Anti-VPS34 抗体 (ab233437)
Key features and details
- Rabbit polyclonal to VPS34
- Suitable for: WB, IHC-P
- Reacts with: Human, Pig
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-VPS34 antibody
VPS34 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to VPS34 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human, Pig
交差が予測される動物種: Mouse, Rat -
免疫原
Recombinant fragment corresponding to Human VPS34 aa 600 to the C-terminus. Expressed in E.coli.
Database link: Q8NEB9 -
ポジティブ・コントロール
- WB: Recombinant human VPS34 protein; HeLa cell lysate; Pig brain lysate. IHC-P: Human breast cancer, brain, liver and pancreas tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab233437の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 102 kDa.
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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特記事項 |
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WB
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 102 kDa. |
IHC-P
Use a concentration of 5 - 20 µg/ml. |
ターゲット情報
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機能
Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate. Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for the abcission step in cytokinesis. -
組織特異性
Ubiquitously expressed, with a highest expression in skeletal muscle. -
配列類似性
Belongs to the PI3/PI4-kinase family.
Contains 1 PI3K/PI4K domain. -
細胞内局在
Midbody. - Information by UniProt
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参照データベース
- Entrez Gene: 5289 Human
- Entrez Gene: 225326 Mouse
- Entrez Gene: 503700 Pig
- Entrez Gene: 65052 Rat
- Omim: 602609 Human
- SwissProt: Q8NEB9 Human
- SwissProt: Q6PF93 Mouse
- SwissProt: Q5D891 Pig
see all -
別名
- hVps34 antibody
- MGC61518 antibody
- Phosphatidylinositol 3 kinase catalytic subunit type 3 antibody
see all
画像
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Anti-VPS34 antibody (ab233437) at 3 µg/ml + Recombinant human VPS34 protein
Secondary
HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 102 kDa -
All lanes : Anti-VPS34 antibody (ab233437) at 3 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : Pig brain lysate
Secondary
All lanes : HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 102 kDa -
Formalin-fixed, paraffin-embedded human breast cancer tissue stained for VPS34 using ab233437 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded human cerebrum tissue stained for VPS34 using ab233437 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded human liver tissue stained for VPS34 using ab233437 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded human pancreatic cancer tissue stained for VPS34 using ab233437 at 20 μg/ml in immunohistochemical analysis. DAB staining.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab233437 は論文での使用が確認できていません。