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RabMAb

Anti-PI3 Kinase p110 beta 抗体 [EPR5515(2)] (ab151549)

製品の概要

  • 製品名
    Anti-PI3 Kinase p110 beta antibody [EPR5515(2)]
    PI3 Kinase p110 beta 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR5515(2)] to PI3 Kinase p110 beta
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide within Human PI3 Kinase p110 beta aa 400-500. The exact sequence is proprietary.

  • ポジティブ・コントロール
    • MCF-7, Jurkat, K562 and 293T cell lysates; Human brain tissue; 293 cells.
  • 特記事項

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab151549 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 123 kDa.

For unpurified use at 1/1000 - 1/10000.

IHC-P 1/75.

See IHC antigen retrieval protocols.

For unpurified use at 1/50 - 1/100.

Flow Cyt 1/20.

For unpurified use at 1/100 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/150.

For unpurified use at 1/250 - 1/500.

ターゲット情報

  • 機能
    Phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2.
  • 組織特異性
    Expressed ubiquitously.
  • パスウェイ
    Phospholipid metabolism; phosphatidylinositol phosphate biosynthesis.
  • 配列類似性
    Belongs to the PI3/PI4-kinase family.
    Contains 1 PI3K/PI4K domain.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 5-bisphosphate 3-kinase 110 kDa catalytic subunit beta antibody
    • 5-bisphosphate 3-kinase catalytic subunit beta isoform antibody
    • DKFZp779K1237 antibody
    • MGC133043 antibody
    • OTTHUMP00000216901 antibody
    • OTTHUMP00000216904 antibody
    • p110 BETA antibody
    • p110Beta antibody
    • Phosphatidylinositol 3 kinase catalytic beta polypeptide antibody
    • Phosphatidylinositol 4 5 bisphosphate 3 kinase 110 kDa catalytic subunit beta antibody
    • Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit beta isoform antibody
    • Phosphatidylinositol-4 antibody
    • Phosphoinositide 3 kinase catalytic beta polypeptide antibody
    • PI3 kinase p110 subunit beta antibody
    • PI3-kinase subunit beta antibody
    • PI3K antibody
    • PI3K beta antibody
    • PI3K-beta antibody
    • PI3Kbeta antibody
    • PI3KCB antibody
    • PIK3C1 antibody
    • Pik3cb antibody
    • PK3CB_HUMAN antibody
    • PtdIns 3 kinase p110 antibody
    • PtdIns-3-kinase subunit beta antibody
    • PtdIns-3-kinase subunit p110-beta antibody
    see all

画像

  • All lanes : Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (ab151549) at 1/1000 dilution

    Lane 1 : Rat Brain Lysate
    Lane 2 : Mouse Brain Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), HRP- conjugated at 1/1000 dilution

    Predicted band size: 123 kDa

  • Overlay histogram showing SH-SY5Y cells stained with ab131260 (red line) at 1/20 dilution. The cells were fixed with 2% Paraformaldehyde. The secondary antibody used was a FITC conjugated goat anti-rabbit IgG at 1/150 dilution. Isotype control antibody (green line) was rabbit monoclonal IgG used under the same conditions.

  • ab151549 staining PI3 Kinase p110 in the HeLa cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody (1/150).ab150082 an Alexa Fluor®555-conjugated Goat anti-rabbit IgG(1/500) was used as the secondary antibody. Nuclei were counterstained with DAPI.

  • ab151549 staining PI3 Kinase p110 beta in Human transitional cell carcinoma of bladder tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/75). An undiluted HRP-conjugated mouse anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.

  • Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (ab151549) at 1/5000 dilution + K562 Cell Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), HRP- conjugated at 1/1000 dilution

    Predicted band size: 123 kDa
    Observed band size: 110 kDa
    why is the actual band size different from the predicted?

  • All lanes : Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (ab151549) at 1/1000 dilution

    Lane 1 : 293 Cell Lysate
    Lane 2 : Jurket Cell Lysate
    Lane 3 : MCF-7 Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), HRP- conjugated at 1/1000 dilution

    Predicted band size: 123 kDa
    Observed band size: 110 kDa why is the actual band size different from the predicted?

  • Overlay histogram showing HeLa cells stained with unpurified ab151549 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab151549, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • All lanes : Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (ab151549) at 1/1000 dilution (unpurified)

    Lane 1 : MCF7 cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : K562 cell lysate
    Lane 4 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 123 kDa

  • Immunohistochemical analysis of paraffin-embedded Human brain tissue labelling PI3 Kinase p110 beta with unpurified ab151549 at 1/50 dilution.

  • Immunofluorescent analysis of 293 cells labelling PI3 Kinase p110 beta with unpurified ab151549 at 1/10 dilution.

  • Immunohistochemical analysis of paraffin embedded Human Cervical carcinoma tissue using unpurified ab151549 showing +ve staining.

  • Immunohistochemical analysis of paraffin embedded normal Human colon tissue using unpurified ab151549 showing +ve staining.

  • Immunohistochemical analysis of paraffin embedded Human Endometrial carcinoma tissue using unpurified ab151549 showing +ve staining.

  • Immunohistochemical analysis of paraffin embedded normal Human kidney tissue using unpurified ab151549 showing +ve staining.

  • Immunohistochemical analysis of paraffin embedded Human Skeletal muscle tissue using unpurified ab151549 showing +ve staining.

参考文献

This product has been referenced in:
  • Yang S  et al. Effect of FGF-21 on implant bone defects through hepatocyte growth factor (HGF)-mediated PI3K/AKT signaling pathway. Biomed Pharmacother 109:1259-1267 (2019). Read more (PubMed: 30551376) »
  • Deng R  et al. lncRNA SNHG1 negatively regulates miRNA-101-3p to enhance the expression of ROCK1 and promote cell proliferation, migration and invasion in osteosarcoma. Int J Mol Med 43:1157-1166 (2019). Read more (PubMed: 30592267) »
See all 28 Publications for this product

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