Anti-Cytosolic Phospholipase A2 抗体 - N-terminal (ab227246)
Key features and details
- Rabbit polyclonal to Cytosolic Phospholipase A2 - N-terminal
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Cytosolic Phospholipase A2 antibody - N-terminal
Cytosolic Phospholipase A2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Cytosolic Phospholipase A2 - N-terminal -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IHC-P, WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Rabbit, Cow -
免疫原
Recombinant fragment within Human Cytosolic Phospholipase A2 (N terminal). The exact sequence is proprietary.
Database link: P47712 -
ポジティブ・コントロール
- IHC-P: Mouse duodenum and liver tissues; U87 xenograft tissue. WB: NIH/3T3 and Raji whole cell lysates. ICC/IF: HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab227246の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
1/100 - 1/1000.
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IHC-P |
1/100 - 1/1000.
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WB |
1/500 - 1/3000. Predicted molecular weight: 85 kDa.
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特記事項 |
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ICC/IF
1/100 - 1/1000. |
IHC-P
1/100 - 1/1000. |
WB
1/500 - 1/3000. Predicted molecular weight: 85 kDa. |
ターゲット情報
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機能
Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Together with its lysophospholipid activity, it is implicated in the initiation of the inflammatory response. -
組織特異性
Expressed in various tissues such as macrophages, platelets, neutrophils, fibroblasts and lung endothelium. -
配列類似性
Contains 1 C2 domain.
Contains 1 PLA2c domain. -
ドメイン
The N-terminal C2 domain associates with lipid membranes upon calcium binding. It modulates enzyme activity by presenting the active site to its substrate in response to elevations of cytosolic Ca(2+). -
翻訳後修飾
Activated by phosphorylation at both Ser-505 and Ser-727. -
細胞内局在
Cytoplasm. Cytoplasmic vesicle. Translocates to membrane vesicles in a calcium-dependent fashion. - Information by UniProt
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参照データベース
- Entrez Gene: 525072 Cow
- Entrez Gene: 151056 Human
- Entrez Gene: 5321 Human
- Entrez Gene: 18783 Mouse
- Entrez Gene: 665270 Mouse
- Entrez Gene: 100008748 Rabbit
- Entrez Gene: 192259 Rat
- Omim: 600522 Human
see all -
別名
- Calcium dependent phospholipid binding protein antibody
- CPLA 2 antibody
- cPLA2 alpha antibody
see all
画像
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Anti-Cytosolic Phospholipase A2 antibody - N-terminal (ab227246) at 1/1000 dilution + NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg
Predicted band size: 85 kDa7.5% SDS-PAGE gel.
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Paraffin-embedded mouse duodenum tissue stained for Cytosolic Phospholipase A2 using ab227246 at 1/500 dilution in immunohistochemical analysis.
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Paraffin-embedded mouse liver tissue stained for Cytosolic Phospholipase A2 using ab227246 at 1/500 dilution in immunohistochemical analysis.
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Paraffin-embedded U87 xenograft tissue stained for Cytosolic Phospholipase A2 using ab227246 at 1/500 dilution in immunohistochemical analysis.
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Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Cytosolic Phospholipase A2 (green) using ab227246 at 1/200 dilution in ICC/IF.
Lower panel: Merged with DNA probe.
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Anti-Cytosolic Phospholipase A2 antibody - N-terminal (ab227246) at 1/1000 dilution + Raji (human Burkitt's lymphoma cell line) whole cell lysate at 30 µg
Predicted band size: 85 kDa7.5% SDS-PAGE gel.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab227246 は論文での使用が確認できていません。