Application
Western blot
Sample
Pig Tissue lysate - whole (Heart)
Loading amount
7.5 µg
Specification
Heart
Gel Running Conditions
Reduced Denaturing (14% PAGE)
Blocking step
membrane blocking solution (Invitrogen) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Other product details
Dilution
1/10000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: TBST
Secondary antibody
Name
Non-Abcam antibody was used: DAKO P0448
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
ECL Prime
Exposure
45 second(s)
Bands
Specific: 6.24 kDa
Additional data
PubMed ID
N/A: View
Additional Notes
Discount code # 41824 applied to this review
lane 1 & 2: lysate from different area of frozen pig heart tissue
lane 1 & 2: lysate from different area of frozen pig heart tissue
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Dec 19 2012