Pig CRP ELISA Kit (ab205089)
Key features and details
- Sensitivity: 2.15 ng/ml
- Range: 6.25 ng/ml - 200 ng/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Pig
製品の概要
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製品名
Pig CRP ELISA Kit
C Reactive Protein キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Serum < 10% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Serum < 10% -
サンプルの種類
Serum, Hep Plasma, EDTA Plasma, Cit plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
2.15 ng/ml -
検出範囲
6.25 ng/ml - 200 ng/ml -
添加回収試験
> 85 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Serum > 85 6.25ng/ml - 200ng/ml -
全工程の試験時間
1h 10m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Pig -
製品の概要
CRP in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of CRP protein in pig biological samples.
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特記事項
In this assay the CRP present in samples reacts with the anti-CRP antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-CRP antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound CRP. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of CRP in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of CRP in the test sample. The quantity of CRP in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
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試験プラットフォーム
Pre-coated microplate (12 x 8 well strips)
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 100X HRP Conjugated Enzyme Antibody 1 x 150µl 1X Diluent Concentration 1 x 60ml 20X Wash Buffer Concentrate 1 x 50ml Anti-Pig CRP ELISA Microplate 1 unit Chromogen Substrate Solution 1 x 12ml Pig CRP Calibrator (Lyophilized) 1 vial Stop Solution 1 x 12ml -
研究分野
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機能
Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. -
組織特異性
Found in plasma. -
配列類似性
Belongs to the pentaxin family.
Contains 1 pentaxin domain. -
細胞内局在
Secreted. - Information by UniProt
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別名
- Pentraxin 1, short
- C reactive protein
- C reactive protein pentraxin related
see all
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab205089 は 2 報の論文で使用されています。
- Stærk K et al. A Novel Device-Integrated Drug Delivery System for Local Inhibition of Urinary Tract Infection. Front Microbiol 12:685698 (2021). PubMed: 34248906
- Nielsen TK et al. A Porcine Model for Urinary Tract Infection. Front Microbiol 10:2564 (2019). PubMed: 31824442