Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5951] to Phospholipase C beta 3/PLCB3 - BSA and Azide free
- Suitable for: WB, IHC-P
- Reacts with: Human
製品名Anti-Phospholipase C beta 3/PLCB3 antibody [EPR5951] - BSA and Azide free
Phospholipase C beta 3/PLCB3 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EPR5951] to Phospholipase C beta 3/PLCB3 - BSA and Azide free
アプリケーション適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt,ICC or IP
Synthetic peptide within Human Phospholipase C beta 3/PLCB3 aa 1150-1250. The exact sequence is proprietary.
ab247988 is the carrier-free version of ab124735 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab247988 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information. This product was previously labelled as Phospholipase C beta 3
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Concentration information loading...
Our Abpromise guarantee covers the use of ab247988 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 150 kDa (predicted molecular weight: 139 kDa).|
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
For antigen retrieval, heat up to 98 degree C, below boiling, and then let cool for 10-20 minutes.
機能The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.
配列類似性Contains 1 C2 domain.
Contains 1 PI-PLC X-box domain.
Contains 1 PI-PLC Y-box domain.
- Information by UniProt
- 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase beta 3 antibody
- 1-phosphatidylinositol-4 antibody
- 5-bisphosphate phosphodiesterase beta-3 antibody
This data was developed using ab124735, the same antibody clone in a different buffer formulation.
ab124735 at 1/100 dilution staining Phospholipase C beta 3/PLCB3 in paraffin-embedded Human colon tissue by Immunohistochemistry.Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.