製品の概要
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製品名Anti-PI 3 Kinase p110 delta antibody
PI 3 Kinase p110 delta 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to PI 3 Kinase p110 delta
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由来種Rabbit
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特異性Binds to a 110 Kd protein. Not likely to cross react with other isoforms due to sequence dissimilarity.
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アプリケーション適用あり: ICC/IF, IP, WB, ELISA, ICCmore details
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種交差性交差種: Mouse, Human
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免疫原
20 amino acid C-terminal peptide to Phosphoinositide 3 Kinase p110 delta
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ポジティブ･コントロール
- ICC/IF: HeLa cells.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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バッファーPreservative: 0.1% Sodium azide
Constituent: 0.1% BSA -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab1678 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | Use a concentration of 1 µg/ml. | |
| IP | Use at an assay dependent concentration. PubMed: 23023391 | |
| WB | 1/2000 - 1/3000. | |
| ELISA | 1/75000. | |
| ICC | 1/400. |
ターゲット情報
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組織特異性Expressed predominantly in leukocytes.
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パスウェイPhospholipid metabolism; phosphatidylinositol phosphate biosynthesis.
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配列類似性Belongs to the PI3/PI4-kinase family.
Contains 1 PI3K/PI4K domain. -
翻訳後修飾Autophosphorylation on Ser-1039 results in the almost complete inactivation of the lipid kinase activity.
- Information by UniProt
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参照データベース
- Entrez Gene: 5293 Human
- Entrez Gene: 18707 Mouse
- Omim: 602839 Human
- SwissProt: O00329 Human
- SwissProt: O35904 Mouse
- Unigene: 518451 Human
- Unigene: 229108 Mouse
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別名
- 5-bisphosphate 3-kinase 110 kDa catalytic subunit delta antibody
- 5-bisphosphate 3-kinase catalytic subunit delta isoform antibody
- APDS antibody
see all
画像
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Western blot - Anti-PI 3 Kinase p110 delta antibody (ab1678)Abreview from Verifed customerAll lanes : Anti-PI 3 Kinase p110 delta antibody (ab1678) at 1/2000 dilution
Lane 1 : Peripheral Blood Mononuclear Cells (PBMCs)
Lane 2 : Primary human monocytes
Lane 3 : Primary human macropahges
Lane 4 : U937 monocytic cell line
Lane 5 : Whole human Lung
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Bovine anti-Rabbit IgG-HRP at 1/2000 dilution
Developed using the ECL technique.
Exposure time: 1 minute
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Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase p110 delta antibody (ab1678)ICC/IF image of ab1678 stained HeLa (human epithelial cell line from cervix adenocarcinoma) cells. The cells were 4% formaldehyde fixed (10 minutes) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1678, 1 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1 hour. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1 hour. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 µM.
プロトコール
参考文献
This product has been referenced in:
- Goulielmaki E et al. Pharmacological inactivation of the PI3K p110d prevents breast tumour progression by targeting cancer cells and macrophages. Cell Death Dis 9:678 (2018). Read more (PubMed: 29880805) »
- Li J et al. Down-regulation of microRNA-27b promotes retinal pigment epithelial cell proliferation and migration by targeting Nox2. Pathol Res Pract 214:925-933 (2018). WB ; Human . Read more (PubMed: 29858119) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"