Anti-PI 3 Kinase p110 delta 抗体 (ab1678)
Key features and details
- Rabbit polyclonal to PI 3 Kinase p110 delta
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-PI 3 Kinase p110 delta antibody
PI 3 Kinase p110 delta 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PI 3 Kinase p110 delta -
由来種
Rabbit -
特異性
Binds to a 110 Kd protein. Not likely to cross react with other isoforms due to sequence dissimilarity. -
アプリケーション
適用あり: ICC/IFmore details -
種交差性
交差種: Human -
免疫原
20 amino acid C-terminal peptide to Phosphoinositide 3 Kinase p110 delta
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特記事項
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.1% Sodium azide
Constituent: 0.1% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab1678 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | Use a concentration of 1 µg/ml. |
ターゲット情報
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組織特異性
Expressed predominantly in leukocytes. -
パスウェイ
Phospholipid metabolism; phosphatidylinositol phosphate biosynthesis. -
配列類似性
Belongs to the PI3/PI4-kinase family.
Contains 1 PI3K/PI4K domain. -
翻訳後修飾
Autophosphorylation on Ser-1039 results in the almost complete inactivation of the lipid kinase activity. - Information by UniProt
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参照データベース
- Entrez Gene: 5293 Human
- Omim: 602839 Human
- SwissProt: O00329 Human
- Unigene: 518451 Human
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別名
- 5-bisphosphate 3-kinase 110 kDa catalytic subunit delta antibody
- 5-bisphosphate 3-kinase catalytic subunit delta isoform antibody
- APDS antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase p110 delta antibody (ab1678)
ICC/IF image of ab1678 stained HeLa (human epithelial cell line from cervix adenocarcinoma) cells. The cells were 4% formaldehyde fixed (10 minutes) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1678, 1 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1 hour. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1 hour. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 µM.
参考文献 (21)
ab1678 は 21 報の論文で使用されています。
- Shin YJ et al. PIP4K2A as a negative regulator of PI3K in PTEN-deficient glioblastoma. J Exp Med 216:1120-1134 (2019). PubMed: 30898893
- Mei XL & Zhong S Long noncoding RNA LINC00520 prevents the progression of cutaneous squamous cell carcinoma through the inactivation of the PI3K/Akt signaling pathway by downregulating EGFR. Chin Med J (Engl) 132:454-465 (2019). PubMed: 30707166
- Feng Z et al. CD40L inhibits cell growth of THP-1 cells by suppressing the PI3K/Akt pathway. Onco Targets Ther 12:3011-3017 (2019). PubMed: 31114244
- Romeo V et al. Effects of PI3K Inhibition on Afucosylated Antibody-Driven Fc?RIIIa Events and Phospho-S6 Activity in NK Cells. J Immunol 203:137-147 (2019). PubMed: 31092639
- Xu D et al. Ligustrazine Inhibits Growth, Migration and Invasion of Medulloblastoma Daoy Cells by Up-Regulation of miR-211. Cell Physiol Biochem 49:2012-2021 (2018). PubMed: 30244244
