Name: Anti-PIAS1 antibody [EPR2580(2)]
Brand: RabMAb
SKU: ab109388
Rating: 5 (3 reviews)

Product price, shipping and contact information

Currently unavailable

申し訳ございませんが、現在表示できません。

こちらにお問い合わせいただくか、またはしばらく経ってから改めてお試しください

Loading size & price…

Abpromise

保証された製品品質、優れたカスタマー・サポート。詳細はこちら。

Western blot - Anti-PIAS1 antibody [EPR2580(2)] (ab109388)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR2580(2)] to PIAS1
Suitable for: Flow Cyt, ICC/IF, WB, IHC-P
Reacts with: Mouse, Rat, Human

Alexa Fluor® 594 Alexa Fluor® 647 APC Carrier Free HRP PE

製品名

Anti-PIAS1 antibody [EPR2580(2)]
PIAS1 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [EPR2580(2)] to PIAS1
由来種

Rabbit
アプリケーション

適用あり: Flow Cyt, ICC/IF, WB, IHC-Pmore details
適用なし: IP
種交差性

交差種: Mouse, Rat, Human
免疫原

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
ポジティブ･コントロール
- Human colon tissue. HepG2, Jurkat, 293T, Daudi cell lysates. ICC/IF: HeLa cells
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
解離定数（K_D 値）

K_D = 2.10 x 10 ^-11 M
Learn more about K_D
バッファー

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

EPR2580(2)
アイソタイプ

IgG
研究分野

Alternative Versions
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- Hep G2 nuclear extract lysate (ab14660)

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab109388の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
Flow Cyt		Use at an assay dependent concentration. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF	(1)	1/500.
WB	(2)	1/1000 - 1/10000. Predicted molecular weight: 72 kDa.
IHC-P		1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.

特記事項
Flow Cyt Use at an assay dependent concentration. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/500.
WB 1/1000 - 1/10000. Predicted molecular weight: 72 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.

追加情報

Is unsuitable for IP.

機能

Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. In vitro, binds A/T-rich DNA. The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context. Together with PRMT1, may repress STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling.
組織特異性

Expressed in numerous tissues with highest level in testis.
パスウェイ

Protein modification; protein sumoylation.
配列類似性

Belongs to the PIAS family.
Contains 1 PINIT domain.
Contains 1 SAP domain.
Contains 1 SP-RING-type zinc finger.
ドメイン

The LXXLL motif is a transcriptional coregulator signature.
The SP-RING-type domain is required for promoting EKLF sumoylation.
翻訳後修飾

Sumoylated.
Dimethylated by PRMT1 at Arg-303 in the late phase of interferon gamma (IFN-gamma) signaling, leading to preferential interaction with STAT1 and thus resulting in release of STAT1 from its target gene.
細胞内局在

Nucleus speckle. Interaction with CSRP2 may induce a partial redistribution along the cytoskeleton.
Target information above from: UniProt accession O75925 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 8554 Human
- Entrez Gene: 56469 Mouse
- Entrez Gene: 300772 Rat
- Omim: 603566 Human
- SwissProt: O75925 Human
- SwissProt: O88907 Mouse
- Unigene: 162458 Human
- Unigene: 431253 Mouse
- Unigene: 479179 Mouse
see all
別名
- AR interacting protein antibody
- DDXBP1 antibody
- DEAD/H (Asp-Glu-Ala-Asp/His) box binding protein 1 antibody
- DEAD/H box binding protein 1 antibody
- DEAD/H box-binding protein 1 antibody
- E3 SUMO-protein ligase PIAS1 antibody
- GBP antibody
- Gu binding protein antibody
- Gu-binding protein antibody
- GU/RH-II antibody
- Pias1 antibody
- PIAS1_HUMAN antibody
- Protein inhibitor of activated STAT protein 1 antibody
- Protein inhibitor of activated STAT, 1 antibody
- RNA helicase II binding protein antibody
- RNA helicase II-binding protein antibody
- Zinc finger, MIZ-type containing 3 antibody
- ZMIZ3 antibody
see all

Western blot - Anti-PIAS1 antibody [EPR2580(2)] (ab109388)

Lane 1 : Anti-PIAS1 antibody [EPR2580(2)] (ab109388) at 1/500 dilution
Lane 2 : Anti-GST antibody [EPR4236] (ab111947) at 1/2000 dilution

All lanes : Recombinant Human PIAS1 protein (ab152888)

Lysates/proteins at 0.01 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 72 kDa
Observed band size: 98 kDa why is the actual band size different from the predicted?

Exposure time:
Lane 1: 10 seconds.
Lane 2: 1 minute.

Blocking and dilution buffer: 5% NFDM /TBST.
Immunocytochemistry/ Immunofluorescence - Anti-PIAS1 antibody [EPR2580(2)] (ab109388)

Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling PIAS1 with purified ab109388 at 1/500 dilution (4.6 µg/mL). Cells were fixed in 100% Methanol. Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain.
Western blot - Anti-PIAS1 antibody [EPR2580(2)] (ab109388)

All lanes : Anti-PIAS1 antibody [EPR2580(2)] (ab109388) at 1/1000 dilution

Lane 1 : HepG2 cell lysates
Lane 2 : Jurkat cell lysates
Lane 3 : HEK-293 cell lysates
Lane 4 : Daudi cell lysates

Lysates/proteins at 10 µg per lane.

Predicted band size: 72 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIAS1 antibody [EPR2580(2)] (ab109388)

Immunohistochemical analysis of paraffin-embedded Human colon tissue using 1/100 ab109388.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
OI-RD Scanning - Anti-PIAS1 antibody [EPR2580(2)] (ab109388)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D
Anti-PIAS1 antibody [EPR2580(2)] (ab109388)

Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

Datasheet download

Download

ab109388 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab109388 は 9 報の論文で使用されています。

Zhang K et al. Protein inhibitor of activated STAT1 (PIAS1) inhibits IRF8 activation of Epstein-Barr virus lytic gene expression. Virology 540:75-87 (2020). PubMed: 31743858
Chanda A et al. Identification of the SUMO E3 ligase PIAS1 as a potential survival biomarker in breast cancer. PLoS One 12:e0177639 (2017). WB, IF ; Human . PubMed: 28493978
Scortegagna M et al. The E3 ubiquitin ligase Siah1 regulates adrenal gland organization and aldosterone secretion. JCI Insight 2:N/A (2017). IHC . PubMed: 29212953
Bogachek MV et al. Inhibiting the SUMO Pathway Represses the Cancer Stem Cell Population in Breast and Colorectal Carcinomas. Stem Cell Reports 7:1140-1151 (2016). PubMed: 27916539
Du L et al. Role of SUMO activating enzyme in cancer stem cell maintenance and self-renewal. Nat Commun 7:12326 (2016). IHC-P ; Human . PubMed: 27465491
Campia I et al. An Autocrine Cytokine/JAK/STAT-Signaling Induces Kynurenine Synthesis in Multidrug Resistant Human Cancer Cells. PLoS One 10:e0126159 (2015). WB . PubMed: 25955018
Toropainen S et al. SUMO ligase PIAS1 functions as a target gene selective androgen receptor coregulator on prostate cancer cell chromatin. Nucleic Acids Res 43:848-61 (2015). PubMed: 25552417
Chiou HY et al. Hes-1 SUMOylation by protein inhibitor of activated STAT1 enhances the suppressing effect of Hes-1 on GADD45a expression to increase cell survival. J Biomed Sci 21:53 (2014). WB . PubMed: 24894488
Li R et al. Akt SUMOylation regulates cell proliferation and tumorigenesis. Cancer Res 73:5742-53 (2013). WB ; Human . PubMed: 23884910

レビューと Q&A

Show All レビュー Q&A

レビューを送る質問を送る

1-3 of 3 Abreviews or Q&A

Application

Western blot

Sample

Mouse Cell lysate - nuclear (MEF)

Gel Running Conditions

Reduced Denaturing (8)

Loading amount

20 µg

Specification

MEF

Blocking step

Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Mar 07 2019

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (HeLa)

Permeabilization

Yes - 0.5% Triton 5 minutes

Specification

HeLa

Blocking step

Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Oct 26 2017

Application

Western blot

Sample

Human Cell lysate - whole cell (HeLa)

Gel Running Conditions

Reduced Denaturing

Loading amount

20 µg

Specification

HeLa

Blocking step

Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Mar 31 2017

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Switch on our new BETA site

Now available

In the coming months

Key features and details

Related conjugates and formulations

製品の概要

製品名

製品の詳細

由来種

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

解離定数（KD 値）

バッファー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Alternative Versions

Isotype control

Positive Controls

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

組織特異性

パスウェイ

配列類似性

ドメイン

翻訳後修飾

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

Datasheet download

参考文献 (9)

レビューと Q&A

Western blot abreview for Anti-PIAS1 antibody [EPR2580(2)]

Immunocytochemistry/ Immunofluorescence abreview for Anti-PIAS1 antibody [EPR2580(2)]

Western blot abreview for Anti-PIAS1 antibody [EPR2580(2)]

解離定数（K_D 値）