遺伝子ノックアウト細胞株 検証済
リコンビナント
RabMAb

Anti-PHD2 / prolyl hydroxylase 抗体 [EPR3660(B)(2)] (ab133630)

製品の概要

  • 製品名
    Anti-PHD2 / prolyl hydroxylase antibody [EPR3660(B)(2)]
    PHD2 / prolyl hydroxylase 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR3660(B)(2)] to PHD2 / prolyl hydroxylase
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WB, IPmore details
    適用なし: ICC or IHC-P
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide within Human PHD2/ prolyl hydroxylase (N terminal). The exact sequence is proprietary.

  • ポジティブ・コントロール
    • SH-SY5Y treated with cobalt chloride, SH-SY5Y, and Human adrenal gland lysates
  • 特記事項

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab133630 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 46 kDa.
IP 1/10 - 1/100.
  • 追加情報
    Is unsuitable for ICC or IHC-P.
  • ターゲット情報

    • 機能
      Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. Functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex.
    • 組織特異性
      According to PubMed:11056053, widely expressed with highest levels in skeletal muscle and heart, moderate levels in pancreas, brain (dopaminergic neurons of adult and fetal substantia nigra) and kidney, and lower levels in lung and liver. According to PubMed:12351678 widely expressed with highest levels in brain, kidney and adrenal gland. Expressed in cardiac myocytes, aortic endothelial cells and coronary artery smooth muscle.
    • 関連疾患
      Defects in EGLN1 are the cause of erythrocytosis familial type 3 (ECYT3) [MIM:609820]. ECYT3 is an autosomal dominant disorder characterized by increased serum red blood cell mass, elevated serum hemoglobin and hematocrit, and normal serum erythropoietin levels.
    • 配列類似性
      Contains 1 Fe2OG dioxygenase domain.
      Contains 1 MYND-type zinc finger.
    • Information by UniProt
    • 参照データベース
    • 別名
      • C1ORF12 antibody
      • Chromosome 1 Open Reading Frame 12 antibody
      • DKFZp761F179 antibody
      • ECYT 3 antibody
      • ECYT3 antibody
      • Egl 9 family hypoxia inducible factor 1 antibody
      • EGL 9 homolog of C. elegans 1 antibody
      • EGL nine (C.elegans) homolog 1 antibody
      • Egl nine homolog 1 (C. elegans) antibody
      • Egl nine homolog 1 antibody
      • Egl nine like protein 1 antibody
      • EGLN 1 antibody
      • Egln1 antibody
      • EGLN1_HUMAN antibody
      • HIF PH2 antibody
      • HIF Prolyl Hydroxylase 2 antibody
      • HIF-PH2 antibody
      • HIF-prolyl hydroxylase 2 antibody
      • HIFP4H 2 antibody
      • HIFPH2 antibody
      • HPH 2 antibody
      • HPH-2 antibody
      • HPH2 antibody
      • Hypoxia inducible factor prolyl hydroxylase 2 antibody
      • Hypoxia-inducible factor prolyl hydroxylase 2 antibody
      • ORF13 antibody
      • P4H2 antibody
      • PHD 2 antibody
      • PhD2 antibody
      • PNAS 118 antibody
      • PNAS 137 antibody
      • Prolyl Hydroxylase Domain Containing Protein 2 antibody
      • Prolyl hydroxylase domain-containing protein 2 antibody
      • Rat Homolog of SM20 antibody
      • SM 20 antibody
      • SM-20 antibody
      • SM20 antibody
      • Zinc finger MYND domain containing protein 6 antibody
      • ZMYND6 antibody
      see all

    画像

    • Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: PHD2 / prolyl hydroxylase knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: HepG2 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab133630 observed at 1X kDa. Red - loading control, ab8245, observed at 37 kDa.

      Unpurified ab133630 was shown to specifically react with PHD2 / prolyl hydroxylase when PHD2 / prolyl hydroxylase knockout samples were used. Wild-type and PHD2 / prolyl hydroxylase knockout samples were subjected to SDS-PAGE. Unpurified ab133630 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW)  preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD)  preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • ab133630 (purified) at 1:40 dilution (2µg) immunoprecipitating PHD2 / prolyl hydroxylase in SH-SY5Y treated with 0.1mM cobalt chloride for 8 hours whole cell lysate.
      Lane 1 (input): SH-SY5Y (Human neuroblastoma epithelial cell) treated with 0.1mM cobalt chloride for 8 hours whole cell lysate 10µg
      Lane 2 (+): ab133630 & SH-SY5Y treated with 0.1mM cobalt chloride for 8 hours whole cell lysate
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab133630 in SH-SY5Y treated with 0.1mM cobalt chloride for 8 hours whole cell lysate
      For western blotting, VeriBlot for IP secondary antibody (HRP) (ab131366) was used as the secondary antibody at 1:1000 dilution.
      Blocking and diluting buffer: 5% NFDM/TBST.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • All lanes : Anti-PHD2 / prolyl hydroxylase antibody [EPR3660(B)(2)] (ab133630) at 1/5000 dilution (Purified)

      Lane 1 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysates with 5% NFDM/TBST
      Lane 2 : SH-SY5Y (Human neuroblastoma epithelial cell) treated with 0.1mM cobalt chloride for 8 hours whole cell lysates with 5% NFDM/TBST
      Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates with 5% NFDM/TBST

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 46 kDa
      Observed band size: 46 kDa

    参考文献

    This product has been referenced in:
    • Bartoszewska S  et al. The hypoxia-inducible miR-429 regulates hypoxia-inducible factor-1a expression in human endothelial cells through a negative feedback loop. FASEB J 29:1467-79 (2015). Read more (PubMed: 25550463) »

    See 1 Publication for this product

    レビューと Q&A

    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (10%)
    Sample
    Human Cell lysate - whole cell (osteosarcoma cells)
    Specification
    osteosarcoma cells
    Treatment
    lane 1 siRNA for PHD2, lane 2 control, 48h
    Blocking step
    Milk as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 21°C
    Username

    Dr. Sonia Rocha

    Verified customer

    投稿 Sep 26 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    登録