Anti-Phosphotyrosine 抗体 [IG2] (ab5592)

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Immunocytochemistry/ Immunofluorescence - Anti-Phosphotyrosine antibody [IG2] (ab5592)

    Key features and details

    • Mouse monoclonal [IG2] to Phosphotyrosine
    • Suitable for: ELISA, ICC/IF, WB, ICC, Flow Cyt, IP
    • Reacts with: Species independent
    • Isotype: IgG1

    製品の概要

    • 製品名

      Anti-Phosphotyrosine antibody [IG2]
      Phosphotyrosine 一次抗体 製品一覧

    • 製品の詳細

      Mouse monoclonal [IG2] to Phosphotyrosine

    • 由来種

      Mouse

    • 特異性

      ab5592 detects phosphotyrosine from most species and tissues. ab5592 will not react with any other phosphorylated amino acids, proteins or nucleotides.

    • アプリケーション

      適用あり: ELISA, ICC/IF, WB, ICC, Flow Cyt, IPmore details

    • 種交差性

      交差種: Species independent

    • 免疫原

      Chemical/ Small Molecule corresponding to Phosphotyrosine. O-phospho-L-tyrosine and O-phospho-DL-tyramine.

    • ポジティブ･コントロール

      • HeLa cells.

    • 特記事項

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    製品の特性

    アプリケーション

    Our Abpromise guarantee covers the use of ab5592 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    ELISA Use at an assay dependent concentration.
    ICC/IF 1/50 - 1/200.
    WB 1/100 - 1/1000.
    ICC 1/50 - 1/200.
    Flow Cyt Use at an assay dependent concentration.

    ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

     
    IP Use a concentration of 2 µg/ml.

    ターゲット情報

    • 関連性

      The phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function.

    画像

    • Immunocytochemistry/ Immunofluorescence - Anti-Phosphotyrosine antibody [IG2] (ab5592)
      Immunocytochemistry/ Immunofluorescence - Anti-Phosphotyrosine antibody [IG2] (ab5592)
      Immunolocalization of phosphotyrosine in HeLa cells using ab5592.

    参考文献 (0)

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