Anti-PHAP1 抗体 (ab5992)

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Western blot - Anti-PHAP1 antibody (ab5992)
  • Immunocytochemistry/ Immunofluorescence - Anti-PHAP1 antibody (ab5992)
  • Western blot - Anti-PHAP1 antibody (ab5992)
  • Immunocytochemistry/ Immunofluorescence - Anti-PHAP1 antibody (ab5992)

Key features and details

  • Rabbit polyclonal to PHAP1
  • Suitable for: ICC/IF, WB
  • Reacts with: Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-PHAP1 antibody
    PHAP1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to PHAP1

  • 由来種

    Rabbit

  • 特異性

    This antibody does not cross-react with PHAP12a or PHAPIII.

  • アプリケーション

    適用あり: ICC/IF, WBmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Rat

  • 免疫原

    Synthetic peptide corresponding to Human PHAP1.
    (Peptide available as ab6241)

  • ポジティブ･コントロール

    • WB: Daudi, HeLa, HepG2, K562 and Raji cell lysates. ICC/IF: Raji cells

  • 特記事項

    Apoptosis is related to many diseases and development. Caspase-9 plays a central role in cell death induced by a variety of apoptosis activators. Cytochrome c, after released from mitochondria, binds to Apaf-1, which forms an apoptosome that in turn binds to and activate procaspase-9. Activated caspase-9 cleaves and activates the effector caspases (caspase-3, -6 and –7), which are responsible for the proteolytic cleavage of many key proteins in apoptosis. The tumor suppressor putative HLA-DR-associated proteins (PHAPs) were recently identified as important regulators of mitochondrion apoptosis. PHAP appears to facilitate apoptosome-mediated caspase-9 activation and to stimulate the mitochondrial apoptotic pathway. PHAP was also shown to oppose both Ras- and Myc-mediated cell transformation.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab5992の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
Use a concentration of 2 µg/ml.
WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 32 kDa.
特記事項
ICC/IF
Use a concentration of 2 µg/ml.
WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 32 kDa.

ターゲット情報

  • 機能

    Implicated in a number of cellular processes, including proliferation, differentiation, caspase-dependent and caspase-independent apoptosis, suppression of transformation (tumor suppressor), inhibition of protein phosphatase 2A, regulation of mRNA trafficking and stability in association with ELAVL1, and inhibition of acetyltransferases as part of the INHAT (inhibitor of histone acetyltransferases) complex. Plays a role in E4F1-mediated transcriptional repression.

  • 組織特異性

    Expressed in all tissues tested. Highly expressed in kidney and skeletal muscle, moderate levels of expression in brain, placenta and pancreas, and weakly expressed in lung. Found in all regions of the brain examined (amygdala, caudate nucleus, corpus callosum, hippocampus and thalamus), with highest levels in amygdala.

  • 配列類似性

    Belongs to the ANP32 family.
    Contains 4 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.

  • 翻訳後修飾

    Phosphorylated on serine residues.
    The N-terminus is blocked.
    Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group.

  • 細胞内局在

    Nucleus. Cytoplasm. Endoplasmic reticulum. Translocates to the cytoplasm during the process of neuritogenesis (By similarity). Shuttles between nucleus and cytoplasm.
  • Information by UniProt

  • 参照データベース

    • 別名

      • acidic (leucine-rich) nuclear phosphoprotein 32 family, member A antibody
      • Acidic leucine-rich nuclear phosphoprotein 32 family member A antibody
      • Acidic nuclear phosphoprotein 32 family member A antibody
      • Acidic nuclear phosphoprotein pp32 antibody
      • AN32A_HUMAN antibody
      • ANP32A antibody
      • C15orf1 antibody
      • Cerebellar leucine rich acidic nuclear protein antibody
      • Hepatopoietin Cn antibody
      • HPPCn antibody
      • I1PP2A antibody
      • Inhibitor 1 of protein phosphatase 2A antibody
      • inhibitor-1 of protein phosphatase-2A antibody
      • Lanp antibody
      • Leucine rich acidic nuclear protein antibody
      • Leucine-rich acidic nuclear protein antibody
      • MAPM antibody
      • Mapmodulin antibody
      • MGC119787 antibody
      • MGC150373 antibody
      • PHAPI antibody
      • Potent heat stable protein phosphatase 2A inhibitor I1PP2A antibody
      • Potent heat-stable protein phosphatase 2A inhibitor I1PP2A antibody
      • PP32 antibody
      • Putative HLA DR associated protein I antibody
      • Putative HLA-DR-associated protein I antibody
      • Putative human HLA class II associated protein I antibody
      • Putative human HLA class II-associated protein antibody
      see all

    画像

    • Western blot - Anti-PHAP1 antibody (ab5992)
      Western blot - Anti-PHAP1 antibody (ab5992)
      All lanes : Anti-PHAP1 antibody (ab5992) at 2 µg/ml

      Lane 1 : Daudi cell lysates
      Lane 2 : HeLa cell lysates
      Lane 3 : HepG2 cell lysates
      Lane 4 : K562 cell lysates

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
    • Immunocytochemistry/ Immunofluorescence - Anti-PHAP1 antibody (ab5992)
      Immunocytochemistry/ Immunofluorescence - Anti-PHAP1 antibody (ab5992)

      Immunofluorescent analysis of 4% paraformaldehydefixed Raji Cells labeling PHAP I with ab5992 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

    • Western blot - Anti-PHAP1 antibody (ab5992)
      Western blot - Anti-PHAP1 antibody (ab5992)
      Western blot analysis of PHAP I expression in human Raji cell lysate with anti-PHAP I at 2 µg/ml (lane A) and 4 µg/ml (lane B), respectively. Western blot analysis of PHAP I expression in human Raji cell lysate with anti-PHAP I at 2 µg/ml (lane A) and 4 µg/ml (lane B), respectively.
    • Immunocytochemistry/ Immunofluorescence - Anti-PHAP1 antibody (ab5992)
      Immunocytochemistry/ Immunofluorescence - Anti-PHAP1 antibody (ab5992)

      Immunocytochemical analysis of Raji cells labeling PHAP1 with ab5992 at 2 μg/mL. Cells were fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

    参考文献 (1)

    ab5992 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab5992 は 1 報の論文で使用されています。

    • de Souza CF  et al. Creatine transporter immunolocalization in aged human and detached retinas. Invest Ophthalmol Vis Sci 53:1936-45 (2012). PubMed: 22410560

    レビューと Q&A

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    Question

    I wish to buy the anti PHAPI antibody however I cant tell what the difference is between ab5992 and ab5991. Is there any difference?

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    Answer

    Thank you for your patience in awaiting a response from us. Ab5992 and Ab5991 are both against the same protein, PHAP1, but the sequences were derived from two different locations; the intermediate domain (IN) and the C-terminus (CT) of the protein respectively.

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