製品の概要

  • 製品名

    Phosphoglucose Isomerase Activity Assay Kit (Colorimetric)
    Glucose 6 Phosphate Isomerase キット 製品一覧
  • 検出方法

    Colorimetric
  • サンプルの種類

    Tissue, Adherent cells, Suspension cells, Tissue Homogenate
  • アッセイタイプ

    Enzyme activity
  • 検出感度

    < 0.1 mU/well
  • 検出範囲

    2.5 nmol/well - 12.5 nmol/well
  • 種交差性

    交差種: Mammals, Other species
  • 製品の概要

    Abcam’s Phosphoglucose Isomerase Activity Assay Kit (Colorimetric) (ab155897) is an assay where PGI (phosphoglucose isomerase) converts fructose-6-phosphate to glucose-6-phosphate; the glucose-6-phosphate is oxidized by glucose-6-phosphate dehydrogenase to form a product, which reacts with a colorless probe to give strong absorbance at 450 nm. The PGI assay is simple, sensitive and rapid and can detect phosphoglucose isomerase activity less than 0.1 mU/reaction.

  • 特記事項

    Phosphoglucose isomerase (glucose-6-phosphate isomerase or PGI, EC 5.3.1.9) is an important housekeeping enzyme. PGI catalyzes the interconversion of glucose-6- phosphate to fructose-6-phosphate. PGI performs multiple functions & intracellularly plays key role in both glycolysis and gluconeogenesis. Extracellularly, PGI [also called Autocrine Motility Factor (AMF)] functions as a cytokine, which stimulates cell motility and is associated with tumor development and metastasis. In humans, PGI deficiency causes hemolytic anemia, whereas increased PGI activity is observed in many cancers such as gastrointestinal, kidney and breast cancer. Early detection of abnormal phosphoglucose isomerase activity is crucial for diagnosis, prediction and therapeutic strategy.

  • 試験プラットフォーム

    Microplate reader

製品の特性

画像

  • NADH standard curve generated useing the method described in the protocol booklet. 

     

  • Phosphoglucose Isomerase activity in bovine liver, HeLa cell lysate and positive control

プロトコール

参考文献

ab155897 has not yet been referenced specifically in any publications.

レビューと Q&A

Following ratios were analysed.
Protein X 1:5 Phosphoglucose Isomerase enzyme
Protein X 1:4 Phosphoglucose Isomerase enzyme
Protein X 1:3 Phosphoglucose Isomerase enzyme
Protein X 1:2 Phosphoglucose Isomerase enzyme
Protein X 1:1 Phosphoglucose Isomerase enzyme
Protein X 2:1 Phosphoglucose Isomerase enzyme
Protein X 3:1 Phosphoglucose Isomerase enzyme
Protein X 4:1 Phosphoglucose Isomerase enzyme
Protein X 5:1 Phosphoglucose Isomerase enzyme
Phosphoglucose isomerase activity
1:5 -1,26859
1:4 -2,05864
1:3 -1,79833
1:2 -2,39248
1:1 -1,2912
2:1 -2,80196
3:1 -1,72374
4:1 -2,97389
5:1 -2,36312
Our results indicate a downward trend (inhibitory effect) to be present of our protein towards the PGI activity. However, as some kind of parallel lines popped up from the data, we would consider the data inconclusive as such. A re-analysis would be necessary.
But, as the positive control can only be reconstituted with 20 µl of assay buffer, we think the kit is not suitable for activation-inhibition experiments at the moment.

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投稿 Sep 11 2018

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