Anti-Phospholamban (phospho S16) 抗体 (ab15000)
Key features and details
- Rabbit polyclonal to Phospholamban (phospho S16)
- Suitable for: IHC-Fr, IHC-P, WB
- Reacts with: Mouse, Rat, Rabbit, Hamster, Cow, Human, Pig, Chinese hamster
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Phospholamban (phospho S16) antibody
Phospholamban 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Phospholamban (phospho S16) -
由来種
Rabbit -
特異性
Multiple bands can be observed in addition to the 25kD pentamer for PLB. Monomeric, dimeric, trimeric forms, etc. can be observed if the preparations are boiled before SDS-PAGE. This antibody also can display non-specific bands at 75 and 100kD in some lysate preparations. Note: Do not boil cardiac preparation. Boiling may reduce the 25kD pentamer in to tri-, di- or monomers. In addition, phosphorylation may disrupt the pentamer into dimers and monomers. -
アプリケーション
適用あり: IHC-Fr, IHC-P, WBmore details -
種交差性
交差種: Mouse, Rat, Rabbit, Hamster, Cow, Human, Pig, Chinese hamster
交差が予測される動物種: Dog -
免疫原
Synthetic peptide corresponding to Human Phospholamban aa 1-100 (phospho S16) conjugated to keyhole limpet haemocyanin.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: 0.368% Tris glycine, 30% Glycerol, 0.87% Sodium chloride -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab15000の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-Fr | (1) |
Use at an assay dependent concentration.
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IHC-P | (1) |
Use at an assay dependent concentration.
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WB | (8) |
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 6.24 kDa.
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特記事項 |
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IHC-Fr
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 6.24 kDa. |
ターゲット情報
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機能
Reversibly inhibits the activity of ATP2A2 in cardiac sarcoplasmic reticulum by decreasing the apparent affinity of the ATPase for Ca(2+). Modulates the contractility of the heart muscle in response to physiological stimuli via its effects on ATP2A2. Modulates calcium re-uptake during muscle relaxation and plays an important role in calcium homeostasis in the heart muscle. The degree of ATP2A2 inhibition depends on the oligomeric state of PLN. ATP2A2 inhibition is alleviated by PLN phosphorylation. -
組織特異性
Heart muscle (at protein level). -
関連疾患
Cardiomyopathy, dilated 1P
Cardiomyopathy, familial hypertrophic 18 -
配列類似性
Belongs to the phospholamban family. -
翻訳後修飾
Phosphorylation by PKA abolishes the inhibition of ATP2A2-mediated calcium uptake. Phosphorylated at Thr-17 by CaMK2, and in response to beta-adrenergic stimulation. Phosphorylation by DMPK may stimulate sarcoplasmic reticulum calcium uptake in cardiomyocytes. -
細胞内局在
Endoplasmic reticulum membrane. Sarcoplasmic reticulum membrane. Mitochondrion membrane. Membrane. Colocalizes with HAX1 at the endoplasmic reticulum (PubMed:17241641). Colocalizes with DMPK a the sarcoplasmic reticulum (PubMed:15598648). - Information by UniProt
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参照データベース
- Entrez Gene: 5350 Human
- Entrez Gene: 18821 Mouse
- Entrez Gene: 64672 Rat
- Omim: 172405 Human
- SwissProt: A4IFH6 Cow
- SwissProt: P61012 Dog
- SwissProt: P26678 Human
- SwissProt: P61014 Mouse
see all -
別名
- Cardiac phospholamban antibody
- CMD1P antibody
- CMH18 antibody
see all
画像
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (33)
ab15000 は 33 報の論文で使用されています。
- Benkner A et al. Riociguat attenuates the changes in left ventricular proteome and microRNA profile after experimental aortic stenosis in mice. Br J Pharmacol 179:4575-4592 (2022). PubMed: 35751875
- Li H et al. Notoginsenoside R1-loaded mesoporous silica nanoparticles targeting the site of injury through inflammatory cells improves heart repair after myocardial infarction. Redox Biol 54:102384 (2022). PubMed: 35777198
- Wang W et al. RETRACTED ARTICLE: Inhibition of miR-296-5p protects the heart from cardiac hypertrophy by targeting CACNG6. Gene Ther 28:391 (2021). PubMed: 31844153
- Xu D et al. Xanthine oxidase inhibitor febuxostat reduces atrial fibrillation susceptibility by inhibition of oxidized CaMKII in Dahl salt-sensitive rats. Clin Sci (Lond) 135:2409-2422 (2021). PubMed: 34386810
- Zheng J et al. Stachydrine hydrochloride suppresses phenylephrine-induced pathological cardiac hypertrophy by inhibiting the calcineurin/nuclear factor of activated T-cell signalling pathway. Eur J Pharmacol 883:173386 (2020). PubMed: 32712088