Anti-VPS34 抗体 - N-terminal (ab227861)
Key features and details
- Rabbit polyclonal to VPS34 - N-terminal
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-VPS34 antibody - N-terminal
VPS34 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to VPS34 - N-terminal -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow, Dog, Pig, Xenopus laevis -
免疫原
Recombinant fragment within Human VPS34 (N terminal). The exact sequence is proprietary.
Database link: Q8NEB9 -
ポジティブ・コントロール
- WB: HEK-293T, A431, HeLa, HepG2, U-87 MG, SK-N-SH, IMR32 and SK-N-AS whole cell lysates; Wild-type and VPS34 transfected HEK-293T whole cell extracts. IP: HeLa whole cell extract.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab227861の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/10000. Predicted molecular weight: 102 kDa.
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IP |
1/100 - 1/500.
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特記事項 |
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WB
1/500 - 1/10000. Predicted molecular weight: 102 kDa. |
IP
1/100 - 1/500. |
ターゲット情報
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機能
Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate. Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for the abcission step in cytokinesis. -
組織特異性
Ubiquitously expressed, with a highest expression in skeletal muscle. -
配列類似性
Belongs to the PI3/PI4-kinase family.
Contains 1 PI3K/PI4K domain. -
細胞内局在
Midbody. - Information by UniProt
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参照データベース
- Entrez Gene: 534815 Cow
- Entrez Gene: 490477 Dog
- Entrez Gene: 5289 Human
- Entrez Gene: 225326 Mouse
- Entrez Gene: 503700 Pig
- Entrez Gene: 65052 Rat
- Entrez Gene: 446671 Xenopus laevis
- Omim: 602609 Human
see all -
別名
- hVps34 antibody
- MGC61518 antibody
- Phosphatidylinositol 3 kinase catalytic subunit type 3 antibody
see all
画像
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All lanes : Anti-VPS34 antibody - N-terminal (ab227861) at 1/1000 dilution
Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : VPS34 shRNA transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 102 kDa7.5% SDS-PAGE gel.
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All lanes : Anti-VPS34 antibody - N-terminal (ab227861) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 102 kDa7.5% SDS-PAGE gel.
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All lanes : Anti-VPS34 antibody - N-terminal (ab227861) at 1/1000 dilution
Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
Lane 2 : SK-N-SH (human neuroblastoma cell line) whole cell lysate
Lane 3 : IMR32 whole cell lysate
Lane 4 : SK-N-AS whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 102 kDa7.5% SDS-PAGE gel.
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All lanes : Anti-VPS34 antibody - N-terminal (ab227861) at 1/5000 dilution
Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : VPS34 transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 102 kDa5% SDS-PAGE gel.
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VPS34 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract with 5 µg of ab227861. Western blot was performed from the immunoprecipitate using ab227861. Anti-Rabbit IgG was used as a secondary reagent.
Lane 1: HeLa whole cell extract.
Lane 2: Control IgG IP in HeLa whole cell extract.
Lane 3: ab227861 IP in HeLa whole cell extract.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab227861 は 2 報の論文で使用されています。
- Wrobel L et al. Compounds activating VCP D1 ATPase enhance both autophagic and proteasomal neurotoxic protein clearance. Nat Commun 13:4146 (2022). PubMed: 35842429
- Huang W et al. AHCYL1 senses SAH to inhibit autophagy through interaction with PIK3C3 in an MTORC1-independent manner. Autophagy N/A:1-11 (2021). PubMed: 33993848