Anti-PI 3 Kinase p110 delta 抗体 (ab1678)

Reviews (3)Q&A (3)References (15)

製品の概要

  • 製品名

    Anti-PI 3 Kinase p110 delta antibody
    PI 3 Kinase p110 delta 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to PI 3 Kinase p110 delta

  • 由来種

    Rabbit

  • 特異性

    Binds to a 110 Kd protein. Not likely to cross react with other isoforms due to sequence dissimilarity.

  • アプリケーション

    適用あり: ICC/IF, IP, WB, ELISA, ICCmore details

  • 種交差性

    交差種: Mouse, Human

  • 免疫原

    20 amino acid C-terminal peptide to Phosphoinositide 3 Kinase p110 delta

  • ポジティブ･コントロール

    • ICC/IF: HeLa cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab1678 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 µg/ml.
IP Use at an assay dependent concentration. PubMed: 23023391
WB 1/2000 - 1/3000.
ELISA 1/75000.
ICC 1/400.

ターゲット情報

  • 組織特異性

    Expressed predominantly in leukocytes.

  • パスウェイ

    Phospholipid metabolism; phosphatidylinositol phosphate biosynthesis.

  • 配列類似性

    Belongs to the PI3/PI4-kinase family.
    Contains 1 PI3K/PI4K domain.

  • 翻訳後修飾

    Autophosphorylation on Ser-1039 results in the almost complete inactivation of the lipid kinase activity.
  • Information by UniProt

  • 参照データベース

    • 別名

      • 5-bisphosphate 3-kinase 110 kDa catalytic subunit delta antibody
      • 5-bisphosphate 3-kinase catalytic subunit delta isoform antibody
      • APDS antibody
      • GRB1 antibody
      • IMD14 antibody
      • p110d antibody
      • p110delta antibody
      • p110dp85a antibody
      • p85-ALPHA antibody
      • Phosphatidylinositol 3 kinase catalytic delta polypeptide antibody
      • Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit delta isoform antibody
      • Phosphatidylinositol 4,5 bisphosphate 3 kinase 110 kDa catalytic subunit delta antibody
      • Phosphatidylinositol 4,5 bisphosphate 3 kinase, catalytic subunit delta antibody
      • Phosphatidylinositol 45 bisphosphate 3 kinase catalytic subunit delta isoform antibody
      • Phosphatidylinositol-4 antibody
      • Phosphoinositide 3 kinase B antibody
      • Phosphoinositide 3 kinase C antibody
      • Phosphoinositide 3 kinase catalytic delta polypeptide antibody
      • Phosphoinositide 3 kinase, catalytic, delta polypeptide variant p37delta antibody
      • PI3 kinase p110 subunit delta antibody
      • PI3-kinase subunit delta antibody
      • PI3K antibody
      • PI3K-delta antibody
      • PI3Kdelta antibody
      • Pik3cd antibody
      • PIK3R1 antibody
      • PK3CD antibody
      • PK3CD_HUMAN antibody
      • PtdIns 3 kinase p110 antibody
      • PtdIns 3 kinase subunit p110 delta antibody
      • PtdIns-3-kinase subunit delta antibody
      • PtdIns-3-kinase subunit p110-delta antibody
      see all

    画像

    • Western blot - Anti-PI 3 Kinase p110 delta antibody (ab1678)
      Western blot - Anti-PI 3 Kinase p110 delta antibody (ab1678)Abreview from Verifed customer
      All lanes : Anti-PI 3 Kinase p110 delta antibody (ab1678) at 1/2000 dilution

      Lane 1 : Peripheral Blood Mononuclear Cells (PBMCs)
      Lane 2 : Primary human monocytes
      Lane 3 : Primary human macropahges
      Lane 4 : U937 monocytic cell line
      Lane 5 : Whole human Lung

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Bovine anti-Rabbit IgG-HRP at 1/2000 dilution

      Developed using the ECL technique.

      Exposure time: 1 minute

      See Abreview

    • Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase p110 delta antibody (ab1678)
      Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase p110 delta antibody (ab1678)

      ICC/IF image of ab1678 stained HeLa (human epithelial cell line from cervix adenocarcinoma) cells. The cells were 4% formaldehyde fixed (10 minutes) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1678, 1 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1 hour. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1 hour. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 µM.

    参考文献

    This product has been referenced in:

    • Shin YJ  et al. PIP4K2A as a negative regulator of PI3K in PTEN-deficient glioblastoma. J Exp Med 216:1120-1134 (2019). Read more (PubMed: 30898893) »
    • Goulielmaki E  et al. Pharmacological inactivation of the PI3K p110d prevents breast tumour progression by targeting cancer cells and macrophages. Cell Death Dis 9:678 (2018). Read more (PubMed: 29880805) »
    See all 15 Publications for this product

    ab1678 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    レビューと Q&A

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    レビューを送る 質問を送る

    1-6 of 6 Abreviews or Q&A

    Western blot abreview for Anti-PI 3 Kinase p110 delta antibody

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (K652 cells)
    Loading amount
    5000 cells
    Specification
    K652 cells
    Treatment
    DMSO or 1 uM CAL-101 for 24 hours
    Gel Running Conditions
    Reduced Denaturing (4-20% Bis-Tris)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    投稿 Mar 21 2013

    Question

    Hello,

    We have a quality complaint for item ab55593. Please check attached file for detail.

    We request money back.

    Thanks,


    Item number: ab55593

    Order number: 1049169



    General Information

    Antibody storage conditions？





    -20









    Description of the problem (high background, wrong band size, more bands, no band etc.)





    no band









    Sample (Species/Cell extract/Nuclear extract/Purified protein/Recombinant protein etc.)





    Human/Cell extract









    Sample preparation (Buffer/Protease inhibitors/Heating sample etc.)





    RIPA buffer containing protease and phosphatase inhibitor cocktails (Roche Diagnostics) and also heating sample







    Amount of protein loaded





    60, 80μg of total protein extracts









    Electrophoresis/Gel conditions (Reducing or Non-reducing gel, % of the gel etc.)





    10% SDS-PAGE gels









    Transfer and blocking conditions (Buffer/time period, Blocking agent etc.)





    nitrocellulose membranes (Milliopore) 90 min

    The membranes were blocked with 5% milk powder, washed with TBST







    Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)





    Primeary antibody purchased from Abcom named ab55593

    Primary antibody is diluted with 4% BSA. And the membranes were incubated overnight at 4℃. The membranes were washed with TBST three times.







    Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)





    peroxidase-conjugated anti-mouse IgG (Sigma) as a secondary antibody

    Incubation for 2 hours was at room temperature.

    Also washed three times by TBST.







    Detection method (ECL, ECLPlus etc.)





    Western Blotting luminal reagent (Santa cruz)







    Positive and negative controls used (please specify)





    Gapdh as positive control









    Instruction

    Repeat times: 3


    Our western blotting experiment is based on the instruction provided by your company, but we got no band. At the same time, we used the protein of 293T cell (published by some paper) to repeat the experiment and we got the same result.

    We have prolonged the time of the incubation and exposure, and also use the Concentration 1:200, but there is still no band of PI3KCB. There is no problem with our technology of western blotting, because we have got the target band of PI3KC3 by antibody from CST.

    Finally, I hope that your company can make up our loss and solve the problems as soon as possible.

    Read More
    Answer

    Thank you for your enquiry regarding ab55593 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody.

    I would like to reassure you that our Abpromise applies to your complaint since you purchased this product within the guarantee period. This means that in the event that a product is not functioning in the applications/species cited on the product data sheet (and the problem has been reported within 6 months of purchase) we will happily offer a credit note/refund to the value of the product purchased.

    As the datasheet indicates this antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Would you be so kind to confirm the sample types used (species, cells, tissues)?

    We have several alternative antibodies against the same target (PI3 Kinase p110 beta - ab32569, ab97322, ab1678) and I could offer you a free of charge replacement, or a credit note or a full refund as compensation.

    Thank you for your understanding and co-operation in this matter. I look forward to hearing from you and hope to solve this problem as soon as possible.

    Read More

    Question

    I recently purchased your PI3K p110 delta antibody (ab1678). You will be pleased to hear that the antibody appears to work, since recombinant PI3K p110 delta protein appears positive.

    However, if you observe, in the cell sample lanes (3 samples indicated by #number) another band appears around 37kDa.

    Recently a paper was published: p37delta is a new isoform of PI3K p110delta that increases cell proliferation and is over expressed in tumors.
    http://www.nature.com/onc/journal/vaop/ncurrent/full/onc2011492a.html

    It would be really helpful therefore to know exactly the sequence for the immunogen. Your data sheet just says 20 amino acid C-terminal peptide to PI3K p110 delta, which is not enough to know whether it would pick up the p37delta described in the paper above.

    It would be good to know what this other band the antibody picks up is.

    I await hearing your response.

    Read More
    Answer

    Thank you for your data and for bringing this article to our attention.

    The immunogen sequence is

    *************

    Is that included in the p37delta sequence?

    I am glad to see the antibody was specific for the p110 delta isoform. I encourage you to submit your results in an Abreview. There are currently two examples of western blot reviews on the online datasheet.

    https://www.abcam.com/pi-3-kinase-p110-delta-antibody-ab1678-reviews.html

    For information on how to submit a review (for which you will receive credit for a future purchase or gift card), please see the page at this link:

    www.abcam.com/abreviews

    Read More

    Western blot abreview for Anti-PI 3 Kinase p110 delta antibody

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (monocyte, macrophage, whole lung)
    Loading amount
    20 µg
    Specification
    monocyte, macrophage, whole lung
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris gel)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    投稿 Sep 03 2009

    Western blot abreview for Anti-PI 3 Kinase p110 delta antibody

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (cancer cells)
    Loading amount
    15 µg
    Specification
    cancer cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Read More

    Abcam user community

    Verified customer

    投稿 Jun 07 2007

    Question

    Mr kamal puri (kpuri@icos.com) Is PI3Kinase antibody (ab1678) a blocking antibody?

    Read More
    Answer

    We have no evidence that PI3Kinase ab1678 is a blocking antibody

    Read More

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