Phenylalanine Assay Kit (ab83376)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 2 µM
製品の概要
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製品名
Phenylalanine Assay Kit -
検出方法
Fluorescent -
サンプルの種類
Urine, Serum, Plasma, Other biological fluids, Tissue Extracts -
アッセイタイプ
Quantitative -
検出感度
> 2 µM -
検出範囲
2 µM - 20 µM -
全工程の試験時間
1h 00m -
製品の概要
Phenylalanine Assay Kit ab83376 provides a quick, simple, accurate method for quantifying phenylalanine in biological samples.
In the phenylalanine assay protocol, phenylalanine is reductively deaminated with the simultaneous formation of NADH which reacts with our fluorescent probe to generate fluorescence at Ex/Em=535/587 nm. The assay is linear in the range from 0.1 to 1.0 nmol (2-20 µM) of Phenylalanine.
Phenylalanine assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 60 min
- analyze with a microplate reader -
特記事項
This product is manufactured by BioVision, an Abcam company and was previously called K572 Phenylalanine Fluorometric Assay Kit. K572-100 is the same size as the 100 test size of ab83376.
L-Phenylalanine (PHE) is an electrically-neutral amino acid, one of the twenty common and one of the three aromatic amino acids used to biochemically form proteins. Phenylalanine uses the same active transport channel as tryptophan to cross the blood-brain barrier, and, in large quantities, interferes with the production of serotonin.
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 100 tests Phenyalanine Standard 1 vial Fructose Assay Buffer 1 x 25ml Phenylalanine Developer Mix 1 vial Phenylalanine Enzyme Mix 1 vial Tyrosinase 1 vial -
研究分野
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関連性
L-Phenylalanine (PHE) is an electrically-neutral amino acid, one of the twenty common and one of the three aromatic amino acids used to biochemically form proteins. Phenylalanine uses the same active transport channel as tryptophan to cross the blood-brain barrier, and, in large quantities, interferes with the production of serotonin. Errors in PHE metabolism lead to phenylketonuria or PKU which can have dire consequences. -
別名
- Phe
関連製品
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データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab83376 は論文での使用が確認できていません。