Application
Western blot
Sample
Bos taurus Cell lysate - whole cell (Peripheral blood mononuclear cells (PBMCs))
Gel Running Conditions
Non-reduced Denaturing (8%)
Loading amount
50 µg
Treatment
1ug/ml LPS, or 1.5mM FFA for 1hr
Specification
Peripheral blood mononuclear cells (PBMCs)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% skim milk
Dilution
1/100
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Non-Abcam antibody was used: Goat Anti-Rabbit IgG
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Negative control
(A) control (no treatment, 1hr cell cultured)
Exposure
40 minute(s) and 0 second(s)
Bands
Specific: 135-150 kDa
Positive control
(B) lipopolysaccharide 1ug/ml
(C) FFA 1.5mM
(D) both (LPS 1ug/ml + FFA 1.5mM)
1hr cell cultured
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Wang Chien Kai
Verified customer
投稿 Dec 07 2021