatants.
A Prothrombin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Prothrombin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Prothrombin captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 50X Biotinylated Pig Prothrombin Antibody 1 x 120µl 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent M Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml Chromogen Substrate 1 x 8ml Prothrombin Microplate (12 x 8 well strips) 1 unit Prothrombin Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 12ml -
研究分野
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機能
Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing. -
組織特異性
Expressed by the liver and secreted in plasma. -
関連疾患
Defects in F2 are the cause of factor II deficiency (FA2D) [MIM:613679]. It is a very rare blood coagulation disorder characterized by mucocutaneous bleeding symptoms. The severity of the bleeding manifestations correlates with blood factor II levels.
Genetic variations in F2 may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.
Defects in F2 are a cause of susceptibility to thrombosis (THR) [MIM:188050]. It is a multifactorial disorder of hemostasis characterized by abnormal platelet aggregation in response to various agents and recurrent thrombi formation. Note=A common genetic variation in the 3-prime untranslated region of the prothrombin gene is associated with elevated plasma prothrombin levels and an increased risk of venous thrombosis. -
配列類似性
Belongs to the peptidase S1 family.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 2 kringle domains.
Contains 1 peptidase S1 domain. -
翻訳後修飾
The gamma-carboxyglutamyl residues, which bind calcium ions, result from the carboxylation of glutamyl residues by a microsomal enzyme, the vitamin K-dependent carboxylase. The modified residues are necessary for the calcium-dependent interaction with a negatively charged phospholipid surface, which is essential for the conversion of prothrombin to thrombin. -
細胞内局在
Secreted > extracellular space. - Information by UniProt
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別名
- Coagulation factor II
- coagulation factor II (thrombin)
- F2
see all -
参照データベース
画像
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Typical Standard Curve
Representative Standard Curve using ab108890
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab108890 は論文での使用が確認できていません。