Application
Western blot
Sample
Human Cell lysate - whole cell (A431)
Loading amount
1e+006 cells
Specification
A431
Treatment
calyculin A 1 h
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Other product details
Dilution
1/250
Incubation time
1 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: Amersham
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ecl
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 50, 100, 110 kDa
Positive control
calyculin A treated A431 cells
Negative control
untreated A341
Additional data
Additional Notes
seems to recognize few calyculin A (phosphatase inhibitor) induced bands; immunoprecip. may improce the result
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投稿 Dec 15 2006