Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y775) 抗体 (ab41434)
Key features and details
- Rabbit polyclonal to Phospholipase C gamma 1/PLC-gamma-1 (phospho Y775)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y775) antibody
Phospholipase C gamma 1/PLC-gamma-1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Phospholipase C gamma 1/PLC-gamma-1 (phospho Y775) -
由来種
Rabbit -
特異性
Phospho-Tyr775-\Phospholipase C gamma 1/PLC-gamma-1 specific. Immunogen sequence has low homology to Phospholipase C gamma 2.
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アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide corresponding to Human Phospholipase C gamma 1/PLC-gamma-1 (phospho Y775).
Database link: P19174 -
ポジティブ・コントロール
- Human Jurkat and A431 cells treated with pervanadate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
This antibody was cross-adsorbed to a non-specific phospho-tyrosine peptide, then affinity purified using phospho- Phospholipase C gamma 1/PLC-gamma-1 (Tyr775) peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab41434の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000. Detects a band of approximately 150 kDa (predicted molecular weight: 150 kDa).
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特記事項 |
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WB
1/1000. Detects a band of approximately 150 kDa (predicted molecular weight: 150 kDa). |
ターゲット情報
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機能
Plays a role in actin reorganization and cell migration. The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. -
配列類似性
Contains 1 C2 domain.
Contains 1 EF-hand domain.
Contains 2 PH domains.
Contains 1 PI-PLC X-box domain.
Contains 1 PI-PLC Y-box domain.
Contains 2 SH2 domains.
Contains 1 SH3 domain. -
ドメイン
The SH3 domain mediates interaction with CLNK (By similarity). The SH3 domain also mediates interaction with RALGPS1. -
翻訳後修飾
The receptor-mediated activation of PLC-gamma-1 and PLC-gamma-2 involves their phosphorylation by tyrosine kinases in response to ligation of a variety of growth factor receptors and immune system receptors. May be dephosphorylated by PTPRJ.
Ubiquitinated by CBLB in activated T-cells. -
細胞内局在
Cell projection > lamellipodium. Cell projection > ruffle. Rapidly redistributed to ruffles and lamellipodia structures in response to epidermal growth factor (EGF) treatment. - Information by UniProt
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参照データベース
- Entrez Gene: 5335 Human
- Entrez Gene: 18803 Mouse
- Entrez Gene: 25738 Rat
- Omim: 172420 Human
- SwissProt: P19174 Human
- SwissProt: Q62077 Mouse
- SwissProt: P10686 Rat
- Unigene: 268177 Human
see all -
別名
- 1 phosphatidyl D myo inositol 4 5 bisphosphate antibody
- 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 1 antibody
- 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1 antibody
see all
画像
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Western blot analysis of Phospholipase C gamma 1/PLC-gamma-1 immunoprecipitates from human jurkat cells untreated (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 min (lanes 2, 4, 5 & 6). Immunoprecipitation was performed with monoclonal anti-Phospholipase C gamma 1/PLC-gamma-1. The blots were probed with anti- Phospholipase C gamma 1/PLC-gamma-1 (lanes 1 & 2) and anti-Phospholipase C gamma 1/PLC-gamma-1 (Tyr-775) (lanes 3-6). The latter antibody was used in the presence of phospho- Phospholipase C gamma 1/PLC-gamma-1 (Tyr-775) peptide (lane 5), or a non-specific phosphotyrosine peptide (lane 6).
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab41434 は論文での使用が確認できていません。