Anti-PICK1 抗体 [L20/8] (ab252543)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [L20/8] to PICK1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-PICK1 antibody [L20/8]
PICK1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [L20/8] to PICK1 -
由来種
Mouse -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt,ICC/IF,IHC-P or IP -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant fragment. This information is considered to be commercially sensitive.
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ポジティブ・コントロール
- WB: HeLa whole cell lysate. Mouse and rat brain tissue lysate.
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
L20/8 -
アイソタイプ
IgG2a -
研究分野
関連製品
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Alternative Versions
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab252543の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 0.509 µg/ml. Predicted molecular weight: 46 kDa.
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特記事項 |
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WB
Use a concentration of 0.509 µg/ml. Predicted molecular weight: 46 kDa. |
ターゲット情報
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機能
Probable adapter protein that bind to and organize the subcellular localization of a variety of membrane proteins containing some PDZ recognition sequence. Involved in the clustering of various receptors, possibly by acting at the receptor internalization level. Plays a role in synaptic plasticity by regulating the trafficking and internalization of AMPA receptors. May be regulated upon PRKCA activation. May regulate heteromeric ACCN3/ACCN2 channel. -
組織特異性
Ubiquitous. -
配列類似性
Contains 1 AH domain.
Contains 1 PDZ (DHR) domain. -
翻訳後修飾
Phosphorylation at Thr-82 appears to inhibit the interaction with AMPA receptors. -
細胞内局在
Cytoplasm > perinuclear region. Cell junction > synapse. Also present at excitatory synapses. - Information by UniProt
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参照データベース
- Entrez Gene: 9463 Human
- Entrez Gene: 18693 Mouse
- Entrez Gene: 84591 Rat
- Omim: 605926 Human
- SwissProt: Q9NRD5 Human
- SwissProt: Q62083 Mouse
- SwissProt: Q9EP80 Rat
- Unigene: 180871 Human
see all -
別名
- dJ1039K5 antibody
- MGC15204 antibody
- OTTHUMP00000028509 antibody
see all
画像
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Anti-PICK1 antibody [L20/8] (ab252543) at 0.509 µg/ml + HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Secondary
Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Predicted band size: 46 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
This blot was developed using a higher sensitivity ECL substrate.
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All lanes : Anti-PICK1 antibody [L20/8] (ab252543) at 0.509 µg/ml
Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Predicted band size: 46 kDa
Exposure time: 15 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab252543 は 1 報の論文で使用されています。
- Zhang X et al. FSC231 alleviates paclitaxel-induced neuralgia by inhibiting the interactions between PICK1 and GluA2 and activates GSK-3β and ERK1/2. Brain Behav 11:e2380 (2021). PubMed: 34582111