Pig IL-1 beta ELISA Kit (ab100754)
Key features and details
- Sensitivity: 6 pg/ml
- Range: 8.23 pg/ml - 6000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Pig
製品の概要
-
製品名
Pig IL-1 beta ELISA Kit
IL-1 beta キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 6 pg/ml -
検出範囲
8.23 pg/ml - 6000 pg/ml -
添加回収試験
90 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 96.91 89% - 104% Serum 97.32 88% - 105% Plasma 87.75 80% - 92% -
ステップ
Multiple steps standard assay -
種交差性
交差種: Pig -
製品の概要
Abcam's IL1 beta Pig ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of pig IL1 beta in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for IL1 beta coated on a 96-well plate. Standards and samples are pipetted into the wells and IL1 beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-pig IL1 beta antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL1 beta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
-
試験プラットフォーム
Microplate
製品の特性
-
保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml 600X HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent C 1 x 30ml Biotinylated anti-Pig IL-1 beta 2 vials IL1 beta Microplate (12 x 8 wells) 1 unit Recombinant Pig IL-1 beta Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究分野
-
機能
Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. -
組織特異性
Expressed in activated monocytes/macrophages (at protein level). -
配列類似性
Belongs to the IL-1 family. -
翻訳後修飾
Activation of the IL1B precursor involves a CASP1-catalyzed proteolytic cleavage. Processing and secretion are temporarily associated. -
細胞内局在
Cytoplasm, cytosol. Lysosome. Secreted, exosome. Cytoplasmic vesicle, autophagosome. Secreted. The precursor is cytosolic. In response to inflammasome-activating signals, such as ATP for NLRP3 inflammasome or bacterial flagellin for NLRC4 inflammasome, cleaved and secreted. IL1B lacks any known signal sequence and the pathway(s) of its secretion is(are) not yet fully understood (PubMed:24201029). On the basis of experimental results, several unconventional secretion mechanisms have been proposed. 1. Secretion via secretory lysosomes: a fraction of CASP1 and IL1B precursor may be incorporated, by a yet undefined mechanism, into secretory lysosomes that undergo Ca(2+)-dependent exocytosis with release of mature IL1B (PubMed:15192144). 2. Secretory autophagy: IL1B-containing autophagosomes may fuse with endosomes or multivesicular bodies (MVBs) and then merge with the plasma membrane releasing soluble IL1B or IL1B-containing exosomes (PubMed:24201029). However, autophagy impacts IL1B production at several levels and its role in secretion is still controversial. 3. Secretion via exosomes: ATP-activation of P2RX7 leads to the formation of MVBs containing exosomes with entrapped IL1B, CASP1 and other inflammasome components. These MVBs undergo exocytosis with the release of exosomes. The release of soluble IL1B occurs after the lysis of exosome membranes (By similarity). 4. Secretion by microvesicle shedding: activation of the ATP receptor P2RX7 may induce an immediate shedding of membrane-derived microvesicles containing IL1B and possibly inflammasome components. The cytokine is then released in the extracellular compartment after microvesicle lysis (PubMed:11728343). 5. Release by translocation through permeabilized plasma membrane. This may occur in cells undergoing pyroptosis due to sustained activation of the inflammasome (By similarity). These mechanisms may not be not mutually exclusive. - Information by UniProt
-
別名
- Catabolin
- H1
- IFN beta inducing factor
see all
関連製品
-
SimpleStep ELISA kits
画像
-
Representative Standard Curve
Representative Standard Curve using ab100754
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (5)
ab100754 は 5 報の論文で使用されています。
- Pfeiffer CA et al. Ablation of conceptus PTGS2 expression does not alter early conceptus development and establishment of pregnancy in the pig†. Biol Reprod 102:475-488 (2020). PubMed: 31616930
- Zhang S et al. Immune response in piglets orally immunized with recombinant Bacillus subtilis expressing the capsid protein of porcine circovirus type 2. Cell Commun Signal 18:23 (2020). PubMed: 32046726
- Meyer AE et al. New perspective on conceptus estrogens in maternal recognition and pregnancy establishment in the pig†. Biol Reprod N/A:N/A (2019). PubMed: 31066888
- Huang HN et al. Epinecidin-1 Protects against Methicillin Resistant Staphylococcus aureus Infection and Sepsis in Pyemia Pigs. Mar Drugs 17:N/A (2019). PubMed: 31835381
- Manzke NE et al. Efficacy of energy supplementation on growth performance and immune response of suckling pigs. J Anim Sci 96:4723-4730 (2018). PubMed: 30476156