Key features and details
- Mouse monoclonal [4F3] to PI 3 Kinase catalytic subunit alpha/PIK3CA
- Suitable for: WB, ELISA, Flow Cyt, ICC/IF
- Reacts with: Human
- Isotype: IgG1
製品名Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [4F3]
PI 3 Kinase catalytic subunit alpha/PIK3CA 一次抗体 製品一覧
製品の詳細Mouse monoclonal [4F3] to PI 3 Kinase catalytic subunit alpha/PIK3CA
アプリケーション適用あり: WB, ELISA, Flow Cyt, ICC/IFmore details
Recombinant fragment corresponding to Human PI 3 Kinase catalytic subunit alpha/PIK3CA.
- Recombinant Human PI 3 Kinase catalytic subunit alpha/PIK3CA protein (AA: 881-1068), HEK293 cell lysate and PI 3 Kinase catalytic subunit alpha/PIK3CA (AA: 881-1068)-hIgGFc transfected HEK293 cell lysate.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term.
バッファーPreservative: 0.05% Sodium azide
Constituent: 99% PBS
Concentration information loading...
精製度Protein G purified
特記事項（精製）Purified from tissue culture supernatant.
Our Abpromise guarantee covers the use of ab126819 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 47.4, 124 kDa.|
|Flow Cyt||1/200 - 1/400.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/200 - 1/1000.|
機能Phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2.
関連疾患Defects in PIK3CA are associated with colorectal cancer (CRC) [MIM:114500].
Defects in PIK3CA are a cause of susceptibility to breast cancer (BC) [MIM:114480]. A common malignancy originating from breast epithelial tissue. Breast neoplasms can be distinguished by their histologic pattern. Invasive ductal carcinoma is by far the most common type. Breast cancer is etiologically and genetically heterogeneous. Important genetic factors have been indicated by familial occurrence and bilateral involvement. Mutations at more than one locus can be involved in different families or even in the same case.
Defects in PIK3CA are a cause of susceptibility to ovarian cancer (OC) [MIM:167000]. Ovarian cancer common malignancy originating from ovarian tissue. Although many histologic types of ovarian neoplasms have been described, epithelial ovarian carcinoma is the most common form. Ovarian cancers are often asymptomatic and the recognized signs and symptoms, even of late-stage disease, are vague. Consequently, most patients are diagnosed with advanced disease.
Defects in PIK3CA may underlie hepatocellular carcinoma (HCC) [MIM:114550].
Defects in PIK3CA are a cause of keratosis seborrheic (KERSEB) [MIM:182000]. A common benign skin tumor. Seborrheic keratoses usually begin with the appearance of one or more sharply defined, light brown, flat macules. The lesions may be sparse or numerous. As they initially grow, they develop a velvety to finely verrucous surface, followed by an uneven warty surface with multiple plugged follicles and a dull or lackluster appearance.
配列類似性Belongs to the PI3/PI4-kinase family.
Contains 1 C2 domain.
Contains 1 PI3K/PI4K domain.
- Information by UniProt
- 5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha antibody
- 5-bisphosphate 3-kinase catalytic subunit alpha isoform antibody
- caPI3K antibody
Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [4F3] (ab126819) at 1/500 dilution + Recombinant Human PI 3 Kinase catalytic subunit alpha/PIK3CA protein (AA: 881-1068)
Predicted band size: 47.4, 124 kDa
All lanes : Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [4F3] (ab126819) at 1/500 dilution
Lane 1 : HEK293 cell lysate
Lane 2 : PI 3 Kinase catalytic subunit alpha/PIK3CA (AA: 881-1068)-hIgGFc transfected HEK293 cell lysate
Predicted band size: 47.4, 124 kDa
Example of standard curves obtained using ab126819. Control antigen 100ng (black), antigen 10ng (purple), antigen 50ng (blue), antigen 100ng (red).
Immunofluorescence analysis of HeLa cells using ab126819 at 1/200 (green). Blue: DRAQ5 fluorescent DNA dye.
Flow cytometric analysis of Jurkat cells using ab126819 at 1/200 (green) and negative control (red).