Key features and details
- Rabbit polyclonal to Phosphoserine (HRP)
- Suitable for: WB, ELISA
- Reacts with: Species independent
- Conjugation: HRP
- Isotype: IgG
製品名Anti-Phosphoserine antibody (HRP)
Phosphoserine 一次抗体 製品一覧
製品の詳細Rabbit polyclonal to Phosphoserine (HRP)
特異性Specifically recognizes both free-phosphoserine, serine-phosphorylated peptide and proteins. Does not cross-react with ATP, phosphotyrosine, peptidyl phosphothreonine and serine. Has slight cross-reactivity with free phosphothreonine. Readily reacts to known phosphoproteins such as phosvitin and alpha casein.
アプリケーション適用あり: WB, ELISAmore details
種交差性交差種: Species independent
BSA and KLH-phosphoserine conjugates.
- Use Mouse brain extract for Western Blotting or synthetic phosphopeptide or phosvitin for ELISA.
保存方法Shipped at 4°C. Store at +4°C.
Constituent: Tris buffered saline
Concentration information loading...
精製度Immunogen affinity purified
特記事項（精製）Immunoaffinity chromatography with phosphoserine-agarose.
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
- 450 nm Stop Solution for TMB Substrate (ab171529)
- 650 nm Stop Solution for TMB Substrate (ab171531)
- Immunoassay Blocking Buffer (ab171534)
- Immunoassay Blocking (BSA Free) (ab171535)
Our Abpromise guarantee covers the use of ab9334 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 2 µg/ml. To block use 3%BSA with 0.1% gelatin (do not use milk). We recommend that the antibody solution should contain 0.5% BSA to prevent non-specific binding.|
|ELISA||Use a concentration of 1 µg/ml.|
関連性Changes in the serine/threonine phosphorylation state of a protein in response to various external stimuli can have profound effects on cellular signal transduction, apoptosis and carcinogenesis. The reagents, including phosphorylated protein/peptides, antibodies against the phosphospecific amino acid, are important tools to explore the activation of serine, threonine or tyrosine containing proteins. An aberrant protein phosphorylation is a hallmark of human disease, and the enzymes, particularly protein kinases, which control protein phosphorylation are recognized as a major new drug target family.
- phospho-Ser antibody
- pS antibody
- pSER antibody
ab9334 は 9 報の論文で使用されています。
- Martínez-Florensa M et al. Serine residues in the LAT adaptor are essential for TCR-dependent signal transduction. J Leukoc Biol 89:63-73 (2011). WB . PubMed: 20940326
- Zang ZJ et al. Identification of PP2A as a novel interactor and regulator of TRIP-Br1. Cell Signal 21:34-42 (2009). WB . PubMed: 18940248
- Selvarajoo K et al. Signaling flux redistribution at toll-like receptor pathway junctions. PLoS One 3:e3430 (2008). WB . PubMed: 18927610
- Bokui N et al. Involvement of MAPK signaling molecules and Runx2 in the NELL1-induced osteoblastic differentiation. FEBS Lett 582:365-71 (2008). PubMed: 18082140
- McLaughlin NJ et al. Platelet-activating factor-mediated endosome formation causes membrane translocation of p67phox and p40phox that requires recruitment and activation of p38 MAPK, Rab5a, and phosphatidylinositol 3-kinase in human neutrophils. J Immunol 180:8192-203 (2008). WB . PubMed: 18523285
- Mitchell JE et al. The presumptive phosphatidylserine receptor is dispensable for innate anti-inflammatory recognition and clearance of apoptotic cells. J Biol Chem 281:5718-25 (2006). WB . PubMed: 16317002
- Virok DP et al. Chlamydial infection induces pathobiotype-specific protein tyrosine phosphorylation in epithelial cells. Infect Immun 73:1939-46 (2005). WB . PubMed: 15784533
- Scott GK et al. Vitamin K3 (menadione)-induced oncosis associated with keratin 8 phosphorylation and histone H3 arylation. Mol Pharmacol 68:606-15 (2005). PubMed: 15939799
- Luettich K & Schmidt C TGFbeta1 activates c-Jun and Erk1 via alphaVbeta6 integrin. Mol Cancer 2:33 (2003). PubMed: 14572313