Anti-PIAS2 抗体 [EPR2582(2)] (ab126601)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2582(2)] to PIAS2
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-PIAS2 antibody [EPR2582(2)]
PIAS2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR2582(2)] to PIAS2 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt,IHC-P or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide within Human PIAS2 aa 100-200. The exact sequence is proprietary.
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ポジティブ・コントロール
- HepG2 whole cell lysate (ab7900); Human fetal kidney tissue lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR2582(2) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab126601の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 63 kDa (predicted molecular weight: 68 kDa).
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特記事項 |
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WB
1/1000 - 1/10000. Detects a band of approximately 63 kDa (predicted molecular weight: 68 kDa). |
ターゲット情報
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機能
Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulator in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing may vary depending upon the biological context and the PIAS2 isoform studied. However, it seems to be mostly involved in gene silencing. Binds to sumoylated ELK1 and enhances its transcriptional activity by preventing recruitment of HDAC2 by ELK1, thus reversing SUMO-mediated repression of ELK1 transactivation activity. Isoform PIAS2-beta, but not isoform PIAS2-alpha, promotes MDM2 sumoylation. Isoform PIAS2-alpha promotes PARK7 sumoylation. Isoform PIAS2-beta promotes NCOA2 sumoylation more efficiently than isoform PIAS2-alpha. Isoform PIAS2-alpha sumoylates PML at'Lys-65' and 'Lys-160'. -
組織特異性
Mainly expressed in testis. Isoform 3 is expressed predominantly in adult testis, weakly in pancreas, embryonic testis and sperm, and at very low levels in other organs. -
パスウェイ
Protein modification; protein sumoylation. -
配列類似性
Belongs to the PIAS family.
Contains 1 PINIT domain.
Contains 1 SAP domain.
Contains 1 SP-RING-type zinc finger. -
発生段階
Isoform 3 expression in adult testis is 14.2-fold stronger than in embryonic testis. -
ドメイン
The LXXLL motif is a transcriptional coregulator signature. -
翻訳後修飾
Sumoylated. -
細胞内局在
Nucleus speckle. Nucleus, PML body. Nucleus. Colocalizes at least partially with promyelocytic leukemia nuclear bodies (PML NBs) (PubMed:22406621). Colocalizes with SUMO1 in nuclear granules (By similarity). - Information by UniProt
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参照データベース
- Entrez Gene: 9063 Human
- Entrez Gene: 17344 Mouse
- Entrez Gene: 83422 Rat
- Omim: 603567 Human
- SwissProt: O75928 Human
- SwissProt: Q8C5D8 Mouse
- SwissProt: Q6AZ28 Rat
- Unigene: 657844 Human
see all -
別名
- Androgen receptor interacting protein 3 antibody
- Androgen receptor-interacting protein 3 antibody
- ARIP3 antibody
see all
画像
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab126601 は 2 報の論文で使用されています。
- Li C et al. Quantitative SUMO proteomics identifies PIAS1 substrates involved in cell migration and motility. Nat Commun 11:834 (2020). PubMed: 32047143
- Sha Z et al. Inhibiting ubiquitination causes an accumulation of SUMOylated newly synthesized nuclear proteins at PML bodies. J Biol Chem 294:15218-15234 (2019). PubMed: 31285264