製品の概要

  • 製品名

    Phalloidin-iFluor 488 Reagent
    F-actin キット 製品一覧
  • サンプルの種類

    Adherent cells, Suspension cells
  • アッセイタイプ

    Cell-based (qualitative)
  • 製品の概要

    Phalloidin-iFluor 488 Reagent ab176753 is one of a series of phalloidin conjugates that bind to actin filaments, also known as F-actin. The iFluor 488 dye can be easily detected with a fluorescent microscope at Ex/Em = 493/517 nm.


    Our phalloidin conjugates are convenient probes for labeling, identifying and quantifying animal or plant actin filaments in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments. They can also be used with paraffin-embedded samples that have been de-paraffinized.


    Review other popular phalloidin dye conjugates, including Phalloidin-iFluor 647Phalloidin-iFluor 594Phalloidin-iFluor 555, and Rhodamine Phalloidin, search the website to see all phallodin conjugates, or read the phalloidin staining protocol.

  • 特記事項

    Staining fixed cell or tissue samples with phalloidin conjugates is very simple; it requires a single 20-90 min incubation with the phalloidin, followed by 3 short wash steps. Phalloidin staining can be combined with antibody-based staining by adding the phalloidin conjugate during either the primary or secondary antibody incubation step.

    When used in unfixed samples, phalloidin binding leads to a decrease in the disassociation rate of actin subunits from the ends of actin filaments, essentially stabilizing actin filaments through the prevention of filament depolymerisation.

  • 試験プラットフォーム

    Fluorescence microscope

製品の特性

画像

  • Ab176753 at 1/1000 dilution in PBS whole mount Immunofluorescence of Mouse inner ear sensory epithelia. Tissue was incubated for 2 hours at room temperature.

  • Actin filaments staining in HeLa cells. Actin filaments (green) were stained with CytoPainter Phalloidin-iFluor 488 reagent (ab176753); tubulin filaments were stained with a mouse anti-tubulin antibody/goat anti-mouse IgG (red). Nuclei were stained with Hoechst 33342.

  • Excitation and emission spectra of phalloidin-iFluor 488 reagent.

プロトコール

参考文献

This product has been referenced in:

  • Wang JN  et al. Acetyl-macrocalin B suppresses tumor growth in esophageal squamous cell carcinoma and exhibits synergistic anti-cancer effects with the Chk1/2 inhibitor AZD7762. Toxicol Appl Pharmacol 365:71-83 (2019). Read more (PubMed: 30633885) »
  • Hacohen-Kleiman G  et al. Atypical Auditory Brainstem Response and Protein Expression Aberrations Related to ASD and Hearing Loss in the Adnp Haploinsufficient Mouse Brain. Neurochem Res N/A:N/A (2019). Read more (PubMed: 30659505) »
See all 46 Publications for this product

レビューと Q&A

1-8 of 8 Abreviews or Q&A

Phalloidin-iFluor 488 Reagent

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Bronchial epithelial cell line. 4% paraformaldehyde fixation for 15min RT. Stain with Phalloidin (1:1000) for 45 min at RT and nucleus co-stained with DAPI.

Abcam user community

Verified customer

投稿 Jul 10 2019

Good staining

Good Good 4/5 (Ease of Use)
Abreviews
Epithelial cells:
- Fixed 4% PFA (room temperature)
- Dilution 1:1000
- DAPI co-staining
- Fluorescence measured with confocal microscopy

Abcam user community

Verified customer

投稿 Apr 24 2019

Whole mount IF for the inner ear

Excellent Excellent 5/5 (Ease of Use)
Abreviews
I used abcam's phalloidin-488 for whole mount IF of mouse inner ear (sensory epithelia). It stains actin so stereocilia are stained very strong. Diluted 1:1000 in PBS and incubated for 2 hours in RT.

Mr. Shahar Taiber

Verified customer

投稿 Jun 04 2018

Phalloidin immunofluorescence of lim2099 cells

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Seeding of 200.000 cells/well on cover slips in a 24-well-plate overnight.
5 min washing with PBS 0.1% Tween
20 min fixation with 4% PFA at room temperature.
3 x 5 min washing with PBS 0.1% Tween
10 min permeabilization with PBS 0.3% Triton-X 100
3 x 5 min washing with PBS 0.1% Tween.
Phalloidin staining for 1 hour at room temperature in the dark: 100 µl of 1x reagent diluted in PBS 0.1% Triton-X 100 1.5% BSA per coverslip.
3 x 5 min PBS 0.1% Tween.
Mounting of coverslips with ProLong Gold Antifade Mountant with DAPI on microscope slides.
Drying overnight at 4°C and imaging on the next day.

CytoPainter Phalloidin-iFluor 488 Reagent is an excellent stain yielding great results at low expenses in comparison to other more expensive stains from competing companies.

Abcam user community

Verified customer

投稿 Jun 29 2017

F-Actin / Peritoneal Macrophages

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Excellent product for F-Actin staining. Very bright, yields minimal background if any when used correctly. Can be used for z-stacking without losing the signal in steps.

Gokhan Yilmaz

Verified customer

投稿 Mar 31 2017

CytoPainter Phalloidin-iFluor 488

Excellent Excellent 5/5 (Ease of Use)
Abreviews
For our experiments, the macrophage J774A.1 cell line was placed in 24-well tissue culture plates containing round glass coverslips. After 24h, cells were washed four times and fixed with cold paraformaldehyde (3.2% in PBS) for 20 min at room temperature. After being fixed, the cells were permeabilized with Triton X-100 (0.1% in PBS) for 5 min at room temperature and washed four times with PBS. CytoPainter Phalloidin-iFluor 488 (1:1000 dilution in 1%BSA-PBS), which binds polymerized F-actin, was used to identify actin filaments and fibers. Coverslips were mounted on glass slides with mounting medium containing DAPI.
Preparations were examined with a Nikon A1R confocal scanning laser microscope equipped with 405nm/450nm (DAPI) and 488nm/525nm (CytoPainter iFluor) lasers. Images were captured at random with a ×20 (A), ×40 (B) or ×60 (C, D) objectives, and processed using the NIS-Elements 3.2 software. Scale bars: A, 50µm B, 10 µm.

Dr. Jose Ramos Vivas

Verified customer

投稿 Jun 24 2016

I stained the murine osteocyte cells (MLO-Y4 cells line) with Phalloidin-iFluor 488, DAPI staining and sclerostin antibody labbeled with iFluor 550. I used this protocol :
Fixed cells with PFA 4% for 20 min at room temperature
Wash 3 times for 5 min with PBS
Incubate for 10 min at room temperature with PBS-Triton 0.1%
Wash 3 times for 5 min with PBS-Tween 0,1%
Blocked for 30 min at room temprature with PBS-BSA 1%
Incubate with primary antibody in PBS-BSA 1% overnight at 4°C (antibody against sclerostin in this case)
Wash 3 times for 5 min with PBS-Tween 0,1%
Incubate with secondary antibody in PBS-BSA1% for 1h at room temperature in the dark (antibody anti-goat in this case)
Wash 3 times for 5 min with PBS-Tween 0,1%
Incubate with Phalloidin 488 PBS-BSA 1% 60 min at room temperature in the dark
Wash 3 times for 5 min with PBS-Tween 0,1%
Staining with DAPI for 15 min at room temprature in the dark
Wash 3 times for 5 min with PBS-Tween 0,1%
Air dry and mounting with fluorescent mounting medium

Abcam user community

Verified customer

投稿 Mar 11 2016

Good performance

Good Excellent 5/5 (Ease of Use)
Abreviews
Huh-7 cells: 30 min incubation including 5 min prior treatment with 0.1% Triton-X.

Dr. Teresa Otto

Verified customer

投稿 Jul 22 2015

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