Anti-PI-9 抗体 (ab233443)
Key features and details
- Rabbit polyclonal to PI-9
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-PI-9 antibody
PI-9 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PI-9 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Human -
免疫原
Recombinant full length protein (His-T7-tag) corresponding to Human PI-9 aa 1 to the C-terminus. (Expressed in E.coli).
Database link: P50453 -
ポジティブ・コントロール
- IHC-P: Human stomach, cerebrum, prostate cancer, kidney, prostate and bile duct cancer tissues. WB: Recombinant human PI-9 protein; Human serum; Mouse placenta lysate; K562 cell lysate. ICC/IF: HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab233443 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab233443の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 42 kDa.
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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ICC/IF |
Use a concentration of 5 - 20 µg/ml.
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特記事項 |
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WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 42 kDa. |
IHC-P
Use a concentration of 5 - 20 µg/ml. |
ICC/IF
Use a concentration of 5 - 20 µg/ml. |
ターゲット情報
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機能
Granzyme B inhibitor. -
配列類似性
Belongs to the serpin family. Ov-serpin subfamily. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 5272 Human
- Entrez Gene: 20723 Mouse
- Omim: 601799 Human
- SwissProt: P50453 Human
- Unigene: 104879 Human
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別名
- CAP 3 antibody
- CAP-3 antibody
- CAP3 antibody
see all
画像
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Formalin-fixed, paraffin-embedded human bile duct cancer tissue stained for PI-9 using ab233443 at 10 µg/ml in immunohistochemical analysis.
Secondary Antibody: 2 µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
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Anti-PI-9 antibody (ab233443) at 2 µg/ml + Mouse placenta lysate
Predicted band size: 42 kDa -
Anti-PI-9 antibody (ab233443) at 2 µg/ml + K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate
Predicted band size: 42 kDa -
Anti-PI-9 antibody (ab233443) at 3 µg/ml + Human serum
Predicted band size: 42 kDa -
Anti-PI-9 antibody (ab233443) at 3 µg/ml + Recombinant human PI-9 protein
Secondary
HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 42 kDa -
HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling PI-9 using ab233443 at 10 µg/ml in ICC/IF. FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody was used as the secondary antibody at 1 µg/ml.
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Formalin-fixed, paraffin-embedded human prostate tissue stained for PI-9 using ab233443 at 10 µg/ml in immunohistochemical analysis.
Secondary Antibody: 2 µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
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Formalin-fixed, paraffin-embedded human kidney tissue stained for PI-9 using ab233443 at 10 µg/ml in immunohistochemical analysis.
Secondary Antibody: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
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Formalin-fixed, paraffin-embedded human prostate cancer tissue stained for PI-9 using ab233443 at 10 µg/ml in immunohistochemical analysis.
Secondary Antibody: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
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Formalin-fixed, paraffin-embedded human cerebrum tissue stained for PI-9 using ab233443 at 10 µg/ml in immunohistochemical analysis.
Secondary Antibody: 2 µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
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Formalin-fixed, paraffin-embedded human stomach tissue stained for PI-9 using ab233443 at 10 µg/ml in immunohistochemical analysis.
Secondary Antibody: 2 µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab233443 は論文での使用が確認できていません。