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  1. Link

    phap1-antibody-ab5992.pdf

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Cell Biology Apoptosis Intracellular Caspases etc Caspases
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Anti-PHAP1 抗体 (ab5992)

  • Datasheet
Submit a review Q&A (1)References (1)

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Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Western blot - Anti-PHAP1 antibody (ab5992)
  • Immunocytochemistry/ Immunofluorescence - Anti-PHAP1 antibody (ab5992)
  • Western blot - Anti-PHAP1 antibody (ab5992)
  • Immunocytochemistry/ Immunofluorescence - Anti-PHAP1 antibody (ab5992)

Key features and details

  • Rabbit polyclonal to PHAP1
  • Suitable for: ICC/IF, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

こちらの製品もご検討ください

タンパク質
Product image
Recombinant Human PHAP1 protein (ab101155)
二次抗体
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

関連製品

製品の概要

  • 製品名

    Anti-PHAP1 antibody
    PHAP1 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to PHAP1
  • 由来種

    Rabbit
  • 特異性

    This antibody does not cross-react with PHAP12a or PHAPIII.
  • アプリケーション

    適用あり: ICC/IF, WBmore details
  • 種交差性

    交差種: Mouse, Human
    交差が予測される動物種: Rat
  • 免疫原

    Synthetic peptide corresponding to Human PHAP1.
    (Peptide available as ab6241)

  • ポジティブ・コントロール

    • WB: Daudi, HeLa, HepG2, K562 and Raji cell lysates. ICC/IF: Raji cells
  • 特記事項

    Apoptosis is related to many diseases and development. Caspase-9 plays a central role in cell death induced by a variety of apoptosis activators. Cytochrome c, after released from mitochondria, binds to Apaf-1, which forms an apoptosome that in turn binds to and activate procaspase-9. Activated caspase-9 cleaves and activates the effector caspases (caspase-3, -6 and –7), which are responsible for the proteolytic cleavage of many key proteins in apoptosis. The tumor suppressor putative HLA-DR-associated proteins (PHAPs) were recently identified as important regulators of mitochondrion apoptosis. PHAP appears to facilitate apoptosome-mediated caspase-9 activation and to stimulate the mitochondrial apoptotic pathway. PHAP was also shown to oppose both Ras- and Myc-mediated cell transformation.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
  • バッファー

    pH: 7.2
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • 一次抗体 備考

    Apoptosis is related to many diseases and development. Caspase-9 plays a central role in cell death induced by a variety of apoptosis activators. Cytochrome c, after released from mitochondria, binds to Apaf-1, which forms an apoptosome that in turn binds to and activate procaspase-9. Activated caspase-9 cleaves and activates the effector caspases (caspase-3, -6 and –7), which are responsible for the proteolytic cleavage of many key proteins in apoptosis. The tumor suppressor putative HLA-DR-associated proteins (PHAPs) were recently identified as important regulators of mitochondrion apoptosis. PHAP appears to facilitate apoptosome-mediated caspase-9 activation and to stimulate the mitochondrial apoptotic pathway. PHAP was also shown to oppose both Ras- and Myc-mediated cell transformation.
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Caspases etc
    • Caspases
    • Cell Biology
    • Apoptosis
    • Mitochondrial
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • Other
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Mitochondrial
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human PHAP1 protein (ab101155)

アプリケーション

Our Abpromise guarantee covers the use of ab5992 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 2 µg/ml.
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 32 kDa.

ターゲット情報

  • 機能

    Implicated in a number of cellular processes, including proliferation, differentiation, caspase-dependent and caspase-independent apoptosis, suppression of transformation (tumor suppressor), inhibition of protein phosphatase 2A, regulation of mRNA trafficking and stability in association with ELAVL1, and inhibition of acetyltransferases as part of the INHAT (inhibitor of histone acetyltransferases) complex. Plays a role in E4F1-mediated transcriptional repression.
  • 組織特異性

    Expressed in all tissues tested. Highly expressed in kidney and skeletal muscle, moderate levels of expression in brain, placenta and pancreas, and weakly expressed in lung. Found in all regions of the brain examined (amygdala, caudate nucleus, corpus callosum, hippocampus and thalamus), with highest levels in amygdala.
  • 配列類似性

    Belongs to the ANP32 family.
    Contains 4 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
  • 翻訳後修飾

    Phosphorylated on serine residues.
    The N-terminus is blocked.
    Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group.
  • 細胞内局在

    Nucleus. Cytoplasm. Endoplasmic reticulum. Translocates to the cytoplasm during the process of neuritogenesis (By similarity). Shuttles between nucleus and cytoplasm.
  • Target information above from: UniProt accession P39687 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 8125 Human
    • Entrez Gene: 11737 Mouse
    • Entrez Gene: 25379 Rat
    • Omim: 600832 Human
    • SwissProt: P39687 Human
    • SwissProt: O35381 Mouse
    • SwissProt: P49911 Rat
    • Unigene: 458747 Human
    • Unigene: 269088 Mouse
    • Unigene: 10123 Rat
    see all
  • 別名

    • acidic (leucine-rich) nuclear phosphoprotein 32 family, member A antibody
    • Acidic leucine-rich nuclear phosphoprotein 32 family member A antibody
    • Acidic nuclear phosphoprotein 32 family member A antibody
    • Acidic nuclear phosphoprotein pp32 antibody
    • AN32A_HUMAN antibody
    • ANP32A antibody
    • C15orf1 antibody
    • Cerebellar leucine rich acidic nuclear protein antibody
    • Hepatopoietin Cn antibody
    • HPPCn antibody
    • I1PP2A antibody
    • Inhibitor 1 of protein phosphatase 2A antibody
    • inhibitor-1 of protein phosphatase-2A antibody
    • Lanp antibody
    • Leucine rich acidic nuclear protein antibody
    • Leucine-rich acidic nuclear protein antibody
    • MAPM antibody
    • Mapmodulin antibody
    • MGC119787 antibody
    • MGC150373 antibody
    • PHAPI antibody
    • Potent heat stable protein phosphatase 2A inhibitor I1PP2A antibody
    • Potent heat-stable protein phosphatase 2A inhibitor I1PP2A antibody
    • PP32 antibody
    • Putative HLA DR associated protein I antibody
    • Putative HLA-DR-associated protein I antibody
    • Putative human HLA class II associated protein I antibody
    • Putative human HLA class II-associated protein antibody
    see all

画像

  • Western blot - Anti-PHAP1 antibody (ab5992)
    Western blot - Anti-PHAP1 antibody (ab5992)
    All lanes : Anti-PHAP1 antibody (ab5992) at 2 µg/ml

    Lane 1 : Daudi cell lysates
    Lane 2 : HeLa cell lysates
    Lane 3 : HepG2 cell lysates
    Lane 4 : K562 cell lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
  • Immunocytochemistry/ Immunofluorescence - Anti-PHAP1 antibody (ab5992)
    Immunocytochemistry/ Immunofluorescence - Anti-PHAP1 antibody (ab5992)

    Immunofluorescent analysis of 4% paraformaldehydefixed Raji Cells labeling PHAP I with ab5992 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

  • Western blot - Anti-PHAP1 antibody (ab5992)
    Western blot - Anti-PHAP1 antibody (ab5992)
    Western blot analysis of PHAP I expression in human Raji cell lysate with anti-PHAP I at 2 µg/ml (lane A) and 4 µg/ml (lane B), respectively. Western blot analysis of PHAP I expression in human Raji cell lysate with anti-PHAP I at 2 µg/ml (lane A) and 4 µg/ml (lane B), respectively.
  • Immunocytochemistry/ Immunofluorescence - Anti-PHAP1 antibody (ab5992)
    Immunocytochemistry/ Immunofluorescence - Anti-PHAP1 antibody (ab5992)

    Immunocytochemical analysis of Raji cells labeling PHAP1 with ab5992 at 2 μg/mL. Cells were fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

プロトコール

  • Western blot protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

データシートおよび資料

    • Datasheet
  • 参考文献 (1)

    ab5992 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab5992 は 1 報の論文で使用されています。

    • de Souza CF  et al. Creatine transporter immunolocalization in aged human and detached retinas. Invest Ophthalmol Vis Sci 53:1936-45 (2012). PubMed: 22410560

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Question

    I wish to buy the anti PHAPI antibody however I cant tell what the difference is between ab5992 and ab5991. Is there any difference?

    Read More

    Abcam community

    Verified customer

    Asked on Jun 24 2004

    Answer

    Thank you for your patience in awaiting a response from us. Ab5992 and Ab5991 are both against the same protein, PHAP1, but the sequences were derived from two different locations; the intermediate domain (IN) and the C-terminus (CT) of the protein respectively.

    Read More

    Abcam Scientific Support

    Answered on Jun 30 2004

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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