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  1. Link

    pig-il-6-elisa-kit-ab100755.pdf

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Immunology Innate Immunity Macrophage / Inflamm.
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Pig IL-6 ELISA Kit (ab100755)

  • Datasheet
  • SDS
  • Protocol Booklet
Reviews (1)Q&A (6)References (14)

Product price, shipping and contact information

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Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Representative Standard Curve

    Key features and details

    • Sensitivity: 45 pg/ml
    • Range: 40.96 pg/ml - 10000 pg/ml
    • Sample type: Cell culture supernatant, Plasma, Serum
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Pig

    こちらの製品もご検討ください

    ELISA
    Product image
    Pig IL-1 beta ELISA Kit (ab100754)
    分離
    Product image
    Lipid Extraction Kit (Chloroform Free) (ab211044)
    アッセイ
    Product image
    Cholesterol/ Cholesteryl Ester Assay Kit - Quantitation (ab65359)

    関連製品

    製品の概要

    • 製品名

      Pig IL-6 ELISA Kit
      IL-6 キット 製品一覧
    • 検出方法

      Colorimetric
    • サンプルの種類

      Cell culture supernatant, Serum, Plasma
    • アッセイタイプ

      Sandwich (quantitative)
    • 検出感度

      < 45 pg/ml
    • 検出範囲

      40.96 pg/ml - 10000 pg/ml
    • 添加回収試験

      89 %

      特定サンプルでの回収試験
      サンプルの種類 平均 % 測定範囲
      Cell culture supernatant 108.3 95% - 117%
      Serum 93.47 85% - 102%
      Plasma 85.73 67% - 99%
    • ステップ

      Multiple steps standard assay
    • 種交差性

      交差種: Pig
    • 製品の概要

      Abcam's IL-6 (Interleukin-6) Pig ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of porcine IL-6 in serum, plasma and cell culture supernatants. 


      This assay employs an antibody specific for pig IL-6 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-6 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-pig IL-6 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-6 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

    • 試験プラットフォーム

      Microplate

    製品の特性

    • 保存方法

      Store at -20°C. Please refer to protocols.
    • 内容 1 x 96 tests
      20X Wash Buffer 1 x 25ml
      320X HRP-Streptavidin Concentrate 1 x 200µl
      5X Assay Diluent 1 x 15ml
      Biotinylated anti-Pig IL-6 2 vials
      IL-6 Microplate (12 x 8 wells) 1 unit
      Recombinant Pig IL-6 Standard (lyophilized) 2 vials
      Stop Solution 1 x 8ml
      TMB One-Step Substrate Reagent 1 x 12ml
    • 研究分野

      • Immunology
      • Innate Immunity
      • Macrophage / Inflamm.
      • Immunology
      • Innate Immunity
      • Cytokines
      • Interleukins
      • Neuroscience
      • Neurology process
      • Metabolism
      • Microbiology
      • Organism
      • Virus
      • RNA Virus
      • ssRNA positive strand virus
      • SARS Coronavirus
      • Cancer
      • Invasion/microenvironment
      • Angiogenesis
      • Angiogenic growth factors
      • Cancer
      • Tumor immunology
      • Cytokines
      • Interleukins
      • Kits/ Lysates/ Other
      • Kits
      • ELISA Kits
      • ELISA Kits
      • Cytokines and cytokine receptors ELISA kits
      • Metabolism
      • Types of disease
      • Obesity
      • Metabolism
      • Types of disease
      • Metabolic disorders
      • Neuroscience
      • Processes
    • 機能

      Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.
    • 関連疾患

      Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
      Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men.
    • 配列類似性

      Belongs to the IL-6 superfamily.
    • 翻訳後修飾

      N- and O-glycosylated.
    • 細胞内局在

      Secreted.
    • Target information above from: UniProt accession P05231 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 別名

      • Interleukin BSF 2
      • B cell differentiation factor
      • B cell stimulatory factor 2
      • B-cell stimulatory factor 2
      • BSF 2
      • BSF-2
      • BSF2
      • CDF
      • CTL differentiation factor
      • Cytotoxic T cell differentiation factor
      • Hepatocyte stimulating factor
      • Hepatocyte stimulatory factor
      • HGF
      • HSF
      • Hybridoma growth factor
      • Hybridoma growth factor Interferon beta-2
      • Hybridoma plasmacytoma growth factor
      • IFN-beta-2
      • IFNB2
      • IL 6
      • IL-6
      • IL6
      • IL6_HUMAN
      • Interferon beta 2
      • Interferon beta-2
      • Interleukin 6
      • Interleukin 6 (interferon beta 2)
      • Interleukin BSF 2
      • Interleukin-6
      see all

    画像

    • Representative Standard Curve
      Representative Standard Curve

      Representative Standard Curve using ab100755

    プロトコール

    • Protocol Booklet

    Click here to view the general protocols

    データシートおよび資料

    • SDS download

    • Datasheet download

      Download

    参考文献 (14)

    ab100755 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab100755 は 14 報の論文で使用されています。

    • Wan K  et al. Effects of dietary calcium pyruvate on gastrointestinal tract development, intestinal health and growth performance of newly weaned piglets fed low-protein diets. J Appl Microbiol 128:355-365 (2020). PubMed: 31618501
    • Zhang E  et al. Comparison of oral and nasal immunization with inactivated porcine epidemic diarrhea virus on intestinal immunity in piglets. Exp Ther Med 20:1596-1606 (2020). PubMed: 32742391
    • Nielsen TK  et al. A Porcine Model for Urinary Tract Infection. Front Microbiol 10:2564 (2019). PubMed: 31824442
    • Huang HN  et al. Epinecidin-1 Protects against Methicillin Resistant Staphylococcus aureus Infection and Sepsis in Pyemia Pigs. Mar Drugs 17:N/A (2019). PubMed: 31835381
    • Zhang ZB  et al. Normothermic Machine Perfusion Protects Against Liver Ischemia-Reperfusion Injury During Reduced-Size Liver Transplantation in Pigs. Ann Transplant 24:9-17 (2019). PubMed: 30607000
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-7 of 7 Abreviews or Q&A

    Abreview for IL6 Pig ELISA Kit

    Good Good 4/5 (Ease of Use)
    Abreviews
    Abreviews

    Sample: Pig Tissue sections (Brain)
    Specification: Brain
    Amount of Sample: 500 µg
    Type of Kit Used: ELISA
    Positive control: WE DO NOT HAVE A POSITIVE CONTROL
    Did you use the Abcam Kit protocol?: Yes
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Oct 16 2012

    Question

    Do you have any data on what a typical level of IL6 in pig plasma would be?

    Read More

    Abcam community

    Verified customer

    Asked on Oct 04 2013

    Answer

    As an example, Il-6 in the tested pig plasma is ˜2ng/ml.

    Read More

    Jeremy Kasanov

    Abcam Scientific Support

    Answered on Oct 04 2013

    Question

    Could you tell me if Elisa kit for pig TNF-alpha (ref ab100756), IL-6 (ab100755) IL-8 (ab113352) are indicated for plasma samples collected using citrated as anticoagulant? ¿Could the sensitivity change if i use another anticoagulant (EDTA, heparin)?
    Thank you in advanced. Kind regards!

    Read More

    Abcam community

    Verified customer

    Asked on Sep 26 2013

    Answer

    I can confirm that the requested kits (ab100755, ab100756 and ab113352) are compatible with citrate as anticoagulant. The sensitivity will not change due to different anticoagulants.

    Read More

    Tanya Bagrij

    Abcam Scientific Support

    Answered on Sep 27 2013

    Question

    estamos muy interesados en probar el kit ab100755 para cuantificar la IL-6 en muestras de tejido cerebral porcino. Debido a que este kit no está testado para dicha aplicación, nos interesa participar en el sistema Abreview.

    Si no he entendido mal, cuando nos enviéis el código podemos hacer la compra del kit. Una vez testado en nuestras muestras os lo comunicamos a través del sistema Abreview y nos enviaríais gratuitamente un kit igual (si nos ha funcionado y lo necesitamos para medir todas nuestras muestras) o bien por otro kit similar si no nos ha funcionado (también estamos interesados en medir IL-1 y TNF-alpha).

    A la espera de tu respuesta. Muchas gracias y un saludo,

    Read More

    Abcam community

    Verified customer

    Asked on Jul 10 2012

    Answer

    DISCOUNT CODE: ******
    Expiration date: DD MM YYYY

    I am very pleased to hear you would like to accept our offer and test ab100755 in Pig tissue samples. This code will give you 1 free kit or value off of order before the expiration date. To redeem this offer, please submit an Abreview forPig tissue samplesand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
    For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.


    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways:
    1) Call to place your order and mention the code to our customer service department;
    2) Include the code in your fax order;
    3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

    Read More

    Abcam Scientific Support

    Answered on Jul 10 2012

    Question

    Llamada de teléfono: ¿Puede usarse el kit en homogenados de tejido cerebral porcino?

    Read More

    Abcam community

    Verified customer

    Asked on Jul 06 2012

    Answer

    Gracias por contactarnos.

    Me comentan mis compañeros que aunque este kit nunca se ha testado con tejidos, dada su versatilidad con distintos tipos de muestras, es muy posible que también funcione con homogenado de cerebro.

    En caso de usar el kit en este tipo de muestra, os aconsejo diluir las muestras al menos 5 veces con Assay Diluent (Item E) para minimizar los efectos de los detergentes en el buffer de lisis. Es muy posible que requieran mayor dilución, pero tendrá que determinarse empíricamente durante el experimento.

    Para el lisado original lo ideal sería comenzar por 1 mg/ml de proteína, o si es posible mas, para alcanzar, una vez diluido, 50-500ug /ml. De nuevo, este rango debe ser tomado como punto de partida y tendrá que ser optimizado empíricamente.

    Para preparar el buffer de lisis, quizás os puedan ayudar los siguientes consejos:

    En general un buffer de lisis debe cumplir los siguientes requisitos:

    a) Bajo contenido en sal (700 mM o menos).

    b) No contener azida sódica.

    c) No contener >0.1% SDS.

    d) No contener > 10mM de agentes reductores (BME o dithiothreitol).

    Esto incluiría cualquier buffer usado par a IP, incluido RIPA buffer.

    En Abcam ofrecemos un servicio de evaluación de anticuerpos y demás productos, llamado Abreview, por el cual os ofrecemos un kit gratuito para una futura compra si adquirís el producto y lo testáis en una aplicación, especie o tipo de muestra no testada hasta ahora, y publicáis los resultados en nuestra website, en forma de Abreview, independientemente de que éstos sean positivos o negativos.

    Para participar en el sistema Abreview:

    1. Contesta a este mail indicando tu interés en participar en el sistema Abreview probando el kit ab100755 en muestras de tejido porcino. En ese momento te enviaremos un código de descuento. Dicho código debe emitirse antes de la compra de ab100755.

    2. Realiza la compra del kit (por mail, fax, online o por teléfono).

    3. Prueba el kit ab100755 en muestras de tejido.

    4. A través del sistema Abreview, comparte tus resultados con nosotros, tanto si son positivos, como negativos (este proceso puede durar unos 10 minutos. Valoramos enormemente si pudierais adjuntar imágenes al Abreview!).

    5. Una vez hayáis publicado la reseña, el código de descuento se activa. Simplemente solicita el pedido por teléfono, fax, o a través de la web, sin olvidar mencionar el código de descuento. El descuento puede ser canjeado por cualquier otro kit y es válido durante 4 meses una vez emitido.

    No dudes en ponerte en contacto con nosotros si tienes cualquier otra consulta sobre esta oferta y estaré encantado de poder ayudarte.

    Las condiciones de uso de esta oferta puede usted consultarlas en www.abcam.com/collaborationdiscount.

    Read More

    Abcam Scientific Support

    Answered on Jul 06 2012

    Question

    As I’m not familiar with these items, I tried to find the ab codes through your site.
    For the items I found a relative code I have update the list.
    For those I don’t have a reference code, this is because either no products matches or all available products reacts for humans when we need them for pigs.
    So please check the list again and advice.
    https://www.abcam.com/Procalcitonin-Human-ELISA-Kit-ab100630.html Porcine Procalcitonin, PCT ELISA kit 96 Tests
    https://www.abcam.com/IL6-Pig-ELISA-Kit-1-x-96-Well-Plate-ab100755.html Porcine IL-6 Quantikine ELISA kit 96 Tests
    https://www.abcam.com/TNF-alpha-Porcine-ELISA-Kit-ab100756.html Porcine TNF-alpha/ TNFSF1A Quantikine ELISA kit 96 Tests
    https://www.abcam.com/Cortisol-Human-ELISA-Kit-ab108674.html Porcine Cortisol ELISA kit 96 Tests ? (Is it the same for humans and pigs?)
    Porcine Noradrenalin ELISA kit 96 Tests (No product match)
    Porcine Adrenaline ELISA kit 96 Tests (No product match)
    https://www.abcam.com/Albumin-Swine-ELISA-Kit-ab108794.html Porcine Serum Cystatin C ELISA Kit 96 Tests
    Porcine Urinary Cystatin C ELISA Kit 96 Tests (Only for humans)
    https://www.abcam.com/IL8-Pig-ELISA-Kit-ab113352.html Porcine Serum NGAL (Neutrophil Gelatinase-Associated Lipocalin) ELISA Kit 96 Tests
    Porcine Urinary NGAL (Neutrophil Gelatinase-Associated Lipocalin) ELISA Kit 96 Tests (Only for humans)
    Porcine Urinary KIM-1 (Kidney Injury Molecule -1) ELISA Kit 96 Tests (Only for humans)
    https://www.abcam.com/IL8-Pig-ELISA-Kit-ab113352.html Porcine Urinary IL-18 (Interleukin -18) ELISA Kit 96 Tests
    Porcine Urinary NAG (N-Acetyl-β-D-Glucosaminidase) ELISA Kit96 Tests (Only for humans)
    https://www.abcam.com/IFN-gamma-Pig-ELISA-Kit-ab113353.html Porcine Urinary L-FABP (Liver-type Fatty-Acid Binding Protein) ELISA Kit 96 Tests

    Read More

    Abcam community

    Verified customer

    Asked on Jun 26 2012

    Answer

    I have received information regarding

    ab119589 Cystatin C Human ELISA Kit
    ab119590 Cystatin C Mouse ELISA Kit
    ab119596 KIM1 Mouse ELISA Kit
    ab119597 KIM1 Rat ELISA Kit
    ab119601 Lipocalin-2 / NGAL Mouse ELISA Kit
    ab119601 Lipocalin-2 / NGAL Mouse ELISA Kit

    these has not been tested with Swine or pig samples so we are unable to confirm the species cross reactivity. It mean only ab100755, ab100756, ab108674, ab108794, ab113352, ab113353 are OK to use with Porcine samples and are guaranteed. Would you like to have a quote for these antibodies.

    I hope this info will be helpful. Should you require more information please do not hesitate to contact me.

    Read More

    Abcam Scientific Support

    Answered on Jun 26 2012

    Question

    As I’m not familiar with these items, I tried to find the ab codes through your site.
    For the items I found a relative code I have update the list.
    For those I don’t have a reference code, this is because either no products matches or all available products reacts for humans when we need them for pigs.
    So please check the list again and advice.
    https://www.abcam.com/Procalcitonin-Human-ELISA-Kit-ab100630.html Porcine Procalcitonin, PCT ELISA kit 96 Tests
    https://www.abcam.com/IL6-Pig-ELISA-Kit-1-x-96-Well-Plate-ab100755.html Porcine IL-6 Quantikine ELISA kit 96 Tests
    https://www.abcam.com/TNF-alpha-Porcine-ELISA-Kit-ab100756.html Porcine TNF-alpha/ TNFSF1A Quantikine ELISA kit 96 Tests
    https://www.abcam.com/Cortisol-Human-ELISA-Kit-ab108674.html Porcine Cortisol ELISA kit 96 Tests ? (Is it the same for humans and pigs?)
    Porcine Noradrenalin ELISA kit 96 Tests (No product match)
    Porcine Adrenaline ELISA kit 96 Tests (No product match)
    https://www.abcam.com/Albumin-Swine-ELISA-Kit-ab108794.html Porcine Serum Cystatin C ELISA Kit 96 Tests
    Porcine Urinary Cystatin C ELISA Kit 96 Tests (Only for humans)
    https://www.abcam.com/IL8-Pig-ELISA-Kit-ab113352.html Porcine Serum NGAL (Neutrophil Gelatinase-Associated Lipocalin) ELISA Kit 96 Tests
    Porcine Urinary NGAL (Neutrophil Gelatinase-Associated Lipocalin) ELISA Kit 96 Tests (Only for humans)
    Porcine Urinary KIM-1 (Kidney Injury Molecule -1) ELISA Kit 96 Tests (Only for humans)
    https://www.abcam.com/IL8-Pig-ELISA-Kit-ab113352.html Porcine Urinary IL-18 (Interleukin -18) ELISA Kit 96 Tests
    Porcine Urinary NAG (N-Acetyl-β-D-Glucosaminidase) ELISA Kit96 Tests (Only for humans)
    https://www.abcam.com/IFN-gamma-Pig-ELISA-Kit-ab113353.html Porcine Urinary L-FABP (Liver-type Fatty-Acid Binding Protein) ELISA Kit 96 Tests

    Read More

    Abcam community

    Verified customer

    Asked on Jun 25 2012

    Answer

    Thank you for contacting us.


    To let you know, we are currently investigating the Porcine (pig) species, cross-reactivity information for following kits ab119602, ab113326, ab119600, ab119601, ab119590 and ab119589. We will send you the details soon.

    The other kits ab100755, ab100756, ab108674, ab108794, ab113352, ab113353 are OK to use with Porcine samples and are guaranteed.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on Jun 25 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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