Anti-Phosphotyrosine 抗体 (ab9319)
Key features and details
- Rabbit polyclonal to Phosphotyrosine
- Suitable for: ELISA, IP, WB, IHC-P, IHC-Fr
- Reacts with: Species independent
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Phosphotyrosine antibody
Phosphotyrosine 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Phosphotyrosine -
由来種
Rabbit -
特異性
Recognize proteins and peptides phosphorylated on tyrosine residues. No cross-reaction with phosphoserine and threonine. -
アプリケーション
適用あり: ELISA, IP, WB, IHC-P, IHC-Frmore details -
種交差性
交差種: Species independent -
免疫原
Chemical/ Small Molecule corresponding to Phosphotyrosine conjugated to keyhole limpet haemocyanin. Phospho-tyrosine KLH conjugates
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ポジティブ･コントロール
- Use Vanadium treated cell culture (30 min) for Western Blotting and Tyrosine-phosphorylated peptide for ELISA. Human breast cancer tissue
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 6.00
Preservative: 0.01% Sodium azide
Constituent: 0.114% Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Affinity purified with phospho-tyrosine on agarose and competitively eluted with N-acetylated phospho-tyrosine. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab9319の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISA |
1/1000.
|
|
| IP |
Use at an assay dependent concentration. 10 µg/500 µg protein sample.
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| WB | (2) |
1/250.
|
| IHC-P |
1/50.
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| IHC-Fr |
1/50.
|
| 特記事項 |
|---|
|
ELISA
1/1000. |
|
IP
Use at an assay dependent concentration. 10 µg/500 µg protein sample. |
|
WB
1/250. |
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IHC-P
1/50. |
|
IHC-Fr
1/50. |
ターゲット情報
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関連性
The phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function.
画像
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Western blot - Anti-Phosphotyrosine antibody (ab9319)All lanes : Anti-Phosphotyrosine antibody (ab9319) at 0.5 µg/ml
Lane 1 : 5 ul of crude protein from of HeLa cell lysate treated with fetal calf serum
Lane 2 : 15 ul of crude protein from HeLa cell lysate treated with fetal calf serum
Lane 3 : 5 ul of crude protein from HeLa cell lysate
Lane 4 : 15 ul of crude protein from HeLa cell lysate
Lysates/proteins at 2 mg/ml per lane. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phosphotyrosine antibody (ab9319)Immunohistochemistry analysis of human breast cancer with ab9319
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab9319 は 9 報の論文で使用されています。
- Verheyen T et al. Spatial organization-dependent EphA2 transcriptional responses revealed by ligand nanocalipers. Nucleic Acids Res 48:5777-5787 (2020). PubMed: 32352518
- Ramani K et al. Role of A-Kinase Anchoring Protein Phosphorylation in Alcohol-Induced Liver Injury and Hepatic Stellate Cell Activation. Am J Pathol 188:640-655 (2018). PubMed: 29305319
- Duan ZZ et al. Protease activated receptor 1 (PAR1) enhances Src-mediated tyrosine phosphorylation of NMDA receptor in intracerebral hemorrhage (ICH). Sci Rep 6:29246 (2016). PubMed: 27385592
- Yosef E et al. Do Src Kinase and Caveolin Interact Directly with Na,K-ATPase? J Biol Chem 291:11736-50 (2016). PubMed: 27022017
- Nakamura S et al. Novel roles for LIX1L in promoting cancer cell proliferation through ROS1-mediated LIX1L phosphorylation. Sci Rep 5:13474 (2015). PubMed: 26310847
- Shaw A et al. Spatial control of membrane receptor function using ligand nanocalipers. Nat Methods 11:841-6 (2014). PubMed: 24997862
- Debreuil J et al. Molecular Cloning and Characterization of First Organic Matrix Protein from Sclerites of Red Coral, Corallium rubrum. J Biol Chem 287:19367-76 (2012). WB . PubMed: 22505718
- Zhong Z et al. Protein S protects neurons from excitotoxic injury by activating the TAM receptor Tyro3-phosphatidylinositol 3-kinase-Akt pathway through its sex hormone-binding globulin-like region. J Neurosci 30:15521-34 (2010). IP . PubMed: 21084607
- Moya A et al. Carbonic anhydrase in the scleractinian coral Stylophora pistillata: characterization, localization, and role in biomineralization. J Biol Chem 283:25475-84 (2008). WB . PubMed: 18617510