Anti-PICALM 抗体 [EPR12177] (ab172962)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12177] to PICALM
- Suitable for: WB, IHC-P, ICC/IF
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-PICALM antibody [EPR12177]
PICALM 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR12177] to PICALM -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details
適用なし: Flow Cyt or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse -
免疫原
Synthetic peptide within Human PICALM aa 550-650. The exact sequence is proprietary.
Database link: Q13492 -
ポジティブ・コントロール
- WB: U-2 OS, PC-3, Caco-2, HepG2, HeLa, and A431 cell lysates and human fetal brain tissue lysates. IHC-P: Human placenta and breast tissues. ICC/IF: HeLa cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR12177 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab172962の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (4) |
1/1000 - 1/5000. Detects a band of approximately 66-71 kDa (predicted molecular weight: 71 kDa).
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/250.
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特記事項 |
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WB
1/1000 - 1/5000. Detects a band of approximately 66-71 kDa (predicted molecular weight: 71 kDa). |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/250. |
ターゲット情報
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機能
Assembly protein recruiting clathrin and adaptor protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. May be required to determine the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. Involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction. -
組織特異性
Expressed in all tissues examined. -
関連疾患
Note=A chromosomal aberration involving PICALM is found in diffuse histiocytic lymphomas. Translocation t(10;11)(p13;q14) with MLLT10. -
配列類似性
Contains 1 ENTH (epsin N-terminal homology) domain. -
細胞内局在
Membrane > clathrin-coated pit. Golgi apparatus. Cytoplasmic vesicle > clathrin-coated vesicle. Colocalized with clathrin in the Golgi area. - Information by UniProt
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参照データベース
- Entrez Gene: 8301 Human
- Entrez Gene: 233489 Mouse
- Omim: 603025 Human
- SwissProt: Q13492 Human
- SwissProt: Q7M6Y3 Mouse
- Unigene: 163893 Human
- Unigene: 235175 Mouse
- Unigene: 393563 Mouse
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別名
- CALM antibody
- Clathrin assembly lymphoid myeloid leukemia antibody
- Clathrin assembly lymphoid myeloid leukemia protein antibody
see all
画像
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All lanes : Anti-PICALM antibody [EPR12177] (ab172962) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : PICALM knockout HeLa cell lysate
Lane 3 : Caco-2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 71 kDa
Observed band size: 70-75 kDa why is the actual band size different from the predicted?Lanes 1-3: Merged signal (red and green). Green - ab172962 observed at 70-75 kDa. Red - loading control ab8245 observed at 36 kDa.
ab172962 Anti-PICALM antibody [EPR12177] was shown to specifically react with PICALM in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265653 (knockout cell lysate ab258113) was used. Wild-type and PICALM knockout samples were subjected to SDS-PAGE. ab172962 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Immunofluorescent analysis of HeLa cells labeling PICALM with ab172962 at 1/100 dilution.
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All lanes : Anti-PICALM antibody [EPR12177] (ab172962) at 1/5000 dilution
Lane 1 : Wild-type U-2 OS whole cell lysate
Lane 2 : PICALM knockout U-2 OS whole cell lysate
Lane 3 : PC3 (Human prostate adenocarcinoma cell line) whole cell lysate
Lane 4 : Caco-2 (Human colorectal adenocarcinoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 71 kDa
Observed band size: 70-75 kDa why is the actual band size different from the predicted?Lanes 1 - 4: Merged signal (red and green). Green - ab172962 observed at 70-75 kDa. Red - loading control, ab7291 (Mouse anti-Alpha Tubulin [DM1A] observed at 55kDa.
ab172962 was shown to react with PICALM in U-2 OS wild-type cells in Western blot. Loss of signal was observed when PICALM knockout sample was used. U-2 OS wild-type and PICALM knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% Milk in TBS-T (0.1% Tween®) before incubation with ab172962 and ab7291 (Mouse anti-Alpha Tubulin [DM1A] overnight at 4°C at a 1 in 5000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-PICALM antibody [EPR12177] (ab172962) at 1/1000 dilution
Lane 1 : HepG2 lysate
Lane 2 : HeLa lysate
Lane 3 : A431 lysate
Lane 4 : Human fetal brain lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 71 kDa -
Immunohistochemical analysis of paraffin-embedded Human Placenta tissue labeling PICALM with ab172962 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human Breast tissue labeling PICALM with ab172962 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab172962 は 5 報の論文で使用されています。
- Nagamachi A et al. Multiorgan failure with abnormal receptor metabolism in mice mimicking Samd9/9L syndromes. J Clin Invest 131:N/A (2021). PubMed: 33373325
- Willy NM et al. CALM supports clathrin-coated vesicle completion upon membrane tension increase. Proc Natl Acad Sci U S A 118:N/A (2021). PubMed: 34155137
- Wang X et al. DASC, a sensitive classifier for measuring discrete early stages in clathrin-mediated endocytosis. Elife 9:N/A (2020). PubMed: 32352376
- Giovani PA et al. Membrane proteome characterization of periodontal ligament cell sets from deciduous and permanent teeth. J Periodontol 90:775-787 (2019). PubMed: 30499115
- Ramachandran S et al. Cis-acting single nucleotide polymorphisms alter MicroRNA-mediated regulation of human brain-expressed transcripts. Hum Mol Genet 25:4939-4950 (2016). PubMed: 28171541