Application
Western blot
Sample
Human Cell lysate - whole cell ((TF-1 (human, lymphoid, erythroleukemia) cells))
Specification
(TF-1 (human, lymphoid, erythroleukemia) cells)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3%
Other product details
Dilution
1/1000
Incubation time
1 hour(s) and 0 minute(s)
Secondary antibody
Name
Abcam antibody:Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP)
Detection
Detection method
ECL
Exposure
15 second(s)
Bands
Specific: several kDa
Additional data
Additional Notes
Very strong Ab, as with many phosphotyrosine Abs, limiting the background is difficult, but it does produce several clear bands. Used with PVDF membrane
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Alison Taylor
Verified customer
投稿 Sep 25 2006