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リコンビナントRabMAb

Anti-PICK1 抗体 [EPR4129(2)] (ab109249)

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Western blot - Anti-PICK1 antibody [EPR4129(2)] (ab109249)
  • Immunoprecipitation - Anti-PICK1 antibody [EPR4129(2)] (ab109249)
  • Anti-PICK1 antibody [EPR4129(2)] (ab109249)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4129(2)] to PICK1
  • Suitable for: WB, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-PICK1 antibody [EPR4129(2)]
    PICK1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR4129(2)] to PICK1

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IPmore details
    適用なし: ICC or IHC-P

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Fetal brain, HeLa, LnCaP and SH-SY5Y cell lysates IP: HeLa whole cell lysate

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab109249の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 47 kDa).
IP
1/30.
特記事項
WB
1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 47 kDa).
IP
1/30.
追加情報
Is unsuitable for ICC or IHC-P.

ターゲット情報

  • 機能

    Probable adapter protein that bind to and organize the subcellular localization of a variety of membrane proteins containing some PDZ recognition sequence. Involved in the clustering of various receptors, possibly by acting at the receptor internalization level. Plays a role in synaptic plasticity by regulating the trafficking and internalization of AMPA receptors. May be regulated upon PRKCA activation. May regulate heteromeric ACCN3/ACCN2 channel.

  • 組織特異性

    Ubiquitous.

  • 配列類似性

    Contains 1 AH domain.
    Contains 1 PDZ (DHR) domain.

  • 翻訳後修飾

    Phosphorylation at Thr-82 appears to inhibit the interaction with AMPA receptors.

  • 細胞内局在

    Cytoplasm > perinuclear region. Cell junction > synapse. Also present at excitatory synapses.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • dJ1039K5 antibody
    • MGC15204 antibody
    • OTTHUMP00000028509 antibody
    • PICK 1 antibody
    • Pick1 antibody
    • PICK1_HUMAN antibody
    • PRKCA binding protein antibody
    • PRKCA-binding protein antibody
    • PRKCABP antibody
    • Protein interacting with C kinase 1 antibody
    • Protein interacting with PRKCA antibody
    • Protein interacting with PRKCA 1 antibody
    • Protein kinase C alpha binding protein antibody
    • Protein kinase C-alpha-binding protein antibody
    • Protein that interacts with C kinase 1 antibody
    see all

画像

  • Western blot - Anti-PICK1 antibody [EPR4129(2)] (ab109249)
    Western blot - Anti-PICK1 antibody [EPR4129(2)] (ab109249)
    All lanes : Anti-PICK1 antibody [EPR4129(2)] (ab109249) at 1/1000 dilution

    Lane 1 : Fetal brain tissue lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : LnCaP cell lysate
    Lane 4 : SH SY5Y cell lysate

    Predicted band size: 47 kDa
    Observed band size: 50 kDa why is the actual band size different from the predicted?

  • Immunoprecipitation - Anti-PICK1 antibody [EPR4129(2)] (ab109249)
    Immunoprecipitation - Anti-PICK1 antibody [EPR4129(2)] (ab109249)

    ab109249 (purified) at 1/30 dilution (20 µg/ml) immunoprecipitating PICK1 in HeLa whole cell lysate.

    Lane 1 (input): HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
    Lane 2 (+): ab109249 & HeLa whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109249 in HeLa whole cell lysate

    For western blotting, ab227648 (purified at 1/500 dilution (0.012 µg/ml) and veriBlot for IP secondary antibody (HRP) (ab131366) at 1/1000 dilution were used.
    Blocking and diluting buffer: 5% NFDM /TBST .

  • Anti-PICK1 antibody [EPR4129(2)] (ab109249)
    Anti-PICK1 antibody [EPR4129(2)] (ab109249)

参考文献 (0)

ab109249 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab109249 は論文での使用が確認できていません。

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