Anti-Phosphoserine Aminotransferase 抗体 (ab96136)
Key features and details
- Rabbit polyclonal to Phosphoserine Aminotransferase
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Phosphoserine Aminotransferase antibody
Phosphoserine Aminotransferase 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Phosphoserine Aminotransferase -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Rabbit, Cow -
免疫原
Recombinant protein fragment with a sequence corresponding to a region within amino acids 48 and 298 of Human Phosphoserine Aminotransferase (NP_478059).
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ポジティブ・コントロール
- 293T, A431, H1299, HeLa, HepG2, Molt-4 and Raji cell lines. H1299 whole cell lysate for WB and A549 cells for IF.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab96136の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (2) |
1/500 - 1/3000. Predicted molecular weight: 40 kDa.
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ICC/IF |
1/100 - 1/200.
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特記事項 |
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WB
1/500 - 1/3000. Predicted molecular weight: 40 kDa. |
ICC/IF
1/100 - 1/200. |
ターゲット情報
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機能
Catalyzes the reversible conversion of 3-phosphohydroxypyruvate to phosphoserine and of 3-hydroxy-2-oxo-4-phosphonooxybutanoate to phosphohydroxythreonine. -
組織特異性
Expressed at high levels in the brain, liver, kidney and pancreas, and very weakly expressed in the thymus, prostate, testis and colon. -
パスウェイ
Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 2/3.
Cofactor biosynthesis; pyridoxine 5'-phosphate biosynthesis; pyridoxine 5'-phosphate from D-erythrose 4-phosphate: step 3/5. -
関連疾患
Defects in PSAT1 are the cause of phosphoserine aminotransferase deficiency (PSATD) [MIM:610992]. PSATD is characterized biochemically by low plasma and cerebrospinal fluid concentrations of serine and glycine and clinically by intractable seizures, acquired microcephaly, hypertonia, and psychomotor retardation. -
配列類似性
Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. SerC subfamily. - Information by UniProt
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参照データベース
- Entrez Gene: 29968 Human
- Entrez Gene: 107272 Mouse
- Entrez Gene: 293820 Rat
- Omim: 610936 Human
- SwissProt: Q9Y617 Human
- SwissProt: Q99K85 Mouse
- Unigene: 494261 Human
- Unigene: 592595 Human
see all -
別名
- EC 2.6.1.52 antibody
- Endometrial progesterone induced protein antibody
- EPIP antibody
see all
画像
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (15)
ab96136 は 15 報の論文で使用されています。
- Tajan M et al. Serine synthesis pathway inhibition cooperates with dietary serine and glycine limitation for cancer therapy. Nat Commun 12:366 (2021). PubMed: 33446657
- Zhou X et al. Coordinated pyruvate kinase activity is crucial for metabolic adaptation and cell survival during mitochondrial dysfunction. Hum Mol Genet 30:2012-2026 (2021). PubMed: 34169315
- Hennequart M et al. The impact of physiological metabolite levels on serine uptake, synthesis and utilization in cancer cells. Nat Commun 12:6176 (2021). PubMed: 34702840
- Spillier Q et al. Structure-Activity Relationships (SARs) of a-Ketothioamides as Inhibitors of Phosphoglycerate Dehydrogenase (PHGDH). Pharmaceuticals (Basel) 13:N/A (2020). PubMed: 31979167
- Duan W & Liu X PSAT1 Upregulation Contributes to Cell Growth and Cisplatin Resistance in Cervical Cancer Cells via Regulating PI3K/AKT Signaling Pathway. Ann Clin Lab Sci 50:512-518 (2020). PubMed: 32826249