Key features and details
- Rabbit polyclonal to Phosphotyrosine (Biotin)
- Suitable for: WB, IP, ELISA
- Reacts with: Species independent
- Conjugation: Biotin
- Isotype: IgG
製品名Anti-Phosphotyrosine antibody (Biotin)
Phosphotyrosine 一次抗体 製品一覧
製品の詳細Rabbit polyclonal to Phosphotyrosine (Biotin)
特異性Recognizes both free phosphotyrosine (pY) and tyrosine-phosphorylated peptide and proteins.
アプリケーション適用あり: WB, IP, ELISAmore details
種交差性交差種: Species independent
Chemical/ Small Molecule conjugated to KLH.
- Use Vanadium treated cell culture (30 min) for Western Blotting and Tyrosine-phosphorylated peptide for ELISA.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
バッファーPreservative: 0.01% Sodium azide
Concentration information loading...
精製度Immunogen affinity purified
特記事項（精製）Immunoaffinity chromatography with phosphotyrosine-agarose.
Our Abpromise guarantee covers the use of ab9331 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at 16µg/mg of lysate. Acetone precipitation of the protein extract followed by SDS denaturation is recommended.
WB: 1/125. Not recommended for WB of trace cellular phospho-protein. Acetone precipitation of the protein extract followed by SDS denaturation is recommended.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
関連性The phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function.